Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23755 and RR42_RS23760 are separated by 140 nucleotides RR42_RS23760 and RR42_RS23765 are separated by 4 nucleotides RR42_RS23765 and RR42_RS23770 are separated by 104 nucleotides RR42_RS23770 and RR42_RS23775 are separated by 647 nucleotides
RR42_RS23755: RR42_RS23755 - phenylacetate--CoA ligase, at 654,283 to 655,608
_RS23755
RR42_RS23760: RR42_RS23760 - tRNA-Asn, at 655,749 to 655,824
_RS23760
RR42_RS23765: RR42_RS23765 - tRNA-Gly, at 655,829 to 655,904
_RS23765
RR42_RS23770: RR42_RS23770 - hypothetical protein, at 656,009 to 656,920
_RS23770
RR42_RS23775: RR42_RS23775 - hypothetical protein, at 657,568 to 658,818
_RS23775
Position (kb)
656
657 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 655.158 kb on + strand, within RR42_RS23755 at 655.159 kb on - strand, within RR42_RS23755 at 655.363 kb on + strand, within RR42_RS23755 at 655.363 kb on + strand, within RR42_RS23755 at 655.364 kb on - strand, within RR42_RS23755 at 655.392 kb on + strand, within RR42_RS23755 at 655.756 kb on + strand at 655.768 kb on + strand, within RR42_RS23760 at 655.784 kb on + strand, within RR42_RS23760 at 655.784 kb on + strand, within RR42_RS23760 at 655.837 kb on - strand, within RR42_RS23765 at 655.837 kb on - strand, within RR42_RS23765 at 655.849 kb on - strand, within RR42_RS23765 at 655.849 kb on - strand, within RR42_RS23765 at 655.925 kb on + strand at 655.925 kb on + strand at 655.926 kb on - strand at 655.931 kb on - strand at 656.010 kb on + strand at 656.011 kb on - strand at 656.017 kb on - strand at 656.017 kb on - strand at 656.058 kb on + strand at 656.058 kb on + strand at 656.058 kb on + strand at 656.058 kb on + strand at 656.058 kb on + strand at 656.071 kb on + strand at 656.083 kb on + strand at 656.083 kb on + strand at 656.083 kb on + strand at 656.083 kb on + strand at 656.084 kb on - strand at 656.084 kb on - strand at 656.149 kb on + strand, within RR42_RS23770 at 656.149 kb on + strand, within RR42_RS23770 at 656.177 kb on + strand, within RR42_RS23770 at 656.177 kb on + strand, within RR42_RS23770 at 656.178 kb on - strand, within RR42_RS23770 at 656.178 kb on - strand, within RR42_RS23770 at 656.178 kb on - strand, within RR42_RS23770 at 656.188 kb on + strand, within RR42_RS23770 at 656.189 kb on - strand, within RR42_RS23770 at 656.189 kb on - strand, within RR42_RS23770 at 656.189 kb on - strand, within RR42_RS23770 at 656.328 kb on + strand, within RR42_RS23770 at 656.330 kb on + strand, within RR42_RS23770 at 656.331 kb on - strand, within RR42_RS23770 at 656.341 kb on - strand, within RR42_RS23770 at 656.425 kb on + strand, within RR42_RS23770 at 656.425 kb on + strand, within RR42_RS23770 at 656.426 kb on - strand, within RR42_RS23770 at 656.426 kb on - strand, within RR42_RS23770 at 656.426 kb on - strand, within RR42_RS23770 at 656.444 kb on + strand, within RR42_RS23770 at 656.444 kb on + strand, within RR42_RS23770 at 656.445 kb on - strand, within RR42_RS23770 at 656.497 kb on - strand, within RR42_RS23770 at 656.530 kb on + strand, within RR42_RS23770 at 656.531 kb on - strand, within RR42_RS23770 at 656.531 kb on - strand, within RR42_RS23770 at 656.531 kb on - strand, within RR42_RS23770 at 656.573 kb on + strand, within RR42_RS23770 at 656.574 kb on - strand, within RR42_RS23770 at 656.583 kb on - strand, within RR42_RS23770 at 656.720 kb on + strand, within RR42_RS23770 at 656.726 kb on - strand, within RR42_RS23770 at 656.730 kb on + strand, within RR42_RS23770 at 656.730 kb on + strand, within RR42_RS23770 at 656.731 kb on - strand, within RR42_RS23770 at 656.731 kb on - strand, within RR42_RS23770 at 656.731 kb on - strand, within RR42_RS23770 at 656.777 kb on - strand, within RR42_RS23770 at 656.810 kb on - strand, within RR42_RS23770 at 656.836 kb on + strand at 657.046 kb on + strand at 657.047 kb on - strand at 657.083 kb on + strand at 657.180 kb on + strand at 657.180 kb on + strand at 657.256 kb on + strand at 657.325 kb on + strand at 657.325 kb on + strand at 657.326 kb on - strand at 657.365 kb on + strand at 657.404 kb on - strand at 657.537 kb on + strand at 657.537 kb on + strand at 657.538 kb on - strand at 657.540 kb on - strand at 657.540 kb on - strand at 657.573 kb on + strand at 657.574 kb on - strand at 657.574 kb on - strand at 657.574 kb on - strand at 657.685 kb on + strand at 657.685 kb on + strand at 657.685 kb on + strand at 657.685 kb on + strand at 657.686 kb on - strand at 657.686 kb on - strand at 657.686 kb on - strand at 657.686 kb on - strand at 657.788 kb on + strand, within RR42_RS23775 at 657.788 kb on + strand, within RR42_RS23775 at 657.788 kb on + strand, within RR42_RS23775 at 657.789 kb on - strand, within RR42_RS23775 at 657.817 kb on + strand, within RR42_RS23775 at 657.817 kb on + strand, within RR42_RS23775 at 657.817 kb on + strand, within RR42_RS23775 at 657.817 kb on + strand, within RR42_RS23775 at 657.817 kb on + strand, within RR42_RS23775 at 657.817 kb on + strand, within RR42_RS23775 at 657.818 kb on - strand, within RR42_RS23775 at 657.827 kb on - strand, within RR42_RS23775 at 657.827 kb on - strand, within RR42_RS23775 at 657.833 kb on + strand, within RR42_RS23775 at 657.833 kb on + strand, within RR42_RS23775 at 657.834 kb on - strand, within RR42_RS23775 at 657.838 kb on - strand, within RR42_RS23775 at 657.865 kb on + strand, within RR42_RS23775 at 657.866 kb on - strand, within RR42_RS23775 at 657.866 kb on - strand, within RR42_RS23775 at 657.871 kb on + strand, within RR42_RS23775 at 657.871 kb on + strand, within RR42_RS23775 at 657.871 kb on + strand, within RR42_RS23775 at 657.872 kb on - strand, within RR42_RS23775 at 657.872 kb on - strand, within RR42_RS23775 at 657.872 kb on - strand, within RR42_RS23775 at 657.872 kb on - strand, within RR42_RS23775 at 657.872 kb on - strand, within RR42_RS23775 at 657.900 kb on - strand, within RR42_RS23775
