Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22725 and RR42_RS22730 are separated by 480 nucleotides RR42_RS22730 and RR42_RS22735 are separated by 20 nucleotides RR42_RS22735 and RR42_RS22740 are separated by 121 nucleotides
RR42_RS22725: RR42_RS22725 - phosphatidylethanolamine-binding protein, at 432,778 to 433,332
_RS22725
RR42_RS22730: RR42_RS22730 - pyridine nucleotide-disulfide oxidoreductase, at 433,813 to 435,018
_RS22730
RR42_RS22735: RR42_RS22735 - transcriptional regulator, at 435,039 to 435,578
_RS22735
RR42_RS22740: RR42_RS22740 - oxidoreductase, at 435,700 to 436,494
_RS22740
Position (kb) 433
434
435
436 Strain fitness (log2 ratio) -1
0
1
2 at 432.847 kb on + strand, within RR42_RS22725 at 432.847 kb on + strand, within RR42_RS22725 at 432.847 kb on + strand, within RR42_RS22725 at 432.847 kb on + strand, within RR42_RS22725 at 432.910 kb on + strand, within RR42_RS22725 at 432.910 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.922 kb on - strand, within RR42_RS22725 at 432.922 kb on - strand, within RR42_RS22725 at 432.922 kb on - strand, within RR42_RS22725 at 433.034 kb on + strand, within RR42_RS22725 at 433.039 kb on + strand, within RR42_RS22725 at 433.057 kb on + strand, within RR42_RS22725 at 433.059 kb on + strand, within RR42_RS22725 at 433.060 kb on - strand, within RR42_RS22725 at 433.139 kb on + strand, within RR42_RS22725 at 433.198 kb on + strand, within RR42_RS22725 at 433.198 kb on + strand, within RR42_RS22725 at 433.199 kb on - strand, within RR42_RS22725 at 433.199 kb on - strand, within RR42_RS22725 at 433.322 kb on - strand at 433.342 kb on + strand at 433.343 kb on - strand at 433.433 kb on + strand at 433.434 kb on - strand at 433.793 kb on - strand at 433.819 kb on - strand at 433.825 kb on - strand at 433.963 kb on + strand, within RR42_RS22730 at 434.116 kb on + strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.136 kb on + strand, within RR42_RS22730 at 434.233 kb on - strand, within RR42_RS22730 at 434.233 kb on - strand, within RR42_RS22730 at 434.274 kb on + strand, within RR42_RS22730 at 434.275 kb on - strand, within RR42_RS22730 at 434.693 kb on - strand, within RR42_RS22730 at 434.693 kb on - strand, within RR42_RS22730 at 434.719 kb on + strand, within RR42_RS22730 at 434.719 kb on + strand, within RR42_RS22730 at 434.719 kb on + strand, within RR42_RS22730 at 434.720 kb on - strand, within RR42_RS22730 at 434.720 kb on - strand, within RR42_RS22730 at 435.011 kb on + strand at 435.016 kb on + strand at 435.105 kb on + strand, within RR42_RS22735 at 435.105 kb on + strand, within RR42_RS22735 at 435.106 kb on - strand, within RR42_RS22735 at 435.106 kb on - strand, within RR42_RS22735 at 435.452 kb on - strand, within RR42_RS22735 at 435.495 kb on - strand, within RR42_RS22735 at 435.605 kb on - strand at 435.615 kb on - strand at 435.617 kb on - strand at 435.712 kb on - strand at 435.780 kb on - strand, within RR42_RS22740 at 435.780 kb on - strand, within RR42_RS22740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 432,847 + RR42_RS22725 0.12 +0.3 432,847 + RR42_RS22725 0.12 -0.1 432,847 + RR42_RS22725 0.12 -0.4 432,847 + RR42_RS22725 0.12 +0.0 432,910 + RR42_RS22725 0.24 +0.8 432,910 + RR42_RS22725 0.24 +0.1 432,921 + RR42_RS22725 0.26 +1.1 432,921 + RR42_RS22725 0.26 +0.3 432,921 + RR42_RS22725 0.26 +0.4 432,921 + RR42_RS22725 0.26 +0.1 432,922 - RR42_RS22725 0.26 -0.4 432,922 - RR42_RS22725 0.26 -1.5 432,922 - RR42_RS22725 0.26 +0.1 433,034 + RR42_RS22725 0.46 +0.1 433,039 + RR42_RS22725 0.47 +0.5 433,057 + RR42_RS22725 0.50 -0.9 433,059 + RR42_RS22725 0.51 +0.4 433,060 - RR42_RS22725 0.51 -0.6 433,139 + RR42_RS22725 0.65 +0.1 433,198 + RR42_RS22725 0.76 -0.3 433,198 + RR42_RS22725 0.76 -0.5 433,199 - RR42_RS22725 0.76 -0.6 433,199 - RR42_RS22725 0.76 +0.2 433,322 - +0.9 433,342 + -0.9 433,343 - -0.4 433,433 + -1.0 433,434 - -0.2 433,793 - +1.3 433,819 - -0.6 433,825 - -0.1 433,963 + RR42_RS22730 0.12 +0.7 434,116 + RR42_RS22730 0.25 -0.5 434,117 - RR42_RS22730 0.25 +0.5 434,117 - RR42_RS22730 0.25 +0.4 434,117 - RR42_RS22730 0.25 +0.5 434,117 - RR42_RS22730 0.25 +0.5 434,136 + RR42_RS22730 0.27 +0.5 434,233 - RR42_RS22730 0.35 +0.3 434,233 - RR42_RS22730 0.35 +1.0 434,274 + RR42_RS22730 0.38 -0.2 434,275 - RR42_RS22730 0.38 -0.0 434,693 - RR42_RS22730 0.73 +1.0 434,693 - RR42_RS22730 0.73 +0.9 434,719 + RR42_RS22730 0.75 -0.2 434,719 + RR42_RS22730 0.75 +0.2 434,719 + RR42_RS22730 0.75 +0.5 434,720 - RR42_RS22730 0.75 +1.8 434,720 - RR42_RS22730 0.75 -0.5 435,011 + -0.1 435,016 + -0.4 435,105 + RR42_RS22735 0.12 -0.6 435,105 + RR42_RS22735 0.12 +0.2 435,106 - RR42_RS22735 0.12 -0.3 435,106 - RR42_RS22735 0.12 -0.3 435,452 - RR42_RS22735 0.76 -0.4 435,495 - RR42_RS22735 0.84 +0.0 435,605 - +0.0 435,615 - +0.4 435,617 - +0.5 435,712 - +0.5 435,780 - RR42_RS22740 0.10 -0.0 435,780 - RR42_RS22740 0.10 +0.5
Or see this region's nucleotide sequence