Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS19910 and RR42_RS19915 are separated by 21 nucleotides RR42_RS19915 and RR42_RS19920 are separated by 44 nucleotides RR42_RS19920 and RR42_RS19925 are separated by 288 nucleotides
RR42_RS19910: RR42_RS19910 - membrane protein, at 4,299,702 to 4,301,222
_RS19910
RR42_RS19915: RR42_RS19915 - SPFH domain/Band 7 family protein 2, at 4,301,244 to 4,302,278
_RS19915
RR42_RS19920: RR42_RS19920 - membrane protein, at 4,302,323 to 4,302,709
_RS19920
RR42_RS19925: RR42_RS19925 - spermidine synthase, at 4,302,998 to 4,304,509
_RS19925
Position (kb)
4301
4302
4303 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4300.273 kb on + strand, within RR42_RS19910 at 4300.274 kb on - strand, within RR42_RS19910 at 4300.274 kb on - strand, within RR42_RS19910 at 4300.339 kb on + strand, within RR42_RS19910 at 4300.636 kb on + strand, within RR42_RS19910 at 4300.636 kb on + strand, within RR42_RS19910 at 4300.637 kb on - strand, within RR42_RS19910 at 4300.792 kb on + strand, within RR42_RS19910 at 4300.792 kb on + strand, within RR42_RS19910 at 4300.810 kb on + strand, within RR42_RS19910 at 4300.811 kb on - strand, within RR42_RS19910 at 4300.909 kb on + strand, within RR42_RS19910 at 4300.909 kb on + strand, within RR42_RS19910 at 4300.910 kb on - strand, within RR42_RS19910 at 4300.910 kb on - strand, within RR42_RS19910 at 4300.910 kb on - strand, within RR42_RS19910 at 4300.963 kb on + strand, within RR42_RS19910 at 4300.964 kb on - strand, within RR42_RS19910 at 4301.068 kb on + strand, within RR42_RS19910 at 4301.069 kb on - strand, within RR42_RS19910 at 4301.226 kb on + strand at 4301.234 kb on - strand at 4301.542 kb on + strand, within RR42_RS19915 at 4301.543 kb on - strand, within RR42_RS19915 at 4301.562 kb on - strand, within RR42_RS19915 at 4301.586 kb on - strand, within RR42_RS19915 at 4301.646 kb on - strand, within RR42_RS19915 at 4301.662 kb on + strand, within RR42_RS19915 at 4301.743 kb on - strand, within RR42_RS19915 at 4301.822 kb on - strand, within RR42_RS19915 at 4301.851 kb on + strand, within RR42_RS19915 at 4301.851 kb on + strand, within RR42_RS19915 at 4301.851 kb on + strand, within RR42_RS19915 at 4301.852 kb on - strand, within RR42_RS19915 at 4301.878 kb on + strand, within RR42_RS19915 at 4301.884 kb on + strand, within RR42_RS19915 at 4301.884 kb on + strand, within RR42_RS19915 at 4301.885 kb on - strand, within RR42_RS19915 at 4301.992 kb on + strand, within RR42_RS19915 at 4301.992 kb on + strand, within RR42_RS19915 at 4301.992 kb on + strand, within RR42_RS19915 at 4301.993 kb on - strand, within RR42_RS19915 at 4301.993 kb on - strand, within RR42_RS19915 at 4301.993 kb on - strand, within RR42_RS19915 at 4302.044 kb on - strand, within RR42_RS19915 at 4302.044 kb on - strand, within RR42_RS19915 at 4302.194 kb on - strand at 4302.208 kb on - strand at 4302.245 kb on + strand at 4302.246 kb on - strand at 4302.367 kb on + strand, within RR42_RS19920 at 4302.394 kb on + strand, within RR42_RS19920 at 4302.637 kb