Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15900 and RR42_RS15905 are separated by 79 nucleotides RR42_RS15905 and RR42_RS15910 are separated by 125 nucleotides RR42_RS15910 and RR42_RS15915 are separated by 195 nucleotides
RR42_RS15900: RR42_RS15900 - porphobilinogen deaminase, at 3,437,742 to 3,438,743
_RS15900
RR42_RS15905: RR42_RS15905 - phosphoenolpyruvate carboxylase, at 3,438,823 to 3,441,846
_RS15905
RR42_RS15910: RR42_RS15910 - ferritin, at 3,441,972 to 3,442,607
_RS15910
RR42_RS15915: RR42_RS15915 - RNA polymerase subunit sigma-24, at 3,442,803 to 3,443,408
_RS15915
Position (kb)
3438
3439
3440
3441
3442 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3438.755 kb on - strand at 3438.755 kb on - strand at 3438.798 kb on + strand at 3438.798 kb on + strand at 3438.798 kb on + strand at 3438.798 kb on + strand at 3438.798 kb on + strand at 3438.799 kb on - strand at 3438.799 kb on - strand at 3438.799 kb on - strand at 3438.802 kb on + strand at 3438.802 kb on + strand at 3438.802 kb on + strand at 3438.803 kb on - strand at 3439.258 kb on + strand, within RR42_RS15905 at 3439.259 kb on - strand, within RR42_RS15905 at 3439.660 kb on + strand, within RR42_RS15905 at 3439.661 kb on - strand, within RR42_RS15905 at 3439.663 kb on + strand, within RR42_RS15905 at 3439.663 kb on + strand, within RR42_RS15905 at 3439.956 kb on - strand, within RR42_RS15905 at 3440.053 kb on + strand, within RR42_RS15905 at 3440.053 kb on + strand, within RR42_RS15905 at 3440.053 kb on + strand, within RR42_RS15905 at 3440.053 kb on + strand, within RR42_RS15905 at 3440.054 kb on - strand, within RR42_RS15905 at 3440.054 kb on - strand, within RR42_RS15905 at 3440.054 kb on - strand at 3440.054 kb on - strand, within RR42_RS15905 at 3440.162 kb on + strand, within RR42_RS15905 at 3440.162 kb on + strand, within RR42_RS15905 at 3440.163 kb on - strand, within RR42_RS15905 at 3440.350 kb on + strand, within RR42_RS15905 at 3440.350 kb on + strand, within RR42_RS15905 at 3440.351 kb on - strand, within RR42_RS15905 at 3440.351 kb on - strand, within RR42_RS15905 at 3440.437 kb on + strand, within RR42_RS15905 at 3440.524 kb on + strand, within RR42_RS15905 at 3440.525 kb on - strand, within RR42_RS15905 at 3440.525 kb on - strand, within RR42_RS15905 at 3440.827 kb on + strand, within RR42_RS15905 at 3440.932 kb on + strand, within RR42_RS15905 at 3440.932 kb on + strand, within RR42_RS15905 at 3441.182 kb on - strand, within RR42_RS15905 at 3441.206 kb on - strand, within RR42_RS15905 at 3441.206 kb on - strand, within RR42_RS15905 at 3441.206 kb on - strand, within RR42_RS15905 at 3441.499 kb on - strand, within RR42_RS15905 at 3441.707 kb on - strand at 3442.102 kb on - strand, within RR42_RS15910 at 3442.195 kb on + strand, within RR42_RS15910 at 3442.196 kb on - strand, within RR42_RS15910 at 3442.196 kb on - strand, within RR42_RS15910 at 3442.349 kb on - strand, within RR42_RS15910 at 3442.367 kb on - strand, within RR42_RS15910 at 3442.367 kb on - strand, within RR42_RS15910 at 3442.623 kb on - strand at 3442.705 kb on + strand at 3442.706 kb on - strand at 3442.765 kb on + strand at 3442.765 kb on + strand at 3442.766 kb on - strand at 3442.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,438,755 - -1.1 3,438,755 - -0.7 3,438,798 + -0.6 3,438,798 + -0.7 3,438,798 + -1.2 3,438,798 + +0.2 3,438,798 + -3.5 3,438,799 - +0.3 3,438,799 - -1.2 3,438,799 - -1.4 3,438,802 + -0.9 3,438,802 + +0.4 3,438,802 + -1.0 3,438,803 - -1.1 3,439,258 + RR42_RS15905 0.14 -1.5 3,439,259 - RR42_RS15905 0.14 +0.1 3,439,660 + RR42_RS15905 0.28 +0.8 3,439,661 - RR42_RS15905 0.28 -0.2 3,439,663 + RR42_RS15905 0.28 -0.3 3,439,663 + RR42_RS15905 0.28 -0.8 3,439,956 - RR42_RS15905 0.37 -1.6 3,440,053 + RR42_RS15905 0.41 +0.6 3,440,053 + RR42_RS15905 0.41 +0.3 3,440,053 + RR42_RS15905 0.41 -0.5 3,440,053 + RR42_RS15905 0.41 -0.5 3,440,054 - RR42_RS15905 0.41 +0.1 3,440,054 - RR42_RS15905 0.41 -0.0 3,440,054 - +1.1 3,440,054 - RR42_RS15905 0.41 -2.7 3,440,162 + RR42_RS15905 0.44 -0.4 3,440,162 + RR42_RS15905 0.44 +1.1 3,440,163 - RR42_RS15905 0.44 -0.7 3,440,350 + RR42_RS15905 0.50 -0.7 3,440,350 + RR42_RS15905 0.50 -0.6 3,440,351 - RR42_RS15905 0.51 -0.9 3,440,351 - RR42_RS15905 0.51 -0.5 3,440,437 + RR42_RS15905 0.53 -0.5 3,440,524 + RR42_RS15905 0.56 -0.8 3,440,525 - RR42_RS15905 0.56 -2.6 3,440,525 - RR42_RS15905 0.56 -0.7 3,440,827 + RR42_RS15905 0.66 -0.1 3,440,932 + RR42_RS15905 0.70 -1.0 3,440,932 + RR42_RS15905 0.70 -0.1 3,441,182 - RR42_RS15905 0.78 +0.1 3,441,206 - RR42_RS15905 0.79 -2.4 3,441,206 - RR42_RS15905 0.79 -1.4 3,441,206 - RR42_RS15905 0.79 -0.4 3,441,499 - RR42_RS15905 0.88 -0.9 3,441,707 - -1.4 3,442,102 - RR42_RS15910 0.20 -0.0 3,442,195 + RR42_RS15910 0.35 -1.3 3,442,196 - RR42_RS15910 0.35 -0.9 3,442,196 - RR42_RS15910 0.35 +0.1 3,442,349 - RR42_RS15910 0.59 +0.0 3,442,367 - RR42_RS15910 0.62 +0.0 3,442,367 - RR42_RS15910 0.62 +0.6 3,442,623 - -0.2 3,442,705 + -0.7 3,442,706 - -0.5 3,442,765 + -0.3 3,442,765 + -0.8 3,442,766 - -0.0 3,442,766 - -0.1
Or see this region's nucleotide sequence