Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17835 and RR42_RS17840 are separated by 184 nucleotides RR42_RS17840 and RR42_RS17845 are separated by 124 nucleotides
RR42_RS17835: RR42_RS17835 - cytochrome P450, at 3,856,817 to 3,859,165
_RS17835
RR42_RS17840: RR42_RS17840 - transcriptional regulator, at 3,859,350 to 3,860,270
_RS17840
RR42_RS17845: RR42_RS17845 - multidrug DMT transporter permease, at 3,860,395 to 3,861,318
_RS17845
Position (kb)
3859
3860
3861 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3858.369 kb on - strand, within RR42_RS17835 at 3858.369 kb on - strand, within RR42_RS17835 at 3858.617 kb on + strand, within RR42_RS17835 at 3858.618 kb on - strand, within RR42_RS17835 at 3858.618 kb on - strand, within RR42_RS17835 at 3858.630 kb on - strand, within RR42_RS17835 at 3858.704 kb on + strand, within RR42_RS17835 at 3858.704 kb on + strand, within RR42_RS17835 at 3858.704 kb on + strand, within RR42_RS17835 at 3858.704 kb on + strand, within RR42_RS17835 at 3858.705 kb on - strand, within RR42_RS17835 at 3858.705 kb on - strand, within RR42_RS17835 at 3858.705 kb on - strand, within RR42_RS17835 at 3858.776 kb on + strand, within RR42_RS17835 at 3858.777 kb on - strand, within RR42_RS17835 at 3859.161 kb on + strand at 3859.161 kb on + strand at 3859.162 kb on - strand at 3859.162 kb on - strand at 3859.164 kb on - strand at 3859.381 kb on + strand at 3859.381 kb on + strand at 3859.382 kb on - strand at 3859.429 kb on + strand at 3859.453 kb on + strand, within RR42_RS17840 at 3859.598 kb on + strand, within RR42_RS17840 at 3859.600 kb on + strand, within RR42_RS17840 at 3859.600 kb on + strand, within RR42_RS17840 at 3859.601 kb on - strand, within RR42_RS17840 at 3859.601 kb on - strand, within RR42_RS17840 at 3859.658 kb on + strand, within RR42_RS17840 at 3859.681 kb on + strand, within RR42_RS17840 at 3859.741 kb on + strand, within RR42_RS17840 at 3859.741 kb on + strand, within RR42_RS17840 at 3859.742 kb on - strand, within RR42_RS17840 at 3859.742 kb on - strand, within RR42_RS17840 at 3859.900 kb on + strand, within RR42_RS17840 at 3860.050 kb on + strand, within RR42_RS17840 at 3860.050 kb on + strand, within RR42_RS17840 at 3860.050 kb on + strand, within RR42_RS17840 at 3860.056 kb on + strand, within RR42_RS17840 at 3860.056 kb on + strand, within RR42_RS17840 at 3860.057 kb on - strand, within RR42_RS17840 at 3860.057 kb on - strand, within RR42_RS17840 at 3860.057 kb on - strand, within RR42_RS17840 at 3860.300 kb on + strand at 3860.301 kb on - strand at 3860.307 kb on + strand at 3860.308 kb on - strand at 3860.417 kb on + strand at 3860.417 kb on + strand at 3860.418 kb on - strand at 3860.418 kb on - strand at 3860.471 kb on - strand at 3860.471 kb on - strand at 3860.471 kb on - strand at 3860.525 kb on + strand, within