Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17345 and RR42_RS17350 are separated by 34 nucleotides RR42_RS17350 and RR42_RS17355 are separated by 62 nucleotides RR42_RS17355 and RR42_RS17360 are separated by 55 nucleotides
RR42_RS17345: RR42_RS17345 - twitching motility protein PilT, at 3,753,994 to 3,755,133
_RS17345
RR42_RS17350: RR42_RS17350 - twitching motility protein PilT, at 3,755,168 to 3,756,211
_RS17350
RR42_RS17355: RR42_RS17355 - hypothetical protein, at 3,756,274 to 3,757,002
_RS17355
RR42_RS17360: RR42_RS17360 - pyrroline-5-carboxylate reductase, at 3,757,058 to 3,757,882
_RS17360
Position (kb)
3755
3756
3757 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3754.461 kb on - strand, within RR42_RS17345 at 3754.472 kb on + strand, within RR42_RS17345 at 3754.619 kb on + strand, within RR42_RS17345 at 3754.656 kb on - strand, within RR42_RS17345 at 3755.105 kb on + strand at 3755.105 kb on + strand at 3755.106 kb on - strand at 3755.106 kb on - strand at 3755.106 kb on - strand at 3755.218 kb on - strand at 3755.304 kb on + strand, within RR42_RS17350 at 3755.304 kb on + strand, within RR42_RS17350 at 3755.305 kb on - strand, within RR42_RS17350 at 3755.305 kb on - strand, within RR42_RS17350 at 3755.903 kb on + strand, within RR42_RS17350 at 3755.904 kb on - strand, within RR42_RS17350 at 3755.904 kb on - strand, within RR42_RS17350 at 3755.904 kb on - strand, within RR42_RS17350 at 3755.904 kb on - strand, within RR42_RS17350 at 3755.955 kb on + strand, within RR42_RS17350 at 3755.955 kb on + strand, within RR42_RS17350 at 3755.956 kb on - strand, within RR42_RS17350 at 3755.956 kb on - strand, within RR42_RS17350 at 3756.024 kb on + strand, within RR42_RS17350 at 3756.024 kb on + strand, within RR42_RS17350 at 3756.057 kb on + strand, within RR42_RS17350 at 3756.057 kb on + strand, within RR42_RS17350 at 3756.057 kb on + strand, within RR42_RS17350 at 3756.057 kb on + strand, within RR42_RS17350 at 3756.058 kb on - strand, within RR42_RS17350 at 3756.058 kb on - strand, within RR42_RS17350 at 3756.058 kb on - strand, within RR42_RS17350 at 3756.058 kb on - strand, within RR42_RS17350 at 3756.058 kb on - strand, within RR42_RS17350 at 3756.113 kb on - strand at 3756.191 kb on + strand at 3756.191 kb on + strand at 3756.195 kb on - strand at 3756.213 kb on + strand at 3756.213 kb on + strand at 3756.213 kb on + strand at 3756.213 kb on + strand at 3756.214 kb on - strand at 3756.214 kb on - strand at 3756.214 kb on - strand at 3756.214 kb on - strand at 3756.215 kb on + strand at 3756.215 kb on + strand at 3756.238 kb on + strand at 3756.238 kb on + strand at 3756.249 kb on + strand at 3756.259 kb on + strand at 3756.273 kb on + strand at 3756.445 kb on + strand, within RR42_RS17355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,754,461 - RR42_RS17345 0.41 -1.2 3,754,472 + RR42_RS17345 0.42 +0.3 3,754,619 + RR42_RS17345 0.55 +0.4 3,754,656 - RR42_RS17345 0.58 +0.1 3,755,105 + -0.2 3,755,105 + -0.3 3,755,106 - +0.7 3,755,106 - -0.0 3,755,106 - +0.2 3,755,218 - -1.4 3,755,304 + RR42_RS17350 0.13 +0.3 3,755,304 + RR42_RS17350 0.13 -0.9 3,755,305 - RR42_RS17350 0.13 +0.0 3,755,305 - RR42_RS17350 0.13 -0.1 3,755,903 + RR42_RS17350 0.70 -0.3 3,755,904 - RR42_RS17350 0.70 -0.1 3,755,904 - RR42_RS17350 0.70 +0.4 3,755,904 - RR42_RS17350 0.70 -0.7 3,755,904 - RR42_RS17350 0.70 +2.8 3,755,955 + RR42_RS17350 0.75 +0.3 3,755,955 + RR42_RS17350 0.75 -1.0 3,755,956 - RR42_RS17350 0.75 +0.1 3,755,956 - RR42_RS17350 0.75 -0.2 3,756,024 + RR42_RS17350 0.82 -0.1 3,756,024 + RR42_RS17350 0.82 +0.6 3,756,057 + RR42_RS17350 0.85 -0.1 3,756,057 + RR42_RS17350 0.85 +0.3 3,756,057 + RR42_RS17350 0.85 +0.7 3,756,057 + RR42_RS17350 0.85 -1.0 3,756,058 - RR42_RS17350 0.85 -0.2 3,756,058 - RR42_RS17350 0.85 +0.3 3,756,058 - RR42_RS17350 0.85 +0.7 3,756,058 - RR42_RS17350 0.85 -0.2 3,756,058 - RR42_RS17350 0.85 -0.6 3,756,113 - -1.6 3,756,191 + -0.3 3,756,191 + -0.1 3,756,195 - -0.0 3,756,213 + +0.4 3,756,213 + -1.3 3,756,213 + -0.2 3,756,213 + -0.0 3,756,214 - -0.1 3,756,214 - -0.3 3,756,214 - -1.1 3,756,214 - -0.7 3,756,215 + +0.3 3,756,215 + +0.2 3,756,238 + +1.1 3,756,238 + -0.6 3,756,249 + +0.5 3,756,259 + -0.3 3,756,273 + -0.3 3,756,445 + RR42_RS17355 0.23 -2.6
Or see this region's nucleotide sequence