Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21975 and RR42_RS21980 are separated by 59 nucleotides RR42_RS21980 and RR42_RS21985 are separated by 13 nucleotides RR42_RS21985 and RR42_RS21990 overlap by 8 nucleotides RR42_RS21990 and RR42_RS21995 overlap by 4 nucleotides
RR42_RS21975: RR42_RS21975 - hypothetical protein, at 258,302 to 262,522
_RS21975
RR42_RS21980: RR42_RS21980 - transporter, at 262,582 to 263,139
_RS21980
RR42_RS21985: RR42_RS21985 - sugar ABC transporter, at 263,153 to 264,598
_RS21985
RR42_RS21990: RR42_RS21990 - LPS N-acetylglucosaminyl transferase, at 264,591 to 265,577
_RS21990
RR42_RS21995: RR42_RS21995 - glycosyl transferase family 1, at 265,574 to 267,175
_RS21995
Position (kb) 263
264
265 Strain fitness (log2 ratio) -1
0
1 at 262.176 kb on - strand at 262.253 kb on + strand at 262.253 kb on + strand at 262.254 kb on - strand at 262.254 kb on - strand at 262.320 kb on - strand at 262.320 kb on - strand at 262.320 kb on - strand at 262.442 kb on + strand at 262.442 kb on + strand at 262.442 kb on + strand at 262.443 kb on - strand at 262.443 kb on - strand at 262.556 kb on + strand at 262.557 kb on - strand at 262.593 kb on + strand at 262.593 kb on + strand at 262.593 kb on + strand at 262.744 kb on + strand, within RR42_RS21980 at 262.744 kb on + strand, within RR42_RS21980 at 262.745 kb on - strand, within RR42_RS21980 at 262.961 kb on - strand, within RR42_RS21980 at 263.168 kb on - strand at 263.304 kb on - strand, within RR42_RS21985 at 263.978 kb on + strand, within RR42_RS21985 at 263.978 kb on + strand, within RR42_RS21985 at 264.066 kb on - strand, within RR42_RS21985 at 264.104 kb on + strand, within RR42_RS21985 at 264.398 kb on + strand, within RR42_RS21985 at 264.472 kb on + strand at 264.472 kb on + strand at 264.472 kb on + strand at 264.577 kb on - strand at 264.597 kb on - strand at 264.777 kb on + strand, within RR42_RS21990 at 264.778 kb on - strand, within RR42_RS21990 at 264.778 kb on - strand, within RR42_RS21990 at 264.778 kb on - strand, within RR42_RS21990 at 264.783 kb on + strand, within RR42_RS21990 at 264.793 kb on + strand, within RR42_RS21990 at 264.793 kb on + strand, within RR42_RS21990 at 264.793 kb on + strand, within RR42_RS21990 at 265.107 kb on + strand, within RR42_RS21990 at 265.245 kb on + strand, within RR42_RS21990 at 265.246 kb on - strand, within RR42_RS21990 at 265.273 kb on - strand, within RR42_RS21990 at 265.350 kb on + strand, within RR42_RS21990 at 265.351 kb on - strand, within RR42_RS21990 at 265.351 kb on - strand, within RR42_RS21990 at 265.351 kb on - strand, within RR42_RS21990 at 265.563 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 262,176 - -0.1 262,253 + +0.3 262,253 + -0.1 262,254 - +0.9 262,254 - -0.6 262,320 - +0.2 262,320 - +0.5 262,320 - -0.0 262,442 + +0.6 262,442 + +0.7 262,442 + -1.0 262,443 - +0.0 262,443 - +0.3 262,556 + +0.6 262,557 - -0.0 262,593 + -0.5 262,593 + +0.5 262,593 + +1.1 262,744 + RR42_RS21980 0.29 +0.8 262,744 + RR42_RS21980 0.29 -0.0 262,745 - RR42_RS21980 0.29 +0.2 262,961 - RR42_RS21980 0.68 -0.4 263,168 - +0.5 263,304 - RR42_RS21985 0.10 +1.2 263,978 + RR42_RS21985 0.57 +0.4 263,978 + RR42_RS21985 0.57 +0.4 264,066 - RR42_RS21985 0.63 -0.5 264,104 + RR42_RS21985 0.66 -0.1 264,398 + RR42_RS21985 0.86 +0.3 264,472 + +0.1 264,472 + -1.2 264,472 + +0.1 264,577 - -0.8 264,597 - -0.2 264,777 + RR42_RS21990 0.19 +0.6 264,778 - RR42_RS21990 0.19 +0.2 264,778 - RR42_RS21990 0.19 +0.3 264,778 - RR42_RS21990 0.19 +0.1 264,783 + RR42_RS21990 0.19 -0.9 264,793 + RR42_RS21990 0.20 +0.8 264,793 + RR42_RS21990 0.20 -1.6 264,793 + RR42_RS21990 0.20 -0.2 265,107 + RR42_RS21990 0.52 +0.2 265,245 + RR42_RS21990 0.66 -0.8 265,246 - RR42_RS21990 0.66 -0.4 265,273 - RR42_RS21990 0.69 -0.8 265,350 + RR42_RS21990 0.77 -0.1 265,351 - RR42_RS21990 0.77 -0.3 265,351 - RR42_RS21990 0.77 +0.8 265,351 - RR42_RS21990 0.77 -0.3 265,563 + -0.1
Or see this region's nucleotide sequence