Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30730 and RR42_RS30735 are separated by 501 nucleotides RR42_RS30735 and RR42_RS30740 are separated by 141 nucleotides RR42_RS30740 and RR42_RS30745 are separated by 252 nucleotides
RR42_RS30730: RR42_RS30730 - hypothetical protein, at 2,271,144 to 2,272,472
_RS30730
RR42_RS30735: RR42_RS30735 - tRNA-Met, at 2,272,974 to 2,273,049
_RS30735
RR42_RS30740: RR42_RS30740 - hypothetical protein, at 2,273,191 to 2,273,403
_RS30740
RR42_RS30745: RR42_RS30745 - hypothetical protein, at 2,273,656 to 2,275,734
_RS30745
Position (kb)
2272
2273
2274 Strain fitness (log2 ratio)
-2
-1
0
1 at 2272.138 kb on + strand, within RR42_RS30730 at 2272.138 kb on + strand, within RR42_RS30730 at 2272.139 kb on - strand, within RR42_RS30730 at 2272.139 kb on - strand, within RR42_RS30730 at 2272.182 kb on + strand, within RR42_RS30730 at 2272.300 kb on - strand, within RR42_RS30730 at 2272.324 kb on + strand, within RR42_RS30730 at 2272.482 kb on + strand at 2272.482 kb on + strand at 2272.483 kb on - strand at 2272.483 kb on - strand at 2272.483 kb on - strand at 2272.652 kb on + strand at 2272.709 kb on + strand at 2272.741 kb on + strand at 2272.741 kb on + strand at 2272.778 kb on + strand at 2272.778 kb on + strand at 2272.778 kb on + strand at 2272.779 kb on - strand at 2272.779 kb on - strand at 2272.779 kb on - strand at 2273.013 kb on + strand, within RR42_RS30735 at 2273.058 kb on + strand at 2273.060 kb on + strand at 2273.060 kb on + strand at 2273.060 kb on + strand at 2273.061 kb on - strand at 2273.061 kb on - strand at 2273.089 kb on + strand at 2273.090 kb on - strand at 2273.155 kb on + strand at 2273.155 kb on + strand at 2273.156 kb on - strand at 2273.156 kb on - strand at 2273.156 kb on - strand at 2273.293 kb on + strand, within RR42_RS30740 at 2273.294 kb on - strand, within RR42_RS30740 at 2273.368 kb on + strand, within RR42_RS30740 at 2273.368 kb on + strand, within RR42_RS30740 at 2273.368 kb on + strand, within RR42_RS30740 at 2273.369 kb on - strand, within RR42_RS30740 at 2273.369 kb on - strand, within RR42_RS30740 at 2273.369 kb on - strand, within RR42_RS30740 at 2273.369 kb on - strand, within RR42_RS30740 at 2273.369 kb on - strand, within RR42_RS30740 at 2273.372 kb on + strand, within RR42_RS30740 at 2273.372 kb on + strand, within RR42_RS30740 at 2273.373 kb on - strand, within RR42_RS30740 at 2273.373 kb on - strand, within RR42_RS30740 at 2273.373 kb on - strand, within RR42_RS30740 at 2273.373 kb on - strand, within RR42_RS30740 at 2273.381 kb on + strand, within RR42_RS30740 at 2273.382 kb on - strand at 2273.453 kb on + strand at 2273.454 kb on - strand at 2273.454 kb on - strand at 2273.454 kb on - strand at 2273.569 kb on - strand at 2273.569 kb on - strand at 2273.582 kb on + strand at 2273.582 kb on + strand at 2273.582 kb on + strand at 2273.625 kb on + strand at 2273.625 kb on + strand at 2273.626 kb on - strand at 2273.626 kb on - strand at 2273.626 kb on - strand