Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18445 and RR42_RS18450 overlap by 4 nucleotides RR42_RS18450 and RR42_RS18455 are separated by 157 nucleotides RR42_RS18455 and RR42_RS18460 are separated by 68 nucleotides
RR42_RS18445: RR42_RS18445 - hypothetical protein, at 3,993,929 to 3,995,050
_RS18445
RR42_RS18450: RR42_RS18450 - hypothetical protein, at 3,995,047 to 3,996,102
_RS18450
RR42_RS18455: RR42_RS18455 - S-adenosylhomocysteine nucleosidase, at 3,996,260 to 3,997,027
_RS18455
RR42_RS18460: RR42_RS18460 - membrane protein, at 3,997,096 to 3,997,332
_RS18460
Position (kb)
3995
3996
3997 Strain fitness (log2 ratio)
-1
0
1
2 at 3994.048 kb on - strand, within RR42_RS18445 at 3994.054 kb on + strand, within RR42_RS18445 at 3994.055 kb on - strand, within RR42_RS18445 at 3994.220 kb on + strand, within RR42_RS18445 at 3994.220 kb on + strand, within RR42_RS18445 at 3994.220 kb on + strand, within RR42_RS18445 at 3994.221 kb on - strand, within RR42_RS18445 at 3994.221 kb on - strand, within RR42_RS18445 at 3994.221 kb on - strand, within RR42_RS18445 at 3994.221 kb on - strand, within RR42_RS18445 at 3994.293 kb on + strand, within RR42_RS18445 at 3994.293 kb on + strand, within RR42_RS18445 at 3994.293 kb on + strand, within RR42_RS18445 at 3994.293 kb on + strand, within RR42_RS18445 at 3994.294 kb on - strand, within RR42_RS18445 at 3994.294 kb on - strand, within RR42_RS18445 at 3994.583 kb on + strand, within RR42_RS18445 at 3994.632 kb on + strand, within RR42_RS18445 at 3994.632 kb on + strand, within RR42_RS18445 at 3994.632 kb on + strand, within RR42_RS18445 at 3994.632 kb on + strand, within RR42_RS18445 at 3994.632 kb on + strand, within RR42_RS18445 at 3994.632 kb on + strand, within RR42_RS18445 at 3994.632 kb on + strand, within RR42_RS18445 at 3994.632 kb on + strand, within RR42_RS18445 at 3994.633 kb on - strand, within RR42_RS18445 at 3994.633 kb on - strand, within RR42_RS18445 at 3994.633 kb on - strand, within RR42_RS18445 at 3994.633 kb on - strand, within RR42_RS18445 at 3994.633 kb on - strand, within RR42_RS18445 at 3994.719 kb on + strand, within RR42_RS18445 at 3995.027 kb on + strand at 3995.028 kb on - strand at 3995.028 kb on - strand at 3995.028 kb on - strand at 3995.030 kb on + strand at 3995.030 kb on + strand at 3995.030 kb on + strand at 3995.031 kb on - strand at 3995.031 kb on - strand at 3995.031 kb on - strand at 3995.031 kb on - strand at 3995.294 kb on - strand, within RR42_RS18450 at 3995.384 kb on + strand, within RR42_RS18450 at 3995.384 kb on + strand, within RR42_RS18450 at 3995.385 kb on - strand, within RR42_RS18450 at 3995.385 kb on - strand, within RR42_RS18450 at 3995.590 kb on + strand, within RR42_RS18450 at 3995.590 kb on + strand, within RR42_RS18450 at 3995.714 kb on + strand, within RR42_RS18450 at 3995.714 kb on + strand, within RR42_RS18450 at 3995.715 kb on - strand, within RR42_RS18450 at 3995.715 kb on - strand, within RR42_RS18450 at 3995.715 kb on - strand, within RR42_RS18450 at 3995.715 kb on - strand, within RR42_RS18450 at 3996.122 kb on + strand at 3996.122 kb on + strand