Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25615 and RR42_RS25620 are separated by 6 nucleotides RR42_RS25620 and RR42_RS25625 are separated by 80 nucleotides RR42_RS25625 and RR42_RS25630 are separated by 161 nucleotides
RR42_RS25615: RR42_RS25615 - TetR family transcriptional regulator, at 1,090,802 to 1,091,383
_RS25615
RR42_RS25620: RR42_RS25620 - hypothetical protein, at 1,091,390 to 1,091,926
_RS25620
RR42_RS25625: RR42_RS25625 - LysR family transcriptional regulator, at 1,092,007 to 1,092,963
_RS25625
RR42_RS25630: RR42_RS25630 - transporter, at 1,093,125 to 1,094,438
_RS25630
Position (kb)
1092
1093 Strain fitness (log2 ratio)
-1
0
1 at 1091.055 kb on - strand, within RR42_RS25615 at 1091.100 kb on + strand, within RR42_RS25615 at 1091.212 kb on + strand, within RR42_RS25615 at 1091.381 kb on + strand at 1091.382 kb on - strand at 1091.382 kb on - strand at 1091.685 kb on + strand, within RR42_RS25620 at 1091.685 kb on + strand, within RR42_RS25620 at 1091.685 kb on + strand, within RR42_RS25620 at 1091.685 kb on + strand, within RR42_RS25620 at 1091.701 kb on + strand, within RR42_RS25620 at 1091.701 kb on + strand, within RR42_RS25620 at 1091.704 kb on - strand, within RR42_RS25620 at 1091.887 kb on - strand at 1091.887 kb on - strand at 1091.921 kb on + strand at 1092.083 kb on - strand at 1092.083 kb on - strand at 1092.097 kb on + strand at 1092.098 kb on - strand at 1092.098 kb on - strand at 1092.158 kb on - strand, within RR42_RS25625 at 1092.164 kb on - strand, within RR42_RS25625 at 1092.164 kb on - strand, within RR42_RS25625 at 1093.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 1,091,055 - RR42_RS25615 0.43 -1.2 1,091,100 + RR42_RS25615 0.51 -0.2 1,091,212 + RR42_RS25615 0.70 -1.0 1,091,381 + -0.2 1,091,382 - -1.2 1,091,382 - +0.2 1,091,685 + RR42_RS25620 0.55 +0.5 1,091,685 + RR42_RS25620 0.55 -0.2 1,091,685 + RR42_RS25620 0.55 +0.1 1,091,685 + RR42_RS25620 0.55 +0.0 1,091,701 + RR42_RS25620 0.58 +0.7 1,091,701 + RR42_RS25620 0.58 +1.6 1,091,704 - RR42_RS25620 0.58 -0.8 1,091,887 - +0.5 1,091,887 - -0.7 1,091,921 + +1.7 1,092,083 - +0.1 1,092,083 - +0.2 1,092,097 + +0.0 1,092,098 - -1.0 1,092,098 - -0.0 1,092,158 - RR42_RS25625 0.16 -1.3 1,092,164 - RR42_RS25625 0.16 -1.5 1,092,164 - RR42_RS25625 0.16 +0.1 1,093,049 - +0.6
Or see this region's nucleotide sequence