Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22015 and RR42_RS22020 are separated by 15 nucleotides RR42_RS22020 and RR42_RS22025 are separated by 256 nucleotides RR42_RS22025 and RR42_RS22030 are separated by 164 nucleotides RR42_RS22030 and RR42_RS22035 overlap by 4 nucleotides
RR42_RS22015: RR42_RS22015 - histidine kinase, at 271,259 to 273,655
_RS22015
RR42_RS22020: RR42_RS22020 - hypothetical protein, at 273,671 to 273,856
_RS22020
RR42_RS22025: RR42_RS22025 - AraC family transcriptional regulator, at 274,113 to 275,090
_RS22025
RR42_RS22030: RR42_RS22030 - hypothetical protein, at 275,255 to 275,689
_RS22030
RR42_RS22035: RR42_RS22035 - ATPase, at 275,686 to 278,376
_RS22035
Position (kb) 274
275
276 Strain fitness (log2 ratio) -2
-1
0
1 at 273.244 kb on - strand, within RR42_RS22015 at 273.244 kb on - strand, within RR42_RS22015 at 273.414 kb on - strand, within RR42_RS22015 at 273.846 kb on + strand at 273.847 kb on - strand at 273.902 kb on + strand at 273.916 kb on - strand at 273.936 kb on - strand at 274.017 kb on + strand at 274.018 kb on - strand at 274.018 kb on - strand at 274.162 kb on + strand at 274.275 kb on - strand, within RR42_RS22025 at 274.275 kb on - strand, within RR42_RS22025 at 274.279 kb on - strand, within RR42_RS22025 at 274.654 kb on + strand, within RR42_RS22025 at 274.654 kb on + strand, within RR42_RS22025 at 274.655 kb on - strand, within RR42_RS22025 at 274.655 kb on - strand, within RR42_RS22025 at 274.788 kb on + strand, within RR42_RS22025 at 274.788 kb on + strand, within RR42_RS22025 at 274.875 kb on + strand, within RR42_RS22025 at 275.116 kb on + strand at 275.434 kb on - strand, within RR42_RS22030 at 275.453 kb on + strand, within RR42_RS22030 at 275.454 kb on - strand, within RR42_RS22030 at 275.496 kb on + strand, within RR42_RS22030 at 275.496 kb on + strand, within RR42_RS22030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 273,244 - RR42_RS22015 0.83 +0.6 273,244 - RR42_RS22015 0.83 -1.2 273,414 - RR42_RS22015 0.90 -2.4 273,846 + +0.9 273,847 - +0.4 273,902 + -0.7 273,916 - +0.9 273,936 - +1.6 274,017 + -0.2 274,018 - +0.6 274,018 - -0.4 274,162 + -0.3 274,275 - RR42_RS22025 0.17 +0.8 274,275 - RR42_RS22025 0.17 +0.4 274,279 - RR42_RS22025 0.17 +0.3 274,654 + RR42_RS22025 0.55 -0.2 274,654 + RR42_RS22025 0.55 +0.7 274,655 - RR42_RS22025 0.55 +0.5 274,655 - RR42_RS22025 0.55 -0.2 274,788 + RR42_RS22025 0.69 +1.2 274,788 + RR42_RS22025 0.69 +0.2 274,875 + RR42_RS22025 0.78 -0.5 275,116 + +1.6 275,434 - RR42_RS22030 0.41 -0.7 275,453 + RR42_RS22030 0.46 -0.4 275,454 - RR42_RS22030 0.46 -0.4 275,496 + RR42_RS22030 0.55 -0.4 275,496 + RR42_RS22030 0.55 -1.5
Or see this region's nucleotide sequence