Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30970 and RR42_RS30975 are separated by 29 nucleotides RR42_RS30975 and RR42_RS30980 are separated by 65 nucleotides RR42_RS30980 and RR42_RS30985 are separated by 19 nucleotides RR42_RS30985 and RR42_RS30990 are separated by 250 nucleotides
RR42_RS30970: RR42_RS30970 - hypothetical protein, at 2,332,213 to 2,332,866
_RS30970
RR42_RS30975: RR42_RS30975 - blue copper protein, at 2,332,896 to 2,333,405
_RS30975
RR42_RS30980: RR42_RS30980 - hypothetical protein, at 2,333,471 to 2,333,830
_RS30980
RR42_RS30985: RR42_RS30985 - histone-like nucleoid-structuring protein, at 2,333,850 to 2,334,182
_RS30985
RR42_RS30990: RR42_RS30990 - membrane protein, at 2,334,433 to 2,334,831
_RS30990
Position (kb)
2333
2334 Strain fitness (log2 ratio)
-1
0
1
2 at 2332.621 kb on + strand, within RR42_RS30970 at 2332.621 kb on + strand, within RR42_RS30970 at 2332.622 kb on - strand, within RR42_RS30970 at 2332.622 kb on - strand, within RR42_RS30970 at 2332.622 kb on - strand, within RR42_RS30970 at 2332.667 kb on - strand, within RR42_RS30970 at 2332.838 kb on - strand at 2332.838 kb on - strand at 2332.838 kb on - strand at 2333.123 kb on + strand, within RR42_RS30975 at 2333.227 kb on - strand, within RR42_RS30975 at 2333.227 kb on - strand, within RR42_RS30975 at 2333.321 kb on + strand, within RR42_RS30975 at 2333.368 kb on - strand at 2333.368 kb on - strand at 2333.438 kb on + strand at 2333.438 kb on + strand at 2333.457 kb on - strand at 2333.628 kb on + strand, within RR42_RS30980 at 2333.629 kb on - strand, within RR42_RS30980 at 2333.841 kb on - strand at 2334.156 kb on + strand at 2334.156 kb on + strand at 2334.192 kb on - strand at 2334.243 kb on - strand at 2334.279 kb on + strand at 2334.434 kb on + strand at 2334.476 kb on - strand, within RR42_RS30990 at 2334.560 kb on + strand, within RR42_RS30990 at 2334.560 kb on + strand, within RR42_RS30990 at 2334.560 kb on + strand, within RR42_RS30990 at 2334.709 kb on + strand, within RR42_RS30990 at 2334.709 kb on + strand, within RR42_RS30990 at 2334.710 kb on - strand, within RR42_RS30990 at 2334.746 kb on + strand, within RR42_RS30990 at 2334.746 kb on + strand, within RR42_RS30990 at 2334.746 kb on + strand, within RR42_RS30990 at 2334.747 kb on - strand, within RR42_RS30990 at 2334.748 kb on + strand, within RR42_RS30990 at 2334.749 kb on - strand, within RR42_RS30990 at 2334.791 kb on + strand, within RR42_RS30990 at 2334.791 kb on + strand, within RR42_RS30990 at 2334.792 kb on - strand at 2334.792 kb on - strand at 2334.800 kb on + strand at 2334.801 kb on - strand at 2334.806 kb on + strand at 2334.807 kb on - strand at 2334.807 kb on - strand at 2334.807 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,332,621 + RR42_RS30970 0.62 +0.7 2,332,621 + RR42_RS30970 0.62 -0.6 2,332,622 - RR42_RS30970 0.63 +0.8 2,332,622 - RR42_RS30970 0.63 +0.4 2,332,622 - RR42_RS30970 0.63 -0.9 2,332,667 - RR42_RS30970 0.69 -0.4 2,332,838 - +1.2 2,332,838 - -0.2 2,332,838 - -0.4 2,333,123 + RR42_RS30975 0.45 +1.7 2,333,227 - RR42_RS30975 0.65 +0.2 2,333,227 - RR42_RS30975 0.65 -0.1 2,333,321 + RR42_RS30975 0.83 +0.1 2,333,368 - +0.3 2,333,368 - -0.7 2,333,438 + -0.3 2,333,438 + +0.5 2,333,457 - +1.0 2,333,628 + RR42_RS30980 0.44 -1.6 2,333,629 - RR42_RS30980 0.44 -0.4 2,333,841 - +0.6 2,334,156 + -0.1 2,334,156 + +0.2 2,334,192 - -0.9 2,334,243 - +0.7 2,334,279 + -1.1 2,334,434 + -0.2 2,334,476 - RR42_RS30990 0.11 -0.2 2,334,560 + RR42_RS30990 0.32 -0.3 2,334,560 + RR42_RS30990 0.32 -0.4 2,334,560 + RR42_RS30990 0.32 -0.5 2,334,709 + RR42_RS30990 0.69 +0.3 2,334,709 + RR42_RS30990 0.69 +0.5 2,334,710 - RR42_RS30990 0.69 -0.2 2,334,746 + RR42_RS30990 0.78 +0.5 2,334,746 + RR42_RS30990 0.78 +0.4 2,334,746 + RR42_RS30990 0.78 +0.3 2,334,747 - RR42_RS30990 0.79 +0.3 2,334,748 + RR42_RS30990 0.79 +0.6 2,334,749 - RR42_RS30990 0.79 -0.7 2,334,791 + RR42_RS30990 0.90 -1.0 2,334,791 + RR42_RS30990 0.90 -1.0 2,334,792 - +1.0 2,334,792 - +0.8 2,334,800 + -0.9 2,334,801 - +2.4 2,334,806 + -0.2 2,334,807 - -0.6 2,334,807 - -1.2 2,334,807 - -0.1
Or see this region's nucleotide sequence