Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24155 and RR42_RS24160 are separated by 68 nucleotides RR42_RS24160 and RR42_RS24165 are separated by 60 nucleotides
RR42_RS24155: RR42_RS24155 - monooxygenase, at 745,352 to 746,494
_RS24155
RR42_RS24160: RR42_RS24160 - hypothetical protein, at 746,563 to 747,075
_RS24160
RR42_RS24165: RR42_RS24165 - gentisate 1,2-dioxygenase, at 747,136 to 748,164
_RS24165
Position (kb)
746
747
748 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 745.659 kb on + strand, within RR42_RS24155 at 745.681 kb on - strand, within RR42_RS24155 at 745.689 kb on + strand, within RR42_RS24155 at 745.689 kb on + strand, within RR42_RS24155 at 745.817 kb on + strand, within RR42_RS24155 at 745.817 kb on + strand, within RR42_RS24155 at 745.882 kb on + strand, within RR42_RS24155 at 745.882 kb on + strand, within RR42_RS24155 at 745.882 kb on + strand, within RR42_RS24155 at 745.883 kb on - strand, within RR42_RS24155 at 745.891 kb on - strand, within RR42_RS24155 at 745.920 kb on - strand, within RR42_RS24155 at 746.209 kb on - strand, within RR42_RS24155 at 746.209 kb on - strand, within RR42_RS24155 at 746.209 kb on - strand, within RR42_RS24155 at 746.391 kb on - strand at 746.451 kb on + strand at 746.781 kb on + strand, within RR42_RS24160 at 746.803 kb on - strand, within RR42_RS24160 at 746.890 kb on + strand, within RR42_RS24160 at 746.890 kb on + strand, within RR42_RS24160 at 746.891 kb on - strand, within RR42_RS24160 at 746.968 kb on + strand, within RR42_RS24160 at 747.004 kb on + strand, within RR42_RS24160 at 747.012 kb on + strand, within RR42_RS24160 at 747.023 kb on + strand, within RR42_RS24160 at 747.073 kb on + strand at 747.073 kb on + strand at 747.073 kb on + strand at 747.073 kb on + strand at 747.073 kb on + strand at 747.074 kb on - strand at 747.074 kb on - strand at 747.281 kb on - strand, within RR42_RS24165 at 747.410 kb on + strand, within RR42_RS24165 at 747.410 kb on + strand, within RR42_RS24165 at 747.490 kb on + strand, within RR42_RS24165 at 747.491 kb on - strand, within RR42_RS24165 at 747.491 kb on - strand, within RR42_RS24165 at 747.543 kb on + strand, within RR42_RS24165 at 747.544 kb on - strand, within RR42_RS24165 at 747.596 kb on + strand, within RR42_RS24165 at 747.597 kb on - strand, within RR42_RS24165 at 747.776 kb on - strand, within RR42_RS24165 at 747.859 kb on + strand, within RR42_RS24165 at 747.859 kb on + strand, within RR42_RS24165 at 747.860 kb on - strand, within RR42_RS24165 at 747.860 kb on - strand, within RR42_RS24165 at 747.860 kb on - strand, within RR42_RS24165 at 747.860 kb on - strand, within RR42_RS24165 at 747.911 kb on + strand, within RR42_RS24165 at 747.911 kb on + strand, within RR42_RS24165 at 747.911 kb on + strand, within RR42_RS24165 at 747.912 kb on - strand, within RR42_RS24165 at 747.912 kb on - strand, within RR42_RS24165 at 747.917 kb on - strand, within RR42_RS24165 at 747.917 kb on - strand, within RR42_RS24165 at 747.954 kb on + strand, within RR42_RS24165 at 748.017 kb on + strand, within RR42_RS24165 at 748.018 kb on - strand, within RR42_RS24165 at 748.074 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 745,659 + RR42_RS24155 0.27 +0.3 745,681 - RR42_RS24155 0.29 +0.5 745,689 + RR42_RS24155 0.29 +0.3 745,689 + RR42_RS24155 0.29 -0.2 745,817 + RR42_RS24155 0.41 +0.3 745,817 + RR42_RS24155 0.41 +0.2 745,882 + RR42_RS24155 0.46 +1.2 745,882 + RR42_RS24155 0.46 -0.7 745,882 + RR42_RS24155 0.46 +0.8 745,883 - RR42_RS24155 0.46 -0.1 745,891 - RR42_RS24155 0.47 -0.3 745,920 - RR42_RS24155 0.50 +1.2 746,209 - RR42_RS24155 0.75 +0.5 746,209 - RR42_RS24155 0.75 +0.4 746,209 - RR42_RS24155 0.75 +1.5 746,391 - +0.5 746,451 + +0.6 746,781 + RR42_RS24160 0.42 +0.3 746,803 - RR42_RS24160 0.47 +0.4 746,890 + RR42_RS24160 0.64 -0.6 746,890 + RR42_RS24160 0.64 -0.4 746,891 - RR42_RS24160 0.64 -0.2 746,968 + RR42_RS24160 0.79 -1.5 747,004 + RR42_RS24160 0.86 +1.3 747,012 + RR42_RS24160 0.88 -0.8 747,023 + RR42_RS24160 0.90 +0.3 747,073 + +0.5 747,073 + -0.5 747,073 + -0.5 747,073 + -0.6 747,073 + -0.4 747,074 - +1.3 747,074 - +0.9 747,281 - RR42_RS24165 0.14 +0.2 747,410 + RR42_RS24165 0.27 -1.3 747,410 + RR42_RS24165 0.27 -0.3 747,490 + RR42_RS24165 0.34 -0.5 747,491 - RR42_RS24165 0.34 -0.6 747,491 - RR42_RS24165 0.34 -0.4 747,543 + RR42_RS24165 0.40 +0.4 747,544 - RR42_RS24165 0.40 +0.4 747,596 + RR42_RS24165 0.45 -0.0 747,597 - RR42_RS24165 0.45 -0.6 747,776 - RR42_RS24165 0.62 -1.0 747,859 + RR42_RS24165 0.70 +0.4 747,859 + RR42_RS24165 0.70 +0.9 747,860 - RR42_RS24165 0.70 +0.7 747,860 - RR42_RS24165 0.70 +0.3 747,860 - RR42_RS24165 0.70 -0.7 747,860 - RR42_RS24165 0.70 -2.1 747,911 + RR42_RS24165 0.75 +0.2 747,911 + RR42_RS24165 0.75 +0.3 747,911 + RR42_RS24165 0.75 +2.1 747,912 - RR42_RS24165 0.75 +0.2 747,912 - RR42_RS24165 0.75 +0.0 747,917 - RR42_RS24165 0.76 +0.6 747,917 - RR42_RS24165 0.76 -0.7 747,954 + RR42_RS24165 0.79 +2.7 748,017 + RR42_RS24165 0.86 +0.1 748,018 - RR42_RS24165 0.86 +0.2 748,074 + -1.1
Or see this region's nucleotide sequence