Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27165 and RR42_RS27170 are separated by 86 nucleotides RR42_RS27170 and RR42_RS27175 overlap by 28 nucleotides RR42_RS27175 and RR42_RS27180 are separated by 168 nucleotides RR42_RS27180 and RR42_RS27185 are separated by 201 nucleotides
RR42_RS27165: RR42_RS27165 - hypothetical protein, at 1,446,618 to 1,447,166
_RS27165
RR42_RS27170: RR42_RS27170 - ketopantoate reductase, at 1,447,253 to 1,448,191
_RS27170
RR42_RS27175: RR42_RS27175 - AraC family transcriptional regulator, at 1,448,164 to 1,449,162
_RS27175
RR42_RS27180: RR42_RS27180 - alkylhydroperoxidase, at 1,449,331 to 1,449,867
_RS27180
RR42_RS27185: RR42_RS27185 - sodium:proton antiporter, at 1,450,069 to 1,451,316
_RS27185
Position (kb)
1448
1449
1450 Strain fitness (log2 ratio)
-2
-1
0
1 at 1447.201 kb on + strand at 1447.201 kb on + strand at 1447.202 kb on - strand at 1447.202 kb on - strand at 1447.229 kb on + strand at 1447.292 kb on + strand at 1447.292 kb on + strand at 1447.454 kb on + strand, within RR42_RS27170 at 1447.455 kb on - strand, within RR42_RS27170 at 1447.485 kb on - strand, within RR42_RS27170 at 1447.583 kb on + strand, within RR42_RS27170 at 1447.605 kb on - strand, within RR42_RS27170 at 1447.637 kb on + strand, within RR42_RS27170 at 1447.637 kb on + strand, within RR42_RS27170 at 1447.638 kb on - strand, within RR42_RS27170 at 1447.638 kb on - strand, within RR42_RS27170 at 1448.076 kb on - strand, within RR42_RS27170 at 1448.076 kb on - strand, within RR42_RS27170 at 1448.147 kb on + strand at 1448.147 kb on + strand at 1448.149 kb on + strand at 1448.150 kb on - strand at 1448.150 kb on - strand at 1448.312 kb on + strand, within RR42_RS27175 at 1448.313 kb on - strand, within RR42_RS27175 at 1448.568 kb on + strand, within RR42_RS27175 at 1448.569 kb on - strand, within RR42_RS27175 at 1448.569 kb on - strand, within RR42_RS27175 at 1448.940 kb on + strand, within RR42_RS27175 at 1449.230 kb on - strand at 1449.259 kb on - strand at 1449.348 kb on + strand at 1449.348 kb on + strand at 1449.349 kb on - strand at 1449.349 kb on - strand at 1449.349 kb on - strand at 1449.431 kb on - strand, within RR42_RS27180 at 1449.568 kb on + strand, within RR42_RS27180 at 1449.568 kb on + strand, within RR42_RS27180 at 1449.569 kb on - strand, within RR42_RS27180 at 1449.569 kb on - strand, within RR42_RS27180 at 1449.816 kb on + strand at 1449.817 kb on - strand at 1449.994 kb on + strand at 1449.994 kb on + strand at 1450.025 kb on + strand at 1450.025 kb on + strand at 1450.026 kb on - strand at 1450.063 kb on + strand at 1450.063 kb on + strand at 1450.063 kb on + strand at 1450.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 1,447,201 + +0.1 1,447,201 + -0.1 1,447,202 - +1.0 1,447,202 - +0.1 1,447,229 + +0.4 1,447,292 + +0.3 1,447,292 + -1.2 1,447,454 + RR42_RS27170 0.21 -0.6 1,447,455 - RR42_RS27170 0.22 -0.3 1,447,485 - RR42_RS27170 0.25 -1.5 1,447,583 + RR42_RS27170 0.35 -0.4 1,447,605 - RR42_RS27170 0.37 -1.9 1,447,637 + RR42_RS27170 0.41 -0.3 1,447,637 + RR42_RS27170 0.41 +0.1 1,447,638 - RR42_RS27170 0.41 -1.1 1,447,638 - RR42_RS27170 0.41 +0.0 1,448,076 - RR42_RS27170 0.88 -0.8 1,448,076 - RR42_RS27170 0.88 -0.2 1,448,147 + -0.6 1,448,147 + -0.0 1,448,149 + +0.4 1,448,150 - -0.1 1,448,150 - -1.2 1,448,312 + RR42_RS27175 0.15 -0.0 1,448,313 - RR42_RS27175 0.15 -0.8 1,448,568 + RR42_RS27175 0.40 -0.2 1,448,569 - RR42_RS27175 0.41 +0.3 1,448,569 - RR42_RS27175 0.41 -1.5 1,448,940 + RR42_RS27175 0.78 -1.2 1,449,230 - -0.5 1,449,259 - +1.7 1,449,348 + +0.5 1,449,348 + -0.1 1,449,349 - +0.6 1,449,349 - +0.3 1,449,349 - -1.3 1,449,431 - RR42_RS27180 0.19 +1.2 1,449,568 + RR42_RS27180 0.44 -0.0 1,449,568 + RR42_RS27180 0.44 -0.4 1,449,569 - RR42_RS27180 0.44 -0.9 1,449,569 - RR42_RS27180 0.44 -0.4 1,449,816 + +0.7 1,449,817 - +0.4 1,449,994 + +0.3 1,449,994 + +0.2 1,450,025 + -0.6 1,450,025 + -0.8 1,450,026 - -1.5 1,450,063 + +0.1 1,450,063 + +0.6 1,450,063 + +0.6 1,450,158 - -0.9
Or see this region's nucleotide sequence