Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14720 and RR42_RS14725 are separated by 140 nucleotides
RR42_RS14720: RR42_RS14720 - RNA helicase, at 3,171,441 to 3,173,381
_RS14720
RR42_RS14725: RR42_RS14725 - glycosyl transferase, at 3,173,522 to 3,174,781
_RS14725
Position (kb)
3171
3172
3173
3174 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3170.469 kb on + strand at 3170.496 kb on - strand at 3170.519 kb on + strand at 3170.519 kb on + strand at 3170.519 kb on + strand at 3170.519 kb on + strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.655 kb on - strand at 3170.772 kb on - strand at 3170.791 kb on + strand at 3170.791 kb on + strand at 3170.871 kb on + strand at 3170.871 kb on + strand at 3170.872 kb on - strand at 3170.873 kb on + strand at 3170.873 kb on + strand at 3170.873 kb on + strand at 3170.874 kb on - strand at 3170.915 kb on + strand at 3170.915 kb on + strand at 3170.916 kb on - strand at 3170.916 kb on - strand at 3170.916 kb on - strand at 3171.009 kb on + strand at 3171.144 kb on + strand at 3171.144 kb on + strand at 3171.245 kb on + strand at 3171.245 kb on + strand at 3171.245 kb on + strand at 3171.245 kb on + strand at 3171.245 kb on + strand at 3171.247 kb on + strand at 3171.247 kb on + strand at 3171.247 kb on + strand at 3171.247 kb on + strand at 3171.367 kb on + strand at 3171.399 kb on + strand at 3171.960 kb on + strand, within RR42_RS14720 at 3172.659 kb on + strand, within RR42_RS14720 at 3173.432 kb on + strand at 3173.432 kb on + strand at 3173.432 kb on + strand at 3173.433 kb on - strand at 3173.523 kb on + strand at 3173.524 kb on - strand at 3173.664 kb on + strand, within RR42_RS14725 at 3173.664 kb on + strand, within RR42_RS14725 at 3173.664 kb on + strand, within RR42_RS14725 at 3173.664 kb on + strand, within RR42_RS14725 at 3173.665 kb on - strand, within RR42_RS14725 at 3173.665 kb on - strand, within RR42_RS14725 at 3173.665 kb on - strand, within RR42_RS14725 at 3173.709 kb on + strand, within RR42_RS14725 at 3173.710 kb on - strand, within RR42_RS14725 at 3174.009 kb on + strand, within RR42_RS14725 at 3174.009 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.084 kb on + strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.085 kb on - strand, within RR42_RS14725 at 3174.088 kb on + strand, within RR42_RS14725 at 3174.088 kb on + strand, within RR42_RS14725 at 3174.088 kb on + strand, within RR42_RS14725 at 3174.088 kb on + strand, within RR42_RS14725 at 3174.089 kb on - strand, within RR42_RS14725 at 3174.307 kb on - strand, within RR42_RS14725 at 3174.307 kb on - strand, within RR42_RS14725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,170,469 + -1.1 3,170,496 - +0.7 3,170,519 + +0.2 3,170,519 + +0.7 3,170,519 + -0.4 3,170,519 + +0.8 3,170,520 - +1.0 3,170,520 - -0.6 3,170,520 - -0.3 3,170,520 - +0.8 3,170,520 - +0.1 3,170,655 - +0.6 3,170,772 - +0.3 3,170,791 + -1.5 3,170,791 + -0.0 3,170,871 + -0.3 3,170,871 + -0.0 3,170,872 - -1.6 3,170,873 + -0.1 3,170,873 + +0.5 3,170,873 + +0.8 3,170,874 - -2.3 3,170,915 + -3.1 3,170,915 + +0.3 3,170,916 - -0.6 3,170,916 - +0.7 3,170,916 - -0.7 3,171,009 + +0.2 3,171,144 + -0.3 3,171,144 + +0.3 3,171,245 + -0.3 3,171,245 + +0.1 3,171,245 + -1.2 3,171,245 + -0.0 3,171,245 + -1.0 3,171,247 + +0.3 3,171,247 + -0.6 3,171,247 + +0.5 3,171,247 + -0.0 3,171,367 + +0.0 3,171,399 + -0.0 3,171,960 + RR42_RS14720 0.27 -2.4 3,172,659 + RR42_RS14720 0.63 -0.3 3,173,432 + +0.2 3,173,432 + -0.2 3,173,432 + +0.8 3,173,433 - +0.5 3,173,523 + +0.5 3,173,524 - -0.4 3,173,664 + RR42_RS14725 0.11 +1.1 3,173,664 + RR42_RS14725 0.11 +0.4 3,173,664 + RR42_RS14725 0.11 -0.9 3,173,664 + RR42_RS14725 0.11 -0.1 3,173,665 - RR42_RS14725 0.11 +0.4 3,173,665 - RR42_RS14725 0.11 -0.2 3,173,665 - RR42_RS14725 0.11 +0.9 3,173,709 + RR42_RS14725 0.15 -0.6 3,173,710 - RR42_RS14725 0.15 -0.7 3,174,009 + RR42_RS14725 0.39 +0.5 3,174,009 + RR42_RS14725 0.39 +0.1 3,174,084 + RR42_RS14725 0.45 +0.1 3,174,084 + RR42_RS14725 0.45 +0.2 3,174,084 + RR42_RS14725 0.45 +0.3 3,174,084 + RR42_RS14725 0.45 +0.2 3,174,084 + RR42_RS14725 0.45 +1.0 3,174,084 + RR42_RS14725 0.45 -0.3 3,174,084 + RR42_RS14725 0.45 -0.8 3,174,084 + RR42_RS14725 0.45 -0.6 3,174,084 + RR42_RS14725 0.45 -0.8 3,174,084 + RR42_RS14725 0.45 +0.0 3,174,084 + RR42_RS14725 0.45 -0.2 3,174,085 - RR42_RS14725 0.45 -0.1 3,174,085 - RR42_RS14725 0.45 +0.9 3,174,085 - RR42_RS14725 0.45 -0.3 3,174,085 - RR42_RS14725 0.45 -1.3 3,174,085 - RR42_RS14725 0.45 -1.4 3,174,085 - RR42_RS14725 0.45 -0.9 3,174,085 - RR42_RS14725 0.45 +0.1 3,174,085 - RR42_RS14725 0.45 -0.2 3,174,085 - RR42_RS14725 0.45 +0.8 3,174,085 - RR42_RS14725 0.45 -0.0 3,174,088 + RR42_RS14725 0.45 -1.0 3,174,088 + RR42_RS14725 0.45 -0.1 3,174,088 + RR42_RS14725 0.45 +0.7 3,174,088 + RR42_RS14725 0.45 -0.4 3,174,089 - RR42_RS14725 0.45 -0.2 3,174,307 - RR42_RS14725 0.62 -0.5 3,174,307 - RR42_RS14725 0.62 +1.6
Or see this region's nucleotide sequence