Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22930 and RR42_RS22935 are separated by 53 nucleotides RR42_RS22935 and RR42_RS22940 are separated by 60 nucleotides RR42_RS22940 and RR42_RS22945 are separated by 137 nucleotides
RR42_RS22930: RR42_RS22930 - cytochrome C peroxidase, at 476,193 to 479,342
_RS22930
RR42_RS22935: RR42_RS22935 - metal transporter, at 479,396 to 480,613
_RS22935
RR42_RS22940: RR42_RS22940 - TetR family transcriptional regulator, at 480,674 to 481,348
_RS22940
RR42_RS22945: RR42_RS22945 - oxidoreductase, at 481,486 to 482,514
_RS22945
Position (kb) 479
480
481 Strain fitness (log2 ratio) -2
-1
0
1 at 478.500 kb on - strand, within RR42_RS22930 at 478.886 kb on + strand, within RR42_RS22930 at 478.886 kb on + strand, within RR42_RS22930 at 478.887 kb on - strand, within RR42_RS22930 at 478.938 kb on + strand, within RR42_RS22930 at 479.024 kb on + strand, within RR42_RS22930 at 479.025 kb on - strand, within RR42_RS22930 at 479.866 kb on - strand, within RR42_RS22935 at 480.080 kb on + strand, within RR42_RS22935 at 480.080 kb on + strand, within RR42_RS22935 at 480.081 kb on - strand, within RR42_RS22935 at 480.458 kb on + strand, within RR42_RS22935 at 480.661 kb on + strand at 480.819 kb on - strand, within RR42_RS22940 at 481.130 kb on - strand, within RR42_RS22940 at 481.182 kb on + strand, within RR42_RS22940 at 481.183 kb on - strand, within RR42_RS22940
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 478,500 - RR42_RS22930 0.73 -0.8 478,886 + RR42_RS22930 0.85 +0.1 478,886 + RR42_RS22930 0.85 -0.0 478,887 - RR42_RS22930 0.86 -2.6 478,938 + RR42_RS22930 0.87 -0.7 479,024 + RR42_RS22930 0.90 -2.0 479,025 - RR42_RS22930 0.90 -1.4 479,866 - RR42_RS22935 0.39 -0.5 480,080 + RR42_RS22935 0.56 -0.3 480,080 + RR42_RS22935 0.56 -0.8 480,081 - RR42_RS22935 0.56 -1.0 480,458 + RR42_RS22935 0.87 -1.4 480,661 + -0.1 480,819 - RR42_RS22940 0.21 -1.5 481,130 - RR42_RS22940 0.68 -1.2 481,182 + RR42_RS22940 0.75 +0.7 481,183 - RR42_RS22940 0.75 -1.5
Or see this region's nucleotide sequence