Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33655 and RR42_RS33660 are separated by 43 nucleotides RR42_RS33660 and RR42_RS33665 are separated by 272 nucleotides
RR42_RS33655: RR42_RS33655 - ABC transporter substrate-binding protein, at 2,978,296 to 2,979,270
_RS33655
RR42_RS33660: RR42_RS33660 - dihydrofolate reductase, at 2,979,314 to 2,980,267
_RS33660
RR42_RS33665: RR42_RS33665 - gluconolactonase, at 2,980,540 to 2,981,442
_RS33665
Position (kb)
2979
2980
2981 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2978.532 kb on - strand, within RR42_RS33655 at 2978.718 kb on - strand, within RR42_RS33655 at 2978.726 kb on + strand, within RR42_RS33655 at 2978.727 kb on - strand, within RR42_RS33655 at 2978.727 kb on - strand, within RR42_RS33655 at 2978.889 kb on - strand, within RR42_RS33655 at 2978.936 kb on + strand, within RR42_RS33655 at 2978.936 kb on + strand, within RR42_RS33655 at 2978.937 kb on - strand, within RR42_RS33655 at 2978.937 kb on - strand, within RR42_RS33655 at 2978.937 kb on - strand, within RR42_RS33655 at 2978.937 kb on - strand, within RR42_RS33655 at 2978.937 kb on - strand, within RR42_RS33655 at 2979.002 kb on + strand, within RR42_RS33655 at 2979.002 kb on + strand, within RR42_RS33655 at 2979.002 kb on + strand, within RR42_RS33655 at 2979.003 kb on - strand, within RR42_RS33655 at 2979.003 kb on - strand, within RR42_RS33655 at 2979.003 kb on - strand, within RR42_RS33655 at 2979.003 kb on - strand, within RR42_RS33655 at 2979.185 kb on + strand at 2979.186 kb on - strand at 2979.242 kb on + strand at 2979.243 kb on - strand at 2979.296 kb on + strand at 2979.742 kb on - strand, within RR42_RS33660 at 2979.742 kb on - strand, within RR42_RS33660 at 2979.851 kb on - strand, within RR42_RS33660 at 2980.155 kb on + strand, within RR42_RS33660 at 2980.197 kb on + strand at 2980.197 kb on + strand at 2980.197 kb on + strand at 2980.198 kb on - strand at 2980.267 kb on + strand at 2980.268 kb on - strand at 2980.268 kb on - strand at 2980.280 kb on - strand at 2980.280 kb on - strand at 2980.375 kb on - strand at 2980.385 kb on + strand at 2980.385 kb on + strand at 2980.385 kb on + strand at 2980.385 kb on + strand at 2980.385 kb on + strand at 2980.385 kb on + strand at 2980.385 kb on + strand at 2980.385 kb on + strand at 2980.385 kb on + strand at 2980.385 kb on + strand at 2980.385 kb on + strand at 2980.386 kb on - strand at 2980.386 kb on - strand at 2980.386 kb on - strand at 2980.386 kb on - strand at 2980.386 kb on - strand at 2980.386 kb on - strand at 2980.386 kb on - strand at 2980.386 kb on - strand at 2980.386 kb on - strand at 2980.395 kb on + strand at 2980.395 kb on + strand at 2980.395 kb on + strand at 2980.396 kb on - strand at 2980.396 kb on - strand at 2980.396 kb on - strand at 2980.492 kb on - strand at 2980.494 kb on + strand at 2980.495 kb on - strand at 2980.630 kb on + strand at 2980.630 kb on + strand at 2980.630 kb on + strand at 2980.631 kb on - strand, within RR42_RS33665 at 