Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31725 and RR42_RS31730 are separated by 41 nucleotides RR42_RS31730 and RR42_RS31735 overlap by 4 nucleotides RR42_RS31735 and RR42_RS31740 overlap by 4 nucleotides
RR42_RS31725: RR42_RS31725 - regulator, at 2,494,731 to 2,496,032
_RS31725
RR42_RS31730: RR42_RS31730 - formimidoylglutamase, at 2,496,074 to 2,497,015
_RS31730
RR42_RS31735: RR42_RS31735 - ABC transporter ATP-binding protein, at 2,497,012 to 2,497,773
_RS31735
RR42_RS31740: RR42_RS31740 - ABC transporter permease, at 2,497,770 to 2,498,438
_RS31740
Position (kb)
2496
2497
2498 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2495.355 kb on + strand, within RR42_RS31725 at 2495.355 kb on + strand, within RR42_RS31725 at 2495.355 kb on + strand, within RR42_RS31725 at 2495.356 kb on - strand, within RR42_RS31725 at 2495.356 kb on - strand, within RR42_RS31725 at 2495.356 kb on - strand, within RR42_RS31725 at 2495.356 kb on - strand, within RR42_RS31725 at 2495.559 kb on - strand, within RR42_RS31725 at 2495.559 kb on - strand, within RR42_RS31725 at 2495.565 kb on + strand, within RR42_RS31725 at 2495.583 kb on + strand, within RR42_RS31725 at 2495.719 kb on - strand, within RR42_RS31725 at 2495.754 kb on + strand, within RR42_RS31725 at 2495.755 kb on - strand, within RR42_RS31725 at 2495.851 kb on - strand, within RR42_RS31725 at 2496.030 kb on + strand at 2496.030 kb on + strand at 2496.135 kb on + strand at 2496.147 kb on + strand at 2496.148 kb on - strand at 2496.148 kb on - strand at 2496.148 kb on - strand at 2496.212 kb on + strand, within RR42_RS31730 at 2496.297 kb on + strand, within RR42_RS31730 at 2496.297 kb on + strand, within RR42_RS31730 at 2496.297 kb on + strand, within RR42_RS31730 at 2496.298 kb on - strand, within RR42_RS31730 at 2496.298 kb on - strand, within RR42_RS31730 at 2496.344 kb on - strand, within RR42_RS31730 at 2496.384 kb on + strand, within RR42_RS31730 at 2496.384 kb on + strand, within RR42_RS31730 at 2496.384 kb on + strand at 2496.385 kb on - strand, within RR42_RS31730 at 2496.427 kb on + strand, within RR42_RS31730 at 2496.444 kb on - strand, within RR42_RS31730 at 2496.459 kb on + strand, within RR42_RS31730 at 2496.459 kb on + strand, within RR42_RS31730 at 2496.459 kb on + strand, within RR42_RS31730 at 2496.459 kb on + strand, within RR42_RS31730 at 2496.460 kb on - strand, within RR42_RS31730 at 2496.460 kb on - strand, within RR42_RS31730 at 2496.765 kb on + strand, within RR42_RS31730 at 2496.766 kb on - strand, within RR42_RS31730 at 2497.101 kb on + strand, within RR42_RS31735 at 2497.255 kb on - strand, within RR42_RS31735 at 2497.255 kb on - strand, within RR42_RS31735 at 2497.791 kb on - strand at 2497.849 kb on + strand, within RR42_RS31740 at 2497.849 kb on + strand, within RR42_RS31740 at 2497.850 kb on - strand, within RR42_RS31740 at 2497.850 kb on - strand, within RR42_RS31740 at 2497.871 kb on - strand, within RR42_RS31740 at 2497.948 kb on - strand, within RR42_RS31740 at 2497.948 kb on - strand, within RR42_RS31740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,495,355 + RR42_RS31725 0.48 +0.4 2,495,355 + RR42_RS31725 0.48 -0.4 2,495,355 + RR42_RS31725 0.48 -0.3 2,495,356 - RR42_RS31725 0.48 +0.3 2,495,356 - RR42_RS31725 0.48 -1.0 2,495,356 - RR42_RS31725 0.48 +0.1 2,495,356 - RR42_RS31725 0.48 -0.3 2,495,559 - RR42_RS31725 0.64 -0.8 2,495,559 - RR42_RS31725 0.64 +0.2 2,495,565 + RR42_RS31725 0.64 +3.3 2,495,583 + RR42_RS31725 0.65 +0.3 2,495,719 - RR42_RS31725 0.76 +0.1 2,495,754 + RR42_RS31725 0.79 +0.5 2,495,755 - RR42_RS31725 0.79 +0.3 2,495,851 - RR42_RS31725 0.86 -0.1 2,496,030 + -0.1 2,496,030 + -0.1 2,496,135 + +0.6 2,496,147 + +0.3 2,496,148 - -0.3 2,496,148 - -1.0 2,496,148 - -0.2 2,496,212 + RR42_RS31730 0.15 +1.5 2,496,297 + RR42_RS31730 0.24 -0.3 2,496,297 + RR42_RS31730 0.24 +0.8 2,496,297 + RR42_RS31730 0.24 +0.3 2,496,298 - RR42_RS31730 0.24 -1.2 2,496,298 - RR42_RS31730 0.24 +0.6 2,496,344 - RR42_RS31730 0.29 -0.4 2,496,384 + RR42_RS31730 0.33 -0.1 2,496,384 + RR42_RS31730 0.33 -0.5 2,496,384 + -0.2 2,496,385 - RR42_RS31730 0.33 +0.2 2,496,427 + RR42_RS31730 0.37 -0.4 2,496,444 - RR42_RS31730 0.39 +0.4 2,496,459 + RR42_RS31730 0.41 -0.0 2,496,459 + RR42_RS31730 0.41 +0.6 2,496,459 + RR42_RS31730 0.41 -0.8 2,496,459 + RR42_RS31730 0.41 -0.3 2,496,460 - RR42_RS31730 0.41 -1.5 2,496,460 - RR42_RS31730 0.41 -0.3 2,496,765 + RR42_RS31730 0.73 +0.0 2,496,766 - RR42_RS31730 0.73 +0.9 2,497,101 + RR42_RS31735 0.12 +0.5 2,497,255 - RR42_RS31735 0.32 -0.8 2,497,255 - RR42_RS31735 0.32 -0.7 2,497,791 - +0.8 2,497,849 + RR42_RS31740 0.12 -1.1 2,497,849 + RR42_RS31740 0.12 -0.6 2,497,850 - RR42_RS31740 0.12 +0.8 2,497,850 - RR42_RS31740 0.12 +0.7 2,497,871 - RR42_RS31740 0.15 -1.2 2,497,948 - RR42_RS31740 0.27 +1.0 2,497,948 - RR42_RS31740 0.27 -0.2
Or see this region's nucleotide sequence