Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28185 and RR42_RS28190 are separated by 84 nucleotides RR42_RS28190 and RR42_RS28195 are separated by 73 nucleotides RR42_RS28195 and RR42_RS28200 overlap by 8 nucleotides RR42_RS28200 and RR42_RS28205 are separated by 168 nucleotides
RR42_RS28185: RR42_RS28185 - GntR family transcriptional regulator, at 1,679,978 to 1,681,423
_RS28185
RR42_RS28190: RR42_RS28190 - MerR family transcriptional regulator, at 1,681,508 to 1,681,945
_RS28190
RR42_RS28195: RR42_RS28195 - SAM-dependent methyltransferase, at 1,682,019 to 1,682,897
_RS28195
RR42_RS28200: RR42_RS28200 - membrane protein, at 1,682,890 to 1,683,411
_RS28200
RR42_RS28205: RR42_RS28205 - hypothetical protein, at 1,683,580 to 1,685,718
_RS28205
Position (kb)
1682
1683 Strain fitness (log2 ratio)
-1
0
1 at 1681.139 kb on + strand, within RR42_RS28185 at 1681.140 kb on - strand, within RR42_RS28185 at 1681.140 kb on - strand, within RR42_RS28185 at 1681.320 kb on - strand at 1681.320 kb on - strand at 1681.320 kb on - strand at 1681.322 kb on + strand at 1681.322 kb on + strand at 1681.323 kb on - strand at 1681.464 kb on + strand at 1681.852 kb on + strand, within RR42_RS28190 at 1681.878 kb on + strand, within RR42_RS28190 at 1681.928 kb on + strand at 1681.928 kb on + strand at 1681.928 kb on + strand at 1681.929 kb on - strand at 1681.935 kb on - strand at 1681.982 kb on + strand at 1681.982 kb on + strand at 1681.982 kb on + strand at 1681.982 kb on + strand at 1681.982 kb on + strand at 1681.983 kb on - strand at 1681.983 kb on - strand at 1682.701 kb on - strand, within RR42_RS28195 at 1682.798 kb on + strand, within RR42_RS28195 at 1682.799 kb on + strand, within RR42_RS28195 at 1682.799 kb on + strand, within RR42_RS28195 at 1682.908 kb on + strand at 1682.909 kb on - strand at 1683.121 kb on + strand, within RR42_RS28200 at 1683.156 kb on - strand, within RR42_RS28200 at 1683.285 kb on + strand, within RR42_RS28200 at 1683.285 kb on + strand, within RR42_RS28200 at 1683.286 kb on - strand, within RR42_RS28200 at 1683.315 kb on + strand, within RR42_RS28200 at 1683.316 kb on - strand, within RR42_RS28200 at 1683.352 kb on + strand, within RR42_RS28200 at 1683.503 kb on + strand at 1683.504 kb on - strand at 1683.508 kb on + strand at 1683.510 kb on + strand at 1683.511 kb on - strand at 1683.549 kb on + strand at 1683.580 kb on - strand at 1683.688 kb on - strand at 1683.751 kb on + strand at 1683.751 kb on + strand at 1683.769 kb on + strand at 1683.822 kb on + strand, within RR42_RS28205 at 1683.880 kb on + strand, within RR42_RS28205
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 1,681,139 + RR42_RS28185 0.80 -0.7 1,681,140 - RR42_RS28185 0.80 +0.2 1,681,140 - RR42_RS28185 0.80 -1.2 1,681,320 - +0.2 1,681,320 - -0.1 1,681,320 - +0.9 1,681,322 + +0.2 1,681,322 + +0.2 1,681,323 - +1.1 1,681,464 + -0.1 1,681,852 + RR42_RS28190 0.79 +0.3 1,681,878 + RR42_RS28190 0.84 -1.5 1,681,928 + -1.0 1,681,928 + +0.4 1,681,928 + -0.5 1,681,929 - +0.2 1,681,935 - +0.3 1,681,982 + +0.7 1,681,982 + +0.6 1,681,982 + +0.6 1,681,982 + +0.9 1,681,982 + -0.4 1,681,983 - -1.0 1,681,983 - -0.4 1,682,701 - RR42_RS28195 0.78 -0.8 1,682,798 + RR42_RS28195 0.89 -0.7 1,682,799 + RR42_RS28195 0.89 -0.5 1,682,799 + RR42_RS28195 0.89 -0.2 1,682,908 + +0.3 1,682,909 - +0.8 1,683,121 + RR42_RS28200 0.44 +0.9 1,683,156 - RR42_RS28200 0.51 +0.6 1,683,285 + RR42_RS28200 0.76 +0.2 1,683,285 + RR42_RS28200 0.76 -0.4 1,683,286 - RR42_RS28200 0.76 +0.6 1,683,315 + RR42_RS28200 0.81 -0.5 1,683,316 - RR42_RS28200 0.82 -0.3 1,683,352 + RR42_RS28200 0.89 -0.2 1,683,503 + -0.4 1,683,504 - -0.8 1,683,508 + -0.3 1,683,510 + +0.0 1,683,511 - -0.6 1,683,549 + +0.2 1,683,580 - -0.5 1,683,688 - -0.1 1,683,751 + -1.0 1,683,751 + -1.1 1,683,769 + +1.6 1,683,822 + RR42_RS28205 0.11 -1.0 1,683,880 + RR42_RS28205 0.14 +0.6
Or see this region's nucleotide sequence