Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15005 and RR42_RS15010 are separated by 59 nucleotides RR42_RS15010 and RR42_RS15015 overlap by 4 nucleotides RR42_RS15015 and RR42_RS15020 are separated by 358 nucleotides
RR42_RS15005: RR42_RS15005 - 1-deoxy-D-xylulose-5-phosphate synthase, at 3,244,088 to 3,245,998
_RS15005
RR42_RS15010: RR42_RS15010 - geranyl transferase, at 3,246,058 to 3,246,987
_RS15010
RR42_RS15015: RR42_RS15015 - exodeoxyribonuclease VII small subunit, at 3,246,984 to 3,247,274
_RS15015
RR42_RS15020: RR42_RS15020 - (2Fe-2S)-binding protein, at 3,247,633 to 3,248,736
_RS15020
Position (kb)
3246
3247
3248 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3247.109 kb on - strand, within RR42_RS15015 at 3247.263 kb on - strand at 3247.462 kb on + strand at 3247.462 kb on + strand at 3247.463 kb on - strand at 3247.631 kb on + strand at 3247.632 kb on - strand at 3247.697 kb on - strand at 3247.697 kb on - strand at 3247.951 kb on + strand, within RR42_RS15020 at 3247.952 kb on - strand, within RR42_RS15020 at 3248.122 kb on + strand, within RR42_RS15020 at 3248.122 kb on + strand, within RR42_RS15020 at 3248.122 kb on + strand, within RR42_RS15020 at 3248.158 kb on + strand, within RR42_RS15020 at 3248.159 kb on - strand, within RR42_RS15020 at 3248.159 kb on - strand, within RR42_RS15020 at 3248.185 kb on + strand, within RR42_RS15020 at 3248.186 kb on - strand, within RR42_RS15020 at 3248.197 kb on + strand, within RR42_RS15020 at 3248.197 kb on + strand, within RR42_RS15020 at 3248.198 kb on - strand, within RR42_RS15020 at 3248.198 kb on - strand, within RR42_RS15020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,247,109 - RR42_RS15015 0.43 -1.0 3,247,263 - -0.1 3,247,462 + -0.1 3,247,462 + +0.1 3,247,463 - -1.0 3,247,631 + -1.6 3,247,632 - -0.2 3,247,697 - -1.0 3,247,697 - -0.2 3,247,951 + RR42_RS15020 0.29 -0.7 3,247,952 - RR42_RS15020 0.29 -2.3 3,248,122 + RR42_RS15020 0.44 -0.6 3,248,122 + RR42_RS15020 0.44 -0.9 3,248,122 + RR42_RS15020 0.44 -0.8 3,248,158 + RR42_RS15020 0.48 -0.2 3,248,159 - RR42_RS15020 0.48 -0.6 3,248,159 - RR42_RS15020 0.48 -0.9 3,248,185 + RR42_RS15020 0.50 -0.2 3,248,186 - RR42_RS15020 0.50 -0.8 3,248,197 + RR42_RS15020 0.51 +0.8 3,248,197 + RR42_RS15020 0.51 -1.2 3,248,198 - RR42_RS15020 0.51 -2.8 3,248,198 - RR42_RS15020 0.51 -0.2
Or see this region's nucleotide sequence