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 655,158 + RR42_RS23755 0.66 +1.8 655,159 - RR42_RS23755 0.66 -0.0 655,363 + RR42_RS23755 0.81 -2.0 655,363 + RR42_RS23755 0.81 +0.0 655,364 - RR42_RS23755 0.82 +0.7 655,392 + RR42_RS23755 0.84 -0.2 655,756 + -2.0 655,768 + RR42_RS23760 0.25 -1.0 655,784 + RR42_RS23760 0.46 -1.5 655,784 + RR42_RS23760 0.46 -0.9 655,837 - RR42_RS23765 0.11 +0.4 655,837 - RR42_RS23765 0.11 -0.2 655,849 - RR42_RS23765 0.26 +0.9 655,849 - RR42_RS23765 0.26 +1.9 655,925 + +0.0 655,925 + +0.6 655,926 - -0.6 655,931 - -0.4 656,010 + -0.9 656,011 - -0.6 656,017 - +0.5 656,017 - +0.0 656,058 + +2.0 656,058 + -2.4 656,058 + -0.9 656,058 + -0.4 656,058 + +0.4 656,071 + +0.4 656,083 + +0.2 656,083 + -0.1 656,083 + -1.4 656,083 + +0.2 656,084 - -0.7 656,084 - -0.8 656,149 + RR42_RS23770 0.15 +0.3 656,149 + RR42_RS23770 0.15 -0.2 656,177 + RR42_RS23770 0.18 -1.0 656,177 + RR42_RS23770 0.18 -0.3 656,178 - RR42_RS23770 0.19 +0.4 656,178 - RR42_RS23770 0.19 +0.3 656,178 - RR42_RS23770 0.19 +0.2 656,188 + RR42_RS23770 0.20 -0.4 656,189 - RR42_RS23770 0.20 -1.8 656,189 - RR42_RS23770 0.20 +0.9 656,189 - RR42_RS23770 0.20 +0.3 656,328 + RR42_RS23770 0.35 +0.1 656,330 + RR42_RS23770 0.35 -2.2 656,331 - RR42_RS23770 0.35 +0.5 656,341 - RR42_RS23770 0.36 +0.4 656,425 + RR42_RS23770 0.46 -0.7 656,425 + RR42_RS23770 0.46 -0.5 656,426 - RR42_RS23770 0.46 -1.4 656,426 - RR42_RS23770 0.46 +0.8 656,426 - RR42_RS23770 0.46 +0.7 656,444 + RR42_RS23770 0.48 -0.8 656,444 + RR42_RS23770 0.48 -1.4 656,445 - RR42_RS23770 0.48 -0.3 656,497 - RR42_RS23770 0.54 +0.6 656,530 + RR42_RS23770 0.57 -0.1 656,531 - RR42_RS23770 0.57 -0.1 656,531 - RR42_RS23770 0.57 +0.6 656,531 - RR42_RS23770 0.57 +0.1 656,573 + RR42_RS23770 0.62 -1.2 656,574 - RR42_RS23770 0.62 -0.5 656,583 - RR42_RS23770 0.63 -0.2 656,720 + RR42_RS23770 0.78 -0.5 656,726 - RR42_RS23770 0.79 -0.8 656,730 + RR42_RS23770 0.79 -0.9 656,730 + RR42_RS23770 0.79 -1.6 656,731 - RR42_RS23770 0.79 -1.0 656,731 - RR42_RS23770 0.79 +0.2 656,731 - RR42_RS23770 0.79 -0.3 656,777 - RR42_RS23770 0.84 +1.0 656,810 - RR42_RS23770 0.88 +0.7 656,836 + +0.1 657,046 + -0.6 657,047 - +0.2 657,083 + -0.1 657,180 + +0.7 657,180 + -1.4 657,256 + +0.7 657,325 + +0.7 657,325 + -1.4 657,326 - -1.7 657,365 + +0.4 657,404 - -0.6 657,537 + -0.1 657,537 + -0.2 657,538 - +1.0 657,540 - -0.5 657,540 - -0.8 657,573 + +0.1 657,574 - -0.8 657,574 - -1.3 657,574 - +1.0 657,685 + -0.3 657,685 + -1.1 657,685 + -2.3 657,685 + +0.4 657,686 - +0.7 657,686 - -1.2 657,686 - +0.3 657,686 - +0.2 657,788 + RR42_RS23775 0.18 +0.8 657,788 + RR42_RS23775 0.18 +0.2 657,788 + RR42_RS23775 0.18 -0.1 657,789 - RR42_RS23775 0.18 -0.1 657,817 + RR42_RS23775 0.20 -0.9 657,817 + RR42_RS23775 0.20 +0.4 657,817 + RR42_RS23775 0.20 +0.1 657,817 + RR42_RS23775 0.20 -0.1 657,817 + RR42_RS23775 0.20 -0.4 657,817 + RR42_RS23775 0.20 -0.8 657,818 - RR42_RS23775 0.20 +0.2 657,827 - RR42_RS23775 0.21 +0.4 657,827 - RR42_RS23775 0.21 -0.0 657,833 + RR42_RS23775 0.21 -2.0 657,833 + RR42_RS23775 0.21 +0.1 657,834 - RR42_RS23775 0.21 -0.0 657,838 - RR42_RS23775 0.22 -1.3 657,865 + RR42_RS23775 0.24 +1.7 657,866 - RR42_RS23775 0.24 +0.8 657,866 - RR42_RS23775 0.24 -0.8 657,871 + RR42_RS23775 0.24 -0.0 657,871 + RR42_RS23775 0.24 +0.1 657,871 + RR42_RS23775 0.24 -0.5 657,872 - RR42_RS23775 0.24 -0.9 657,872 - RR42_RS23775 0.24 -0.5 657,872 - RR42_RS23775 0.24 +0.6 657,872 - RR42_RS23775 0.24 -2.0 657,872 - RR42_RS23775 0.24 -0.5 657,900 - RR42_RS23775 0.27 -0.1
Or see this region's nucleotide sequence