Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32355 and RR42_RS32360 are separated by 601 nucleotides RR42_RS32360 and RR42_RS32365 are separated by 878 nucleotides
RR42_RS32355: RR42_RS32355 - polysaccharide deacetylase, at 2,651,195 to 2,652,058
_RS32355
RR42_RS32360: RR42_RS32360 - hypothetical protein, at 2,652,660 to 2,653,226
_RS32360
RR42_RS32365: RR42_RS32365 - magnesium ABC transporter ATPase, at 2,654,105 to 2,656,861
_RS32365
Position (kb)
2652
2653
2654 Strain fitness (log2 ratio)
-2
-1
0
1 at 2651.695 kb on + strand, within RR42_RS32355 at 2651.695 kb on + strand, within RR42_RS32355 at 2652.377 kb on - strand at 2652.757 kb on + strand, within RR42_RS32360 at 2652.792 kb on - strand, within RR42_RS32360 at 2652.792 kb on - strand, within RR42_RS32360 at 2652.968 kb on - strand, within RR42_RS32360 at 2653.014 kb on - strand, within RR42_RS32360 at 2653.069 kb on + strand, within RR42_RS32360 at 2653.069 kb on + strand, within RR42_RS32360 at 2653.161 kb on - strand, within RR42_RS32360 at 2653.272 kb on + strand at 2653.273 kb on - strand at 2653.364 kb on + strand at 2653.365 kb on - strand at 2653.367 kb on + strand at 2653.368 kb on - strand at 2653.368 kb on - strand at 2653.368 kb on - strand at 2653.373 kb on + strand at 2653.374 kb on - strand at 2653.374 kb on - strand at 2653.374 kb on - strand at 2653.374 kb on - strand at 2653.374 kb on - strand at 2653.374 kb on - strand at 2653.430 kb on + strand at 2653.431 kb on - strand at 2653.431 kb on - strand at 2653.431 kb on - strand at 2653.439 kb on - strand at 2653.577 kb on + strand at 2653.587 kb on + strand at 2653.608 kb on + strand at 2653.658 kb on - strand at 2653.796 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,651,695 + RR42_RS32355 0.58 -0.7 2,651,695 + RR42_RS32355 0.58 +0.0 2,652,377 - +1.0 2,652,757 + RR42_RS32360 0.17 -0.3 2,652,792 - RR42_RS32360 0.23 -0.8 2,652,792 - RR42_RS32360 0.23 +0.1 2,652,968 - RR42_RS32360 0.54 -1.2 2,653,014 - RR42_RS32360 0.62 -0.7 2,653,069 + RR42_RS32360 0.72 -2.5 2,653,069 + RR42_RS32360 0.72 -0.3 2,653,161 - RR42_RS32360 0.88 -0.4 2,653,272 + -0.5 2,653,273 - -0.0 2,653,364 + +0.1 2,653,365 - +0.7 2,653,367 + +0.6 2,653,368 - +0.9 2,653,368 - +0.9 2,653,368 - +0.7 2,653,373 + -0.3 2,653,374 - -0.4 2,653,374 - +0.7 2,653,374 - -0.5 2,653,374 - +0.6 2,653,374 - +0.2 2,653,374 - +0.0 2,653,430 + -0.5 2,653,431 - +0.1 2,653,431 - -2.3 2,653,431 - +0.6 2,653,439 - +0.7 2,653,577 + -1.0 2,653,587 + -0.3 2,653,608 + +0.2 2,653,658 - +0.1 2,653,796 + -0.1
Or see this region's nucleotide sequence