Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16985 and RR42_RS16990 are separated by 50 nucleotides RR42_RS16990 and RR42_RS16995 are separated by 90 nucleotides RR42_RS16995 and RR42_RS17000 are separated by 200 nucleotides RR42_RS17000 and RR42_RS17005 are separated by 13 nucleotides RR42_RS17005 and RR42_RS17010 are separated by 218 nucleotides
RR42_RS16985: RR42_RS16985 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 3,676,992 to 3,677,954
_RS16985
RR42_RS16990: RR42_RS16990 - peptidylprolyl isomerase, at 3,678,005 to 3,678,502
_RS16990
RR42_RS16995: RR42_RS16995 - hypothetical protein, at 3,678,593 to 3,679,267
_RS16995
RR42_RS17000: RR42_RS17000 - 50S ribosomal protein L28, at 3,679,468 to 3,679,701
_RS17000
RR42_RS17005: RR42_RS17005 - 50S ribosomal protein L33, at 3,679,715 to 3,679,885
_RS17005
RR42_RS17010: RR42_RS17010 - L-aspartate oxidase, at 3,680,104 to 3,681,702
_RS17010
Position (kb)
3678
3679
3680 Strain fitness (log2 ratio)
-2
-1
0
1 at 3678.115 kb on - strand, within RR42_RS16990 at 3678.503 kb on - strand at 3678.503 kb on - strand at 3678.503 kb on - strand at 3678.522 kb on - strand at 3678.529 kb on - strand at 3678.533 kb on + strand at 3678.534 kb on - strand at 3678.534 kb on - strand at 3678.534 kb on - strand at 3678.601 kb on + strand at 3678.827 kb on + strand, within RR42_RS16995 at 3678.827 kb on + strand, within RR42_RS16995 at 3678.827 kb on + strand, within RR42_RS16995 at 3678.827 kb on + strand, within RR42_RS16995 at 3678.827 kb on + strand, within RR42_RS16995 at 3678.827 kb on + strand, within RR42_RS16995 at 3678.827 kb on + strand, within RR42_RS16995 at 3678.828 kb on - strand, within RR42_RS16995 at 3678.828 kb on - strand, within RR42_RS16995 at 3678.828 kb on - strand, within RR42_RS16995 at 3678.828 kb on - strand, within RR42_RS16995 at 3678.828 kb on - strand, within RR42_RS16995 at 3678.926 kb on + strand, within RR42_RS16995 at 3678.927 kb on - strand, within RR42_RS16995 at 3678.956 kb on + strand, within RR42_RS16995 at 3678.956 kb on + strand, within RR42_RS16995 at 3678.956 kb on + strand, within RR42_RS16995 at 3678.956 kb on + strand, within RR42_RS16995 at 3678.956 kb on + strand, within RR42_RS16995 at 3678.957 kb on - strand, within RR42_RS16995 at 3678.957 kb on - strand, within RR42_RS16995 at 3678.957 kb on - strand, within RR42_RS16995 at 3678.957 kb on - strand, within RR42_RS16995 at 3679.082 kb on + strand, within RR42_RS16995 at 3679.082 kb on + strand, within RR42_RS16995 at 3679.082 kb on + strand, within RR42_RS16995 at 3679.082 kb on + strand, within RR42_RS16995 at 3679.083 kb on - strand, within RR42_RS16995 at 3679.083 kb on - strand, within RR42_RS16995 at 3679.229 kb on - strand at 3679.231 kb on + strand at 3679.232 kb on - strand at 3679.883 kb on + strand at 3679.883 kb on + strand at 3679.884 kb on - strand at 3679.969 kb on + strand at 3679.969 kb on + strand at 3679.969 kb on + strand at 3680.049 kb on + strand at 3680.050 kb on - strand at 3680.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,678,115 - RR42_RS16990 0.22 -0.2 3,678,503 - -1.2 3,678,503 - -0.5 3,678,503 - -0.7 3,678,522 - -0.1 3,678,529 - +1.3 3,678,533 + +0.0 3,678,534 - -0.5 3,678,534 - -0.1 3,678,534 - +0.6 3,678,601 + -0.8 3,678,827 + RR42_RS16995 0.35 -1.4 3,678,827 + RR42_RS16995 0.35 -0.6 3,678,827 + RR42_RS16995 0.35 +0.1 3,678,827 + RR42_RS16995 0.35 +0.0 3,678,827 + RR42_RS16995 0.35 -0.3 3,678,827 + RR42_RS16995 0.35 +0.4 3,678,827 + RR42_RS16995 0.35 -0.2 3,678,828 - RR42_RS16995 0.35 +0.4 3,678,828 - RR42_RS16995 0.35 -0.4 3,678,828 - RR42_RS16995 0.35 -0.3 3,678,828 - RR42_RS16995 0.35 +0.5 3,678,828 - RR42_RS16995 0.35 -0.5 3,678,926 + RR42_RS16995 0.49 -0.5 3,678,927 - RR42_RS16995 0.49 -1.2 3,678,956 + RR42_RS16995 0.54 -0.8 3,678,956 + RR42_RS16995 0.54 +0.3 3,678,956 + RR42_RS16995 0.54 +0.2 3,678,956 + RR42_RS16995 0.54 -0.5 3,678,956 + RR42_RS16995 0.54 -0.1 3,678,957 - RR42_RS16995 0.54 +0.8 3,678,957 - RR42_RS16995 0.54 +0.2 3,678,957 - RR42_RS16995 0.54 -1.0 3,678,957 - RR42_RS16995 0.54 -0.7 3,679,082 + RR42_RS16995 0.72 -0.3 3,679,082 + RR42_RS16995 0.72 -0.1 3,679,082 + RR42_RS16995 0.72 -0.5 3,679,082 + RR42_RS16995 0.72 +0.1 3,679,083 - RR42_RS16995 0.73 -0.1 3,679,083 - RR42_RS16995 0.73 -1.2 3,679,229 - -0.5 3,679,231 + -0.7 3,679,232 - +0.1 3,679,883 + -0.5 3,679,883 + -2.1 3,679,884 - -0.1 3,679,969 + -0.3 3,679,969 + -0.0 3,679,969 + +0.0 3,680,049 + -0.1 3,680,050 - -1.4 3,680,242 - +0.8
Or see this region's nucleotide sequence