Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16775 and RR42_RS16780 are separated by 1 nucleotides RR42_RS16780 and RR42_RS16785 are separated by 308 nucleotides RR42_RS16785 and RR42_RS16790 are separated by 42 nucleotides RR42_RS16790 and RR42_RS16795 are separated by 55 nucleotides
RR42_RS16775: RR42_RS16775 - NADH oxidase, at 3,630,205 to 3,631,341
_RS16775
RR42_RS16780: RR42_RS16780 - hypothetical protein, at 3,631,343 to 3,631,732
_RS16780
RR42_RS16785: RR42_RS16785 - manganese transporter, at 3,632,041 to 3,633,357
_RS16785
RR42_RS16790: RR42_RS16790 - enoyl-CoA hydratase, at 3,633,400 to 3,634,200
_RS16790
RR42_RS16795: RR42_RS16795 - acyl-CoA dehydrogenase, at 3,634,256 to 3,635,407
_RS16795
Position (kb)
3632
3633
3634 Strain fitness (log2 ratio)
-2
-1
0
1 at 3631.051 kb on + strand, within RR42_RS16775 at 3631.226 kb on - strand, within RR42_RS16775 at 3631.279 kb on + strand at 3631.306 kb on + strand at 3631.306 kb on + strand at 3631.306 kb on + strand at 3631.339 kb on + strand at 3631.340 kb on - strand at 3631.502 kb on + strand, within RR42_RS16780 at 3631.503 kb on - strand, within RR42_RS16780 at 3631.576 kb on - strand, within RR42_RS16780 at 3631.576 kb on - strand, within RR42_RS16780 at 3631.576 kb on - strand, within RR42_RS16780 at 3631.722 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.763 kb on + strand at 3631.764 kb on - strand at 3631.764 kb on - strand at 3631.766 kb on - strand at 3631.838 kb on + strand at 3631.861 kb on + strand at 3631.862 kb on - strand at 3631.915 kb on + strand at 3631.915 kb on + strand at 3631.916 kb on - strand at 3631.916 kb on - strand at 3631.916 kb on - strand at 3631.916 kb on - strand at 3631.921 kb on + strand at 3631.923 kb on + strand at 3631.923 kb on + strand at 3631.923 kb on + strand at 3631.924 kb on - strand at 3631.924 kb on - strand at 3631.924 kb on - strand at 3631.966 kb on - strand at 3632.020 kb on + strand at 3632.020 kb on + strand at 3632.020 kb on + strand at 3632.020 kb on + strand at 3632.021 kb on - strand at 3632.021 kb on - strand at 3632.149 kb on + strand at 3632.149 kb on + strand at 3632.150 kb on - strand at 3632.150 kb on - strand at 3632.204 kb on - strand, within RR42_RS16785 at 3632.701 kb on + strand, within RR42_RS16785 at 3632.702 kb on - strand, within RR42_RS16785 at 3632.728 kb on + strand, within RR42_RS16785 at 3632.906 kb on + strand, within RR42_RS16785 at 3632.906 kb on + strand, within RR42_RS16785 at 3633.182 kb on + strand, within RR42_RS16785 at 3633.183 kb on - strand, within RR42_RS16785 at 3633.385 kb on + strand at 3633.385 kb on + strand at 3633.385 kb on + strand at 3633.386 kb on - strand at 3633.386 kb on - strand at 3633.394 kb on - strand at 3633.755 kb on + strand, within RR42_RS16790 at 3633.755 kb on + strand, within RR42_RS16790 at 3633.756 kb on - strand, within RR42_RS16790 at 3633.895 kb on + strand, within RR42_RS16790 at 3633.923 kb on + strand, within RR42_RS16790 at 3634.002 kb on + strand, within RR42_RS16790 at 3634.002 kb on + strand, within RR42_RS16790 at 3634.005 kb on + strand, within RR42_RS16790 at 3634.114 kb on - strand, within RR42_RS16790 at 3634.212 kb on + strand at 3634.311 kb on + strand at 3634.322 kb on + strand at 3634.323 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,631,051 + RR42_RS16775 0.74 +0.6 3,631,226 - RR42_RS16775 0.90 -0.4 3,631,279 + +0.3 3,631,306 + -0.4 3,631,306 + +0.5 3,631,306 + +0.6 3,631,339 + +1.2 3,631,340 - -2.3 3,631,502 + RR42_RS16780 0.41 +0.4 3,631,503 - RR42_RS16780 0.41 +0.9 3,631,576 - RR42_RS16780 0.60 -0.1 3,631,576 - RR42_RS16780 0.60 -0.9 3,631,576 - RR42_RS16780 0.60 +0.3 3,631,722 + -0.2 3,631,763 + +1.0 3,631,763 + -0.2 3,631,763 + -0.4 3,631,763 + +0.4 3,631,763 + +0.3 3,631,763 + -0.6 3,631,763 + +0.0 3,631,763 + -0.6 3,631,763 + +0.1 3,631,763 + +0.2 3,631,763 + +0.1 3,631,763 + +0.5 3,631,764 - +0.6 3,631,764 - -1.1 3,631,766 - -0.0 3,631,838 + -0.3 3,631,861 + +0.7 3,631,862 - -0.1 3,631,915 + -0.9 3,631,915 + -0.3 3,631,916 - -0.9 3,631,916 - -0.4 3,631,916 - -0.4 3,631,916 - -0.2 3,631,921 + +0.0 3,631,923 + -0.2 3,631,923 + -0.2 3,631,923 + +0.7 3,631,924 - +0.4 3,631,924 - +0.8 3,631,924 - +0.3 3,631,966 - -1.2 3,632,020 + +0.7 3,632,020 + -1.1 3,632,020 + +0.2 3,632,020 + -0.8 3,632,021 - -1.6 3,632,021 - +0.1 3,632,149 + +0.2 3,632,149 + -0.4 3,632,150 - +0.3 3,632,150 - -1.2 3,632,204 - RR42_RS16785 0.12 -1.3 3,632,701 + RR42_RS16785 0.50 -0.2 3,632,702 - RR42_RS16785 0.50 -0.3 3,632,728 + RR42_RS16785 0.52 -0.7 3,632,906 + RR42_RS16785 0.66 -1.3 3,632,906 + RR42_RS16785 0.66 -1.7 3,633,182 + RR42_RS16785 0.87 -0.4 3,633,183 - RR42_RS16785 0.87 -0.3 3,633,385 + +0.2 3,633,385 + -0.3 3,633,385 + -0.3 3,633,386 - -0.8 3,633,386 - +0.1 3,633,394 - +0.6 3,633,755 + RR42_RS16790 0.44 +0.8 3,633,755 + RR42_RS16790 0.44 +0.2 3,633,756 - RR42_RS16790 0.44 -0.7 3,633,895 + RR42_RS16790 0.62 +0.2 3,633,923 + RR42_RS16790 0.65 -0.5 3,634,002 + RR42_RS16790 0.75 -0.7 3,634,002 + RR42_RS16790 0.75 -0.3 3,634,005 + RR42_RS16790 0.76 -0.9 3,634,114 - RR42_RS16790 0.89 -0.7 3,634,212 + +0.2 3,634,311 + +0.8 3,634,322 + -0.1 3,634,323 - -1.1
Or see this region's nucleotide sequence