on - strand, within RR42_RS19920 at 4302.637 kb on - strand, within RR42_RS19920 at 4302.637 kb on - strand, within RR42_RS19920 at 4302.690 kb on + strand at 4302.690 kb on + strand at 4302.696 kb on + strand at 4302.696 kb on + strand at 4302.697 kb on - strand at 4302.697 kb on - strand at 4302.768 kb on + strand at 4302.768 kb on + strand at 4302.802 kb on - strand at 4302.840 kb on + strand at 4302.841 kb on - strand at 4302.841 kb on - strand at 4302.841 kb on - strand at 4302.842 kb on + strand at 4303.085 kb on + strand at 4303.085 kb on + strand at 4303.085 kb on + strand at 4303.086 kb on - strand at 4303.086 kb on - strand at 4303.086 kb on - strand at 4303.134 kb on - strand at 4303.173 kb on - strand, within RR42_RS19925
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 4,300,273 + RR42_RS19910 0.38 +0.2 4,300,274 - RR42_RS19910 0.38 -1.4 4,300,274 - RR42_RS19910 0.38 +0.2 4,300,339 + RR42_RS19910 0.42 -0.2 4,300,636 + RR42_RS19910 0.61 -2.4 4,300,636 + RR42_RS19910 0.61 +0.6 4,300,637 - RR42_RS19910 0.61 -1.7 4,300,792 + RR42_RS19910 0.72 +0.0 4,300,792 + RR42_RS19910 0.72 +0.5 4,300,810 + RR42_RS19910 0.73 -0.8 4,300,811 - RR42_RS19910 0.73 -0.3 4,300,909 + RR42_RS19910 0.79 -0.9 4,300,909 + RR42_RS19910 0.79 +0.1 4,300,910 - RR42_RS19910 0.79 -0.4 4,300,910 - RR42_RS19910 0.79 -0.7 4,300,910 - RR42_RS19910 0.79 +0.2 4,300,963 + RR42_RS19910 0.83 -0.1 4,300,964 - RR42_RS19910 0.83 +2.7 4,301,068 + RR42_RS19910 0.90 -1.5 4,301,069 - RR42_RS19910 0.90 -0.4 4,301,226 + -0.4 4,301,234 - +1.7 4,301,542 + RR42_RS19915 0.29 -1.1 4,301,543 - RR42_RS19915 0.29 -0.5 4,301,562 - RR42_RS19915 0.31 -0.8 4,301,586 - RR42_RS19915 0.33 -0.8 4,301,646 - RR42_RS19915 0.39 +1.2 4,301,662 + RR42_RS19915 0.40 -1.8 4,301,743 - RR42_RS19915 0.48 -0.6 4,301,822 - RR42_RS19915 0.56 +0.0 4,301,851 + RR42_RS19915 0.59 -0.0 4,301,851 + RR42_RS19915 0.59 -0.3 4,301,851 + RR42_RS19915 0.59 -0.4 4,301,852 - RR42_RS19915 0.59 +0.4 4,301,878 + RR42_RS19915 0.61 -0.2 4,301,884 + RR42_RS19915 0.62 -0.7 4,301,884 + RR42_RS19915 0.62 +0.0 4,301,885 - RR42_RS19915 0.62 -1.7 4,301,992 + RR42_RS19915 0.72 +0.6 4,301,992 + RR42_RS19915 0.72 +0.4 4,301,992 + RR42_RS19915 0.72 -0.4 4,301,993 - RR42_RS19915 0.72 +0.4 4,301,993 - RR42_RS19915 0.72 -0.3 4,301,993 - RR42_RS19915 0.72 +0.6 4,302,044 - RR42_RS19915 0.77 -0.4 4,302,044 - RR42_RS19915 0.77 -0.4 4,302,194 - -2.4 4,302,208 - -0.3 4,302,245 + -0.2 4,302,246 - -0.4 4,302,367 + RR42_RS19920 0.11 -1.7 4,302,394 + RR42_RS19920 0.18 -1.0 4,302,637 - RR42_RS19920 0.81 -1.2 4,302,637 - RR42_RS19920 0.81 -0.7 4,302,637 - RR42_RS19920 0.81 -0.9 4,302,690 + -0.3 4,302,690 + -0.0 4,302,696 + -0.5 4,302,696 + +0.0 4,302,697 - -0.2 4,302,697 - -0.2 4,302,768 + -0.7 4,302,768 + -0.6 4,302,802 - -0.4 4,302,840 + +0.3 4,302,841 - -0.6 4,302,841 - +0.4 4,302,841 - +0.7 4,302,842 + -0.3 4,303,085 + -0.9 4,303,085 + -0.1 4,303,085 + +0.7 4,303,086 - -0.7 4,303,086 - -2.2 4,303,086 - -1.6 4,303,134 - +0.3 4,303,173 - RR42_RS19925 0.12 +0.0
Or see this region's nucleotide sequence