at 2273.630 kb on - strand at 2273.635 kb on + strand at 2273.635 kb on + strand at 2273.635 kb on + strand at 2273.638 kb on + strand at 2273.639 kb on - strand at 2273.661 kb on + strand at 2273.662 kb on - strand at 2273.664 kb on + strand at 2273.665 kb on - strand at 2273.665 kb on - strand at 2273.738 kb on + strand at 2273.739 kb on - strand at 2273.798 kb on + strand at 2273.832 kb on + strand at 2273.857 kb on + strand at 2273.857 kb on + strand at 2273.858 kb on - strand at 2273.989 kb on + strand, within RR42_RS30745 at 2273.990 kb on - strand, within RR42_RS30745 at 2273.990 kb on - strand, within RR42_RS30745 at 2273.990 kb on - strand, within RR42_RS30745 at 2273.990 kb on - strand, within RR42_RS30745 at 2273.990 kb on - strand, within RR42_RS30745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,272,138 + RR42_RS30730 0.75 +0.0 2,272,138 + RR42_RS30730 0.75 +0.4 2,272,139 - RR42_RS30730 0.75 -0.4 2,272,139 - RR42_RS30730 0.75 -0.5 2,272,182 + RR42_RS30730 0.78 +0.8 2,272,300 - RR42_RS30730 0.87 -0.0 2,272,324 + RR42_RS30730 0.89 -0.5 2,272,482 + +0.9 2,272,482 + +0.0 2,272,483 - -0.7 2,272,483 - -0.2 2,272,483 - +0.2 2,272,652 + -1.4 2,272,709 + +0.6 2,272,741 + -0.5 2,272,741 + +0.6 2,272,778 + +0.4 2,272,778 + +0.2 2,272,778 + +0.6 2,272,779 - +0.1 2,272,779 - -1.2 2,272,779 - -0.1 2,273,013 + RR42_RS30735 0.51 -0.7 2,273,058 + -0.2 2,273,060 + +0.2 2,273,060 + -0.2 2,273,060 + -1.4 2,273,061 - +0.3 2,273,061 - +0.1 2,273,089 + -0.5 2,273,090 - -0.5 2,273,155 + +0.4 2,273,155 + +0.8 2,273,156 - +0.2 2,273,156 - -0.3 2,273,156 - +1.7 2,273,293 + RR42_RS30740 0.48 -0.1 2,273,294 - RR42_RS30740 0.48 +0.9 2,273,368 + RR42_RS30740 0.83 -0.2 2,273,368 + RR42_RS30740 0.83 -1.3 2,273,368 + RR42_RS30740 0.83 -2.0 2,273,369 - RR42_RS30740 0.84 +0.5 2,273,369 - RR42_RS30740 0.84 +0.2 2,273,369 - RR42_RS30740 0.84 +1.6 2,273,369 - RR42_RS30740 0.84 +0.5 2,273,369 - RR42_RS30740 0.84 +0.0 2,273,372 + RR42_RS30740 0.85 -0.7 2,273,372 + RR42_RS30740 0.85 +0.4 2,273,373 - RR42_RS30740 0.85 +0.6 2,273,373 - RR42_RS30740 0.85 +0.9 2,273,373 - RR42_RS30740 0.85 +0.4 2,273,373 - RR42_RS30740 0.85 -0.5 2,273,381 + RR42_RS30740 0.89 +0.3 2,273,382 - -0.9 2,273,453 + +0.1 2,273,454 - +0.9 2,273,454 - -0.4 2,273,454 - +0.6 2,273,569 - +0.2 2,273,569 - +0.7 2,273,582 + -1.4 2,273,582 + -0.8 2,273,582 + +0.5 2,273,625 + +0.4 2,273,625 + -0.2 2,273,626 - -0.0 2,273,626 - +0.0 2,273,626 - -0.3 2,273,630 - +0.0 2,273,635 + +0.7 2,273,635 + +0.4 2,273,635 + +0.0 2,273,638 + +0.6 2,273,639 - +0.2 2,273,661 + +0.4 2,273,662 - -0.5 2,273,664 + -1.1 2,273,665 - -2.2 2,273,665 - -0.0 2,273,738 + +0.7 2,273,739 - +0.3 2,273,798 + -0.2 2,273,832 + +0.5 2,273,857 + +0.3 2,273,857 + +0.1 2,273,858 - -0.4 2,273,989 + RR42_RS30745 0.16 +0.5 2,273,990 - RR42_RS30745 0.16 +0.0 2,273,990 - RR42_RS30745 0.16 +0.5 2,273,990 - RR42_RS30745 0.16 -2.5 2,273,990 - RR42_RS30745 0.16 +0.4 2,273,990 - RR42_RS30745 0.16 +0.7
Or see this region's nucleotide sequence