RR42_RS17845 at 3860.526 kb on - strand, within RR42_RS17845 at 3860.667 kb on - strand, within RR42_RS17845 at 3861.052 kb on + strand, within RR42_RS17845 at 3861.052 kb on + strand, within RR42_RS17845 at 3861.052 kb on + strand, within RR42_RS17845 at 3861.053 kb on - strand, within RR42_RS17845 at 3861.053 kb on - strand, within RR42_RS17845 at 3861.084 kb on - strand, within RR42_RS17845 at 3861.106 kb on + strand, within RR42_RS17845 at 3861.106 kb on + strand, within RR42_RS17845 at 3861.107 kb on - strand, within RR42_RS17845 at 3861.107 kb on - strand, within RR42_RS17845 at 3861.107 kb on - strand, within RR42_RS17845
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,858,369 - RR42_RS17835 0.66 -0.4 3,858,369 - RR42_RS17835 0.66 +1.1 3,858,617 + RR42_RS17835 0.77 -0.3 3,858,618 - RR42_RS17835 0.77 +0.2 3,858,618 - RR42_RS17835 0.77 +1.3 3,858,630 - RR42_RS17835 0.77 +0.6 3,858,704 + RR42_RS17835 0.80 -0.4 3,858,704 + RR42_RS17835 0.80 -0.1 3,858,704 + RR42_RS17835 0.80 +0.7 3,858,704 + RR42_RS17835 0.80 +0.0 3,858,705 - RR42_RS17835 0.80 -0.1 3,858,705 - RR42_RS17835 0.80 -1.1 3,858,705 - RR42_RS17835 0.80 -2.2 3,858,776 + RR42_RS17835 0.83 -0.3 3,858,777 - RR42_RS17835 0.83 -0.3 3,859,161 + +0.6 3,859,161 + +0.1 3,859,162 - -1.1 3,859,162 - -0.4 3,859,164 - -0.9 3,859,381 + -0.3 3,859,381 + +0.1 3,859,382 - +0.0 3,859,429 + +0.4 3,859,453 + RR42_RS17840 0.11 +0.2 3,859,598 + RR42_RS17840 0.27 -0.1 3,859,600 + RR42_RS17840 0.27 -0.7 3,859,600 + RR42_RS17840 0.27 -0.0 3,859,601 - RR42_RS17840 0.27 -0.3 3,859,601 - RR42_RS17840 0.27 -0.1 3,859,658 + RR42_RS17840 0.33 +0.3 3,859,681 + RR42_RS17840 0.36 -0.7 3,859,741 + RR42_RS17840 0.42 +1.3 3,859,741 + RR42_RS17840 0.42 +0.2 3,859,742 - RR42_RS17840 0.43 +0.1 3,859,742 - RR42_RS17840 0.43 -1.1 3,859,900 + RR42_RS17840 0.60 -0.1 3,860,050 + RR42_RS17840 0.76 -0.5 3,860,050 + RR42_RS17840 0.76 +0.2 3,860,050 + RR42_RS17840 0.76 +0.2 3,860,056 + RR42_RS17840 0.77 -0.2 3,860,056 + RR42_RS17840 0.77 -1.0 3,860,057 - RR42_RS17840 0.77 -1.7 3,860,057 - RR42_RS17840 0.77 -0.2 3,860,057 - RR42_RS17840 0.77 -0.2 3,860,300 + +0.1 3,860,301 - -0.2 3,860,307 + -0.8 3,860,308 - -1.3 3,860,417 + -0.9 3,860,417 + +0.4 3,860,418 - +0.0 3,860,418 - -0.2 3,860,471 - -1.2 3,860,471 - -0.7 3,860,471 - -0.6 3,860,525 + RR42_RS17845 0.14 -0.9 3,860,526 - RR42_RS17845 0.14 +1.4 3,860,667 - RR42_RS17845 0.29 +0.6 3,861,052 + RR42_RS17845 0.71 +2.1 3,861,052 + RR42_RS17845 0.71 -1.5 3,861,052 + RR42_RS17845 0.71 +0.1 3,861,053 - RR42_RS17845 0.71 +0.6 3,861,053 - RR42_RS17845 0.71 -0.4 3,861,084 - RR42_RS17845 0.75 -0.5 3,861,106 + RR42_RS17845 0.77 +1.5 3,861,106 + RR42_RS17845 0.77 -0.7 3,861,107 - RR42_RS17845 0.77 +0.9 3,861,107 - RR42_RS17845 0.77 -0.2 3,861,107 - RR42_RS17845 0.77 -1.1
Or see this region's nucleotide sequence