at 3996.185 kb on + strand at 3996.223 kb on + strand at 3996.223 kb on + strand at 3996.272 kb on - strand at 3996.369 kb on - strand, within RR42_RS18455 at 3996.369 kb on - strand, within RR42_RS18455 at 3996.369 kb on - strand, within RR42_RS18455 at 3996.386 kb on + strand, within RR42_RS18455 at 3996.646 kb on + strand, within RR42_RS18455 at 3996.646 kb on + strand, within RR42_RS18455 at 3996.646 kb on + strand, within RR42_RS18455 at 3996.678 kb on - strand, within RR42_RS18455 at 3996.884 kb on + strand, within RR42_RS18455 at 3996.885 kb on - strand, within RR42_RS18455 at 3996.885 kb on - strand, within RR42_RS18455 at 3996.983 kb on + strand at 3996.984 kb on - strand at 3997.055 kb on + strand at 3997.056 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,994,048 - RR42_RS18445 0.11 -0.3 3,994,054 + RR42_RS18445 0.11 -0.3 3,994,055 - RR42_RS18445 0.11 +0.4 3,994,220 + RR42_RS18445 0.26 -0.1 3,994,220 + RR42_RS18445 0.26 +0.4 3,994,220 + RR42_RS18445 0.26 -1.5 3,994,221 - RR42_RS18445 0.26 +0.6 3,994,221 - RR42_RS18445 0.26 +0.2 3,994,221 - RR42_RS18445 0.26 +0.5 3,994,221 - RR42_RS18445 0.26 +0.2 3,994,293 + RR42_RS18445 0.32 +0.8 3,994,293 + RR42_RS18445 0.32 -0.1 3,994,293 + RR42_RS18445 0.32 -1.3 3,994,293 + RR42_RS18445 0.32 +0.5 3,994,294 - RR42_RS18445 0.33 +0.6 3,994,294 - RR42_RS18445 0.33 +0.8 3,994,583 + RR42_RS18445 0.58 +0.1 3,994,632 + RR42_RS18445 0.63 -0.2 3,994,632 + RR42_RS18445 0.63 -0.1 3,994,632 + RR42_RS18445 0.63 -1.1 3,994,632 + RR42_RS18445 0.63 +0.2 3,994,632 + RR42_RS18445 0.63 +0.9 3,994,632 + RR42_RS18445 0.63 -0.1 3,994,632 + RR42_RS18445 0.63 +0.4 3,994,632 + RR42_RS18445 0.63 +0.6 3,994,633 - RR42_RS18445 0.63 -0.6 3,994,633 - RR42_RS18445 0.63 -0.4 3,994,633 - RR42_RS18445 0.63 +0.8 3,994,633 - RR42_RS18445 0.63 -0.5 3,994,633 - RR42_RS18445 0.63 -0.9 3,994,719 + RR42_RS18445 0.70 +0.4 3,995,027 + -0.3 3,995,028 - -0.1 3,995,028 - +0.5 3,995,028 - -0.7 3,995,030 + +0.1 3,995,030 + -0.1 3,995,030 + +0.1 3,995,031 - -0.5 3,995,031 - -1.2 3,995,031 - -0.1 3,995,031 - +0.9 3,995,294 - RR42_RS18450 0.23 +0.3 3,995,384 + RR42_RS18450 0.32 -1.0 3,995,384 + RR42_RS18450 0.32 -0.7 3,995,385 - RR42_RS18450 0.32 +0.0 3,995,385 - RR42_RS18450 0.32 -0.3 3,995,590 + RR42_RS18450 0.51 -0.0 3,995,590 + RR42_RS18450 0.51 -0.3 3,995,714 + RR42_RS18450 0.63 -0.1 3,995,714 + RR42_RS18450 0.63 -0.8 3,995,715 - RR42_RS18450 0.63 -0.8 3,995,715 - RR42_RS18450 0.63 -0.5 3,995,715 - RR42_RS18450 0.63 +0.1 3,995,715 - RR42_RS18450 0.63 +0.5 3,996,122 + -0.3 3,996,122 + -0.2 3,996,185 + -0.3 3,996,223 + +0.0 3,996,223 + +0.8 3,996,272 - +2.0 3,996,369 - RR42_RS18455 0.14 -0.9 3,996,369 - RR42_RS18455 0.14 -0.3 3,996,369 - RR42_RS18455 0.14 +0.8 3,996,386 + RR42_RS18455 0.16 +1.4 3,996,646 + RR42_RS18455 0.50 +0.2 3,996,646 + RR42_RS18455 0.50 +0.4 3,996,646 + RR42_RS18455 0.50 +0.0 3,996,678 - RR42_RS18455 0.54 +0.1 3,996,884 + RR42_RS18455 0.81 +1.9 3,996,885 - RR42_RS18455 0.81 +0.1 3,996,885 - RR42_RS18455 0.81 +0.3 3,996,983 + +0.8 3,996,984 - -0.0 3,997,055 + +0.6 3,997,056 - -0.6
Or see this region's nucleotide sequence