Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16860 and RR42_RS16865 are separated by 3 nucleotides RR42_RS16865 and RR42_RS16870 overlap by 4 nucleotides RR42_RS16870 and RR42_RS16875 are separated by 17 nucleotides
RR42_RS16860: RR42_RS16860 - pilus assembly protein PilE, at 3,647,752 to 3,648,180
_RS16860
RR42_RS16865: RR42_RS16865 - general secretion pathway protein GspH, at 3,648,184 to 3,648,672
_RS16865
RR42_RS16870: RR42_RS16870 - pilus assembly protein, at 3,648,669 to 3,649,259
_RS16870
RR42_RS16875: RR42_RS16875 - type 4 fimbrial biogenesis PilW transmembrane, at 3,649,277 to 3,650,290
_RS16875
Position (kb)
3648
3649 Strain fitness (log2 ratio)
-1
0
1 at 3647.239 kb on + strand at 3647.258 kb on - strand at 3647.354 kb on + strand at 3647.552 kb on + strand at 3647.552 kb on + strand at 3647.608 kb on - strand at 3647.652 kb on - strand at 3647.721 kb on - strand at 3647.721 kb on - strand at 3647.790 kb on + strand at 3647.866 kb on + strand, within RR42_RS16860 at 3647.867 kb on - strand, within RR42_RS16860 at 3647.867 kb on - strand, within RR42_RS16860 at 3647.867 kb on - strand, within RR42_RS16860 at 3647.867 kb on - strand, within RR42_RS16860 at 3647.935 kb on + strand, within RR42_RS16860 at 3647.936 kb on - strand, within RR42_RS16860 at 3647.983 kb on + strand, within RR42_RS16860 at 3648.017 kb on - strand, within RR42_RS16860 at 3648.058 kb on + strand, within RR42_RS16860 at 3648.059 kb on - strand, within RR42_RS16860 at 3648.059 kb on - strand, within RR42_RS16860 at 3648.120 kb on + strand, within RR42_RS16860 at 3648.120 kb on + strand, within RR42_RS16860 at 3648.120 kb on + strand, within RR42_RS16860 at 3648.178 kb on + strand at 3648.179 kb on - strand at 3648.269 kb on + strand, within RR42_RS16865 at 3648.337 kb on - strand, within RR42_RS16865 at 3648.609 kb on + strand, within RR42_RS16865 at 3648.609 kb on + strand, within RR42_RS16865 at 3648.610 kb on - strand, within RR42_RS16865 at 3648.610 kb on - strand, within RR42_RS16865 at 3648.828 kb on + strand, within RR42_RS16870 at 3648.829 kb on - strand, within RR42_RS16870 at 3648.857 kb on - strand, within RR42_RS16870 at 3648.857 kb on - strand, within RR42_RS16870 at 3648.906 kb on + strand, within RR42_RS16870 at 3648.907 kb on - strand, within RR42_RS16870 at 3648.907 kb on - strand, within RR42_RS16870 at 3648.907 kb on - strand, within RR42_RS16870 at 3649.094 kb on + strand, within RR42_RS16870 at 3649.095 kb on - strand, within RR42_RS16870 at 3649.125 kb on + strand, within RR42_RS16870 at 3649.125 kb on + strand, within RR42_RS16870 at 3649.126 kb on - strand, within RR42_RS16870 at 3649.225 kb on + strand at 3649.238 kb on - strand at 3649.238 kb on - strand at 3649.367 kb on + strand at 3649.434 kb on - strand, within RR42_RS16875 at 3649.498 kb on + strand, within RR42_RS16875 at 3649.498 kb on + strand, within RR42_RS16875 at 3649.498 kb on + strand, within RR42_RS16875 at 3649.498 kb on + strand, within RR42_RS16875
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,647,239 + -0.1 3,647,258 - -0.5 3,647,354 + -0.7 3,647,552 + +0.4 3,647,552 + +0.4 3,647,608 - +0.3 3,647,652 - +0.6 3,647,721 - -0.5 3,647,721 - -0.1 3,647,790 + +0.2 3,647,866 + RR42_RS16860 0.27 +0.2 3,647,867 - RR42_RS16860 0.27 +1.2 3,647,867 - RR42_RS16860 0.27 +0.3 3,647,867 - RR42_RS16860 0.27 -0.1 3,647,867 - RR42_RS16860 0.27 +0.4 3,647,935 + RR42_RS16860 0.43 +0.4 3,647,936 - RR42_RS16860 0.43 -0.6 3,647,983 + RR42_RS16860 0.54 -1.0 3,648,017 - RR42_RS16860 0.62 +0.4 3,648,058 + RR42_RS16860 0.71 -0.7 3,648,059 - RR42_RS16860 0.72 -0.8 3,648,059 - RR42_RS16860 0.72 -0.2 3,648,120 + RR42_RS16860 0.86 -0.0 3,648,120 + RR42_RS16860 0.86 -1.5 3,648,120 + RR42_RS16860 0.86 -0.6 3,648,178 + -1.7 3,648,179 - -0.1 3,648,269 + RR42_RS16865 0.17 -0.1 3,648,337 - RR42_RS16865 0.31 -0.2 3,648,609 + RR42_RS16865 0.87 +0.1 3,648,609 + RR42_RS16865 0.87 -0.4 3,648,610 - RR42_RS16865 0.87 -0.1 3,648,610 - RR42_RS16865 0.87 -0.0 3,648,828 + RR42_RS16870 0.27 +0.3 3,648,829 - RR42_RS16870 0.27 +0.2 3,648,857 - RR42_RS16870 0.32 +0.0 3,648,857 - RR42_RS16870 0.32 +1.1 3,648,906 + RR42_RS16870 0.40 -1.0 3,648,907 - RR42_RS16870 0.40 +0.3 3,648,907 - RR42_RS16870 0.40 -0.2 3,648,907 - RR42_RS16870 0.40 +0.2 3,649,094 + RR42_RS16870 0.72 -0.3 3,649,095 - RR42_RS16870 0.72 -0.5 3,649,125 + RR42_RS16870 0.77 -0.4 3,649,125 + RR42_RS16870 0.77 +0.5 3,649,126 - RR42_RS16870 0.77 -0.1 3,649,225 + -0.2 3,649,238 - +0.1 3,649,238 - +0.9 3,649,367 + -1.0 3,649,434 - RR42_RS16875 0.15 -0.4 3,649,498 + RR42_RS16875 0.22 -0.8 3,649,498 + RR42_RS16875 0.22 +0.6 3,649,498 + RR42_RS16875 0.22 -0.7 3,649,498 + RR42_RS16875 0.22 -0.3
Or see this region's nucleotide sequence