2980.693 kb on - strand, within RR42_RS33665 at 2980.946 kb on - strand, within RR42_RS33665 at 2981.035 kb on + strand, within RR42_RS33665 at 2981.035 kb on + strand, within RR42_RS33665 at 2981.035 kb on + strand, within RR42_RS33665 at 2981.036 kb on - strand, within RR42_RS33665 at 2981.036 kb on - strand, within RR42_RS33665 at 2981.036 kb on - strand, within RR42_RS33665 at 2981.036 kb on - strand, within RR42_RS33665 at 2981.083 kb on + strand, within RR42_RS33665 at 2981.083 kb on + strand, within RR42_RS33665 at 2981.084 kb on - strand, within RR42_RS33665 at 2981.186 kb on - strand, within RR42_RS33665 at 2981.186 kb on - strand, within RR42_RS33665 at 2981.227 kb on - strand, within RR42_RS33665
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,978,532 - RR42_RS33655 0.24 +1.2 2,978,718 - RR42_RS33655 0.43 -0.8 2,978,726 + RR42_RS33655 0.44 +0.5 2,978,727 - RR42_RS33655 0.44 +0.4 2,978,727 - RR42_RS33655 0.44 -0.6 2,978,889 - RR42_RS33655 0.61 -0.2 2,978,936 + RR42_RS33655 0.66 +0.4 2,978,936 + RR42_RS33655 0.66 -0.2 2,978,937 - RR42_RS33655 0.66 -0.2 2,978,937 - RR42_RS33655 0.66 -1.3 2,978,937 - RR42_RS33655 0.66 +0.4 2,978,937 - RR42_RS33655 0.66 +0.2 2,978,937 - RR42_RS33655 0.66 -0.6 2,979,002 + RR42_RS33655 0.72 +0.4 2,979,002 + RR42_RS33655 0.72 +0.1 2,979,002 + RR42_RS33655 0.72 +0.9 2,979,003 - RR42_RS33655 0.73 +0.1 2,979,003 - RR42_RS33655 0.73 +0.1 2,979,003 - RR42_RS33655 0.73 -0.7 2,979,003 - RR42_RS33655 0.73 +1.5 2,979,185 + +0.3 2,979,186 - -1.2 2,979,242 + +0.3 2,979,243 - -0.4 2,979,296 + +1.0 2,979,742 - RR42_RS33660 0.45 -1.4 2,979,742 - RR42_RS33660 0.45 -0.2 2,979,851 - RR42_RS33660 0.56 +0.1 2,980,155 + RR42_RS33660 0.88 -0.7 2,980,197 + +0.5 2,980,197 + +0.4 2,980,197 + +1.8 2,980,198 - +0.5 2,980,267 + -0.9 2,980,268 - -1.8 2,980,268 - -1.1 2,980,280 - +0.7 2,980,280 - -0.4 2,980,375 - +0.3 2,980,385 + +0.2 2,980,385 + +0.1 2,980,385 + +1.8 2,980,385 + -0.6 2,980,385 + -0.6 2,980,385 + -1.4 2,980,385 + -1.8 2,980,385 + -0.1 2,980,385 + -0.3 2,980,385 + +0.3 2,980,385 + -0.9 2,980,386 - +0.2 2,980,386 - +0.3 2,980,386 - +0.2 2,980,386 - +0.9 2,980,386 - -0.2 2,980,386 - +0.4 2,980,386 - -0.1 2,980,386 - -1.0 2,980,386 - -1.7 2,980,395 + -0.3 2,980,395 + +0.3 2,980,395 + +1.0 2,980,396 - +0.4 2,980,396 - +0.3 2,980,396 - +0.1 2,980,492 - +0.4 2,980,494 + +0.5 2,980,495 - +1.0 2,980,630 + -0.0 2,980,630 + +0.6 2,980,630 + +0.4 2,980,631 - RR42_RS33665 0.10 +0.0 2,980,693 - RR42_RS33665 0.17 -0.9 2,980,946 - RR42_RS33665 0.45 +1.2 2,981,035 + RR42_RS33665 0.55 +0.1 2,981,035 + RR42_RS33665 0.55 +0.8 2,981,035 + RR42_RS33665 0.55 -0.8 2,981,036 - RR42_RS33665 0.55 +0.5 2,981,036 - RR42_RS33665 0.55 +0.4 2,981,036 - RR42_RS33665 0.55 +0.1 2,981,036 - RR42_RS33665 0.55 +0.1 2,981,083 + RR42_RS33665 0.60 -0.0 2,981,083 + RR42_RS33665 0.60 -0.5 2,981,084 - RR42_RS33665 0.60 +1.0 2,981,186 - RR42_RS33665 0.72 +0.2 2,981,186 - RR42_RS33665 0.72 +1.2 2,981,227 - RR42_RS33665 0.76 +0.8
Or see this region's nucleotide sequence