Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31030 and RR42_RS31035 are separated by 6 nucleotides RR42_RS31035 and RR42_RS31040 are separated by 502 nucleotides RR42_RS31040 and RR42_RS31045 are separated by 23 nucleotides
RR42_RS31030: RR42_RS31030 - hypothetical protein, at 2,341,806 to 2,343,596
_RS31030
RR42_RS31035: RR42_RS31035 - CDP-alcohol phosphatidyltransferase, at 2,343,603 to 2,344,268
_RS31035
RR42_RS31040: RR42_RS31040 - hypothetical protein, at 2,344,771 to 2,345,037
_RS31040
RR42_RS31045: RR42_RS31045 - class III aminotransferase, at 2,345,061 to 2,345,903
_RS31045
Position (kb)
2343
2344
2345 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2342.620 kb on + strand, within RR42_RS31030 at 2342.620 kb on + strand, within RR42_RS31030 at 2342.621 kb on - strand, within RR42_RS31030 at 2342.621 kb on - strand, within RR42_RS31030 at 2342.621 kb on - strand, within RR42_RS31030 at 2342.747 kb on + strand, within RR42_RS31030 at 2342.799 kb on - strand, within RR42_RS31030 at 2342.806 kb on + strand, within RR42_RS31030 at 2342.858 kb on - strand, within RR42_RS31030 at 2342.858 kb on - strand, within RR42_RS31030 at 2342.858 kb on - strand, within RR42_RS31030 at 2342.984 kb on + strand, within RR42_RS31030 at 2343.036 kb on - strand, within RR42_RS31030 at 2343.036 kb on - strand, within RR42_RS31030 at 2343.073 kb on + strand, within RR42_RS31030 at 2343.073 kb on + strand, within RR42_RS31030 at 2343.074 kb on - strand, within RR42_RS31030 at 2343.074 kb on - strand, within RR42_RS31030 at 2343.142 kb on + strand, within RR42_RS31030 at 2343.142 kb on + strand, within RR42_RS31030 at 2343.142 kb on + strand, within RR42_RS31030 at 2343.142 kb on + strand, within RR42_RS31030 at 2343.142 kb on + strand, within RR42_RS31030 at 2343.143 kb on - strand, within RR42_RS31030 at 2343.143 kb on - strand, within RR42_RS31030 at 2343.143 kb on - strand, within RR42_RS31030 at 2343.143 kb on - strand, within RR42_RS31030 at 2343.143 kb on - strand, within RR42_RS31030 at 2343.200 kb on - strand, within RR42_RS31030 at 2343.264 kb on + strand, within RR42_RS31030 at 2343.312 kb on - strand, within RR42_RS31030 at 2343.341 kb on + strand, within RR42_RS31030 at 2343.343 kb on + strand, within RR42_RS31030 at 2343.344 kb on - strand, within RR42_RS31030 at 2343.527 kb on - strand at 2343.527 kb on - strand at 2343.590 kb on + strand at 2343.598 kb on + strand at 2343.598 kb on + strand at 2343.599 kb on - strand at 2343.599 kb on - strand at 2343.599 kb on - strand at 2343.599 kb on - strand at 2343.599 kb on - strand at 2343.786 kb on + strand, within RR42_RS31035 at 2343.786 kb on + strand, within RR42_RS31035 at 2344.003 kb on + strand, within RR42_RS31035 at 2344.004 kb on - strand, within RR42_RS31035 at 2344.258 kb on + strand at 2344.291 kb on + strand at 2344.291 kb on + strand at 2344.292 kb on - strand at 2344.292 kb on - strand at 2344.292 kb on - strand at 2344.292 kb on - strand at 2344.292 kb on - strand at 2344.292 kb on - strand at 2344.397 kb on + strand at 2344.397 kb on + strand at 2344.430 kb on + strand at 2344.430 kb on + strand at 2344.430 kb on + strand at 2344.460 kb on - strand at 2344.468 kb on - strand at 2344.709 kb on + strand at 2344.709 kb on + strand at 2344.709 kb on + strand at 2344.709 kb on + strand at 2344.709 kb on + strand at 2344.710 kb on - strand at 2344.710 kb on - strand at 2344.776 kb on + strand at 2344.776 kb on + strand at 2344.776 kb on + strand at 2344.777 kb on - strand at 2344.777 kb on - strand at 2344.871 kb on - strand, within RR42_RS31040 at 2344.910 kb on - strand, within RR42_RS31040 at 2344.910 kb on - strand, within RR42_RS31040 at 2344.910 kb on - strand, within RR42_RS31040 at 2344.910 kb on - strand, within RR42_RS31040 at 2345.068 kb on - strand at 2345.077 kb on + strand at 2345.077 kb on + strand at 2345.078 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,342,620 + RR42_RS31030 0.45 -0.2 2,342,620 + RR42_RS31030 0.45 -0.1 2,342,621 - RR42_RS31030 0.46 +0.2 2,342,621 - RR42_RS31030 0.46 -0.4 2,342,621 - RR42_RS31030 0.46 -0.5 2,342,747 + RR42_RS31030 0.53 +0.4 2,342,799 - RR42_RS31030 0.55 +0.3 2,342,806 + RR42_RS31030 0.56 +0.1 2,342,858 - RR42_RS31030 0.59 -0.7 2,342,858 - RR42_RS31030 0.59 -0.4 2,342,858 - RR42_RS31030 0.59 -0.7 2,342,984 + RR42_RS31030 0.66 -1.1 2,343,036 - RR42_RS31030 0.69 -2.1 2,343,036 - RR42_RS31030 0.69 +0.2 2,343,073 + RR42_RS31030 0.71 +0.1 2,343,073 + RR42_RS31030 0.71 -2.1 2,343,074 - RR42_RS31030 0.71 -0.7 2,343,074 - RR42_RS31030 0.71 -1.0 2,343,142 + RR42_RS31030 0.75 -0.8 2,343,142 + RR42_RS31030 0.75 -1.0 2,343,142 + RR42_RS31030 0.75 +0.1 2,343,142 + RR42_RS31030 0.75 +0.1 2,343,142 + RR42_RS31030 0.75 -0.4 2,343,143 - RR42_RS31030 0.75 -1.8 2,343,143 - RR42_RS31030 0.75 -0.1 2,343,143 - RR42_RS31030 0.75 -0.1 2,343,143 - RR42_RS31030 0.75 +1.3 2,343,143 - RR42_RS31030 0.75 +0.1 2,343,200 - RR42_RS31030 0.78 -0.5 2,343,264 + RR42_RS31030 0.81 +0.9 2,343,312 - RR42_RS31030 0.84 -0.4 2,343,341 + RR42_RS31030 0.86 -0.4 2,343,343 + RR42_RS31030 0.86 +0.2 2,343,344 - RR42_RS31030 0.86 +0.0 2,343,527 - +1.2 2,343,527 - -0.7 2,343,590 + -0.6 2,343,598 + +0.5 2,343,598 + -0.8 2,343,599 - -0.3 2,343,599 - +0.7 2,343,599 - -0.3 2,343,599 - +0.8 2,343,599 - +0.2 2,343,786 + RR42_RS31035 0.27 +0.8 2,343,786 + RR42_RS31035 0.27 +1.4 2,344,003 + RR42_RS31035 0.60 -0.0 2,344,004 - RR42_RS31035 0.60 -0.4 2,344,258 + -0.7 2,344,291 + +0.1 2,344,291 + +0.7 2,344,292 - +0.5 2,344,292 - -0.6 2,344,292 - +0.1 2,344,292 - +0.3 2,344,292 - +0.4 2,344,292 - +0.1 2,344,397 + +0.6 2,344,397 + +1.1 2,344,430 + +0.1 2,344,430 + -0.8 2,344,430 + -0.1 2,344,460 - +0.0 2,344,468 - +0.2 2,344,709 + +0.2 2,344,709 + +0.2 2,344,709 + +0.2 2,344,709 + -0.7 2,344,709 + -0.9 2,344,710 - +0.5 2,344,710 - -0.7 2,344,776 + +0.4 2,344,776 + -0.9 2,344,776 + +0.9 2,344,777 - -0.2 2,344,777 - +2.5 2,344,871 - RR42_RS31040 0.37 +0.2 2,344,910 - RR42_RS31040 0.52 -0.8 2,344,910 - RR42_RS31040 0.52 -1.2 2,344,910 - RR42_RS31040 0.52 +0.0 2,344,910 - RR42_RS31040 0.52 -0.6 2,345,068 - +0.4 2,345,077 + -1.7 2,345,077 + -0.3 2,345,078 - +0.6
Or see this region's nucleotide sequence