Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31035 and RR42_RS31040 are separated by 502 nucleotides RR42_RS31040 and RR42_RS31045 are separated by 23 nucleotides RR42_RS31045 and RR42_RS31050 are separated by 81 nucleotides
RR42_RS31035: RR42_RS31035 - CDP-alcohol phosphatidyltransferase, at 2,343,603 to 2,344,268
_RS31035
RR42_RS31040: RR42_RS31040 - hypothetical protein, at 2,344,771 to 2,345,037
_RS31040
RR42_RS31045: RR42_RS31045 - class III aminotransferase, at 2,345,061 to 2,345,903
_RS31045
RR42_RS31050: RR42_RS31050 - 3-hydroxyacyl-CoA dehydrogenase, at 2,345,985 to 2,346,956
_RS31050
Position (kb)
2344
2345
2346 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2343.786 kb on + strand, within RR42_RS31035 at 2343.786 kb on + strand, within RR42_RS31035 at 2344.003 kb on + strand, within RR42_RS31035 at 2344.004 kb on - strand, within RR42_RS31035 at 2344.258 kb on + strand at 2344.291 kb on + strand at 2344.291 kb on + strand at 2344.292 kb on - strand at 2344.292 kb on - strand at 2344.292 kb on - strand at 2344.292 kb on - strand at 2344.292 kb on - strand at 2344.292 kb on - strand at 2344.397 kb on + strand at 2344.397 kb on + strand at 2344.430 kb on + strand at 2344.430 kb on + strand at 2344.430 kb on + strand at 2344.460 kb on - strand at 2344.468 kb on - strand at 2344.709 kb on + strand at 2344.709 kb on + strand at 2344.709 kb on + strand at 2344.709 kb on + strand at 2344.709 kb on + strand at 2344.710 kb on - strand at 2344.710 kb on - strand at 2344.776 kb on + strand at 2344.776 kb on + strand at 2344.776 kb on + strand at 2344.777 kb on - strand at 2344.777 kb on - strand at 2344.871 kb on - strand, within RR42_RS31040 at 2344.910 kb on - strand, within RR42_RS31040 at 2344.910 kb on - strand, within RR42_RS31040 at 2344.910 kb on - strand, within RR42_RS31040 at 2344.910 kb on - strand, within RR42_RS31040 at 2345.068 kb on - strand at 2345.077 kb on + strand at 2345.077 kb on + strand at 2345.078 kb on - strand at 2345.305 kb on + strand, within RR42_RS31045 at 2345.305 kb on + strand, within RR42_RS31045 at 2345.305 kb on + strand, within RR42_RS31045 at 2345.306 kb on - strand, within RR42_RS31045 at 2345.306 kb on - strand, within RR42_RS31045 at 2345.343 kb on + strand, within RR42_RS31045 at 2345.344 kb on - strand, within RR42_RS31045 at 2345.344 kb on - strand, within RR42_RS31045 at 2345.611 kb on + strand, within RR42_RS31045 at 2345.612 kb on - strand, within RR42_RS31045 at 2345.692 kb on + strand, within RR42_RS31045 at 2345.692 kb on + strand, within RR42_RS31045 at 2345.839 kb on + strand at 2345.840 kb on - strand at 2345.862 kb on - strand at 2345.904 kb on - strand at 2345.904 kb on - strand at 2345.904 kb on - strand at 2345.904 kb on - strand at 2345.922 kb on + strand at 2345.923 kb on - strand at 2345.931 kb on + strand at 2345.932 kb on - strand at 2345.932 kb on - strand at 2345.940 kb on + strand at 2345.940 kb on + strand at 2345.941 kb on - strand at 2345.949 kb on + strand at 2345.959 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,343,786 + RR42_RS31035 0.27 +0.8 2,343,786 + RR42_RS31035 0.27 +1.4 2,344,003 + RR42_RS31035 0.60 -0.0 2,344,004 - RR42_RS31035 0.60 -0.4 2,344,258 + -0.7 2,344,291 + +0.1 2,344,291 + +0.7 2,344,292 - +0.5 2,344,292 - -0.6 2,344,292 - +0.1 2,344,292 - +0.3 2,344,292 - +0.4 2,344,292 - +0.1 2,344,397 + +0.6 2,344,397 + +1.1 2,344,430 + +0.1 2,344,430 + -0.8 2,344,430 + -0.1 2,344,460 - +0.0 2,344,468 - +0.2 2,344,709 + +0.2 2,344,709 + +0.2 2,344,709 + +0.2 2,344,709 + -0.7 2,344,709 + -0.9 2,344,710 - +0.5 2,344,710 - -0.7 2,344,776 + +0.4 2,344,776 + -0.9 2,344,776 + +0.9 2,344,777 - -0.2 2,344,777 - +2.5 2,344,871 - RR42_RS31040 0.37 +0.2 2,344,910 - RR42_RS31040 0.52 -0.8 2,344,910 - RR42_RS31040 0.52 -1.2 2,344,910 - RR42_RS31040 0.52 +0.0 2,344,910 - RR42_RS31040 0.52 -0.6 2,345,068 - +0.4 2,345,077 + -1.7 2,345,077 + -0.3 2,345,078 - +0.6 2,345,305 + RR42_RS31045 0.29 +1.0 2,345,305 + RR42_RS31045 0.29 -0.3 2,345,305 + RR42_RS31045 0.29 -0.9 2,345,306 - RR42_RS31045 0.29 -0.1 2,345,306 - RR42_RS31045 0.29 +0.2 2,345,343 + RR42_RS31045 0.33 +0.4 2,345,344 - RR42_RS31045 0.34 +2.2 2,345,344 - RR42_RS31045 0.34 -1.1 2,345,611 + RR42_RS31045 0.65 -0.5 2,345,612 - RR42_RS31045 0.65 +0.6 2,345,692 + RR42_RS31045 0.75 -0.1 2,345,692 + RR42_RS31045 0.75 +0.5 2,345,839 + -0.4 2,345,840 - +0.0 2,345,862 - +1.1 2,345,904 - -1.7 2,345,904 - +0.1 2,345,904 - +1.0 2,345,904 - -0.2 2,345,922 + +0.3 2,345,923 - +0.0 2,345,931 + -0.3 2,345,932 - +0.0 2,345,932 - +0.4 2,345,940 + +0.6 2,345,940 + -0.9 2,345,941 - +0.2 2,345,949 + -1.9 2,345,959 - +0.7
Or see this region's nucleotide sequence