Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18720 and RR42_RS18725 are separated by 113 nucleotides RR42_RS18725 and RR42_RS18730 are separated by 15 nucleotides RR42_RS18730 and RR42_RS18735 are separated by 44 nucleotides RR42_RS18735 and RR42_RS18740 are separated by 426 nucleotides
RR42_RS18720: RR42_RS18720 - hypothetical protein, at 4,056,312 to 4,057,247
_RS18720
RR42_RS18725: RR42_RS18725 - hypothetical protein, at 4,057,361 to 4,057,672
_RS18725
RR42_RS18730: RR42_RS18730 - hypothetical protein, at 4,057,688 to 4,057,990
_RS18730
RR42_RS18735: RR42_RS18735 - DNA-binding protein, at 4,058,035 to 4,058,403
_RS18735
RR42_RS18740: RR42_RS18740 - hypothetical protein, at 4,058,830 to 4,060,320
_RS18740
Position (kb)
4057
4058 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4057.163 kb on - strand at 4057.253 kb on + strand at 4057.254 kb on - strand at 4057.254 kb on - strand at 4057.254 kb on - strand at 4057.306 kb on + strand at 4057.306 kb on + strand at 4057.306 kb on + strand at 4057.307 kb on - strand at 4057.307 kb on - strand at 4057.586 kb on + strand, within RR42_RS18725 at 4057.586 kb on + strand, within RR42_RS18725 at 4057.587 kb on - strand, within RR42_RS18725 at 4057.910 kb on + strand, within RR42_RS18730 at 4057.911 kb on - strand, within RR42_RS18730 at 4057.911 kb on - strand, within RR42_RS18730 at 4058.001 kb on + strand at 4058.001 kb on + strand at 4058.541 kb on - strand at 4058.543 kb on + strand at 4058.544 kb on - strand at 4058.544 kb on - strand at 4058.684 kb on + strand at 4058.685 kb on - strand at 4058.900 kb on - strand at 4058.939 kb on + strand at 4058.939 kb on + strand at 4058.939 kb on + strand at 4058.940 kb on - strand at 4058.945 kb on + strand at 4058.946 kb on - strand at 4058.946 kb on - strand at 4058.986 kb on + strand, within RR42_RS18740 at 4058.987 kb on - strand, within RR42_RS18740 at 4058.987 kb on - strand, within RR42_RS18740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 4,057,163 - -2.2 4,057,253 + +1.4 4,057,254 - +0.6 4,057,254 - +0.3 4,057,254 - +0.5 4,057,306 + +0.9 4,057,306 + +0.2 4,057,306 + +0.3 4,057,307 - +0.1 4,057,307 - -0.5 4,057,586 + RR42_RS18725 0.72 +0.5 4,057,586 + RR42_RS18725 0.72 +0.1 4,057,587 - RR42_RS18725 0.72 +1.8 4,057,910 + RR42_RS18730 0.73 -1.9 4,057,911 - RR42_RS18730 0.74 -0.8 4,057,911 - RR42_RS18730 0.74 -0.1 4,058,001 + -0.2 4,058,001 + -0.3 4,058,541 - +0.2 4,058,543 + -0.4 4,058,544 - +0.4 4,058,544 - -0.0 4,058,684 + +0.7 4,058,685 - -0.1 4,058,900 - +0.1 4,058,939 + -0.8 4,058,939 + -1.3 4,058,939 + -0.5 4,058,940 - -0.7 4,058,945 + +1.7 4,058,946 - +0.2 4,058,946 - +0.1 4,058,986 + RR42_RS18740 0.10 +0.5 4,058,987 - RR42_RS18740 0.11 +1.5 4,058,987 - RR42_RS18740 0.11 +0.6
Or see this region's nucleotide sequence