Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14525 and RR42_RS14530 are separated by 110 nucleotides RR42_RS14530 and RR42_RS14535 are separated by 457 nucleotides
RR42_RS14525: RR42_RS14525 - cysteine synthase, at 3,126,306 to 3,127,442
_RS14525
RR42_RS14530: RR42_RS14530 - 2-hydroxyacid dehydrogenase, at 3,127,553 to 3,127,948
_RS14530
RR42_RS14535: RR42_RS14535 - hypothetical protein, at 3,128,406 to 3,129,551
_RS14535
Position (kb)
3127
3128 Strain fitness (log2 ratio)
-1
0
1 at 3126.674 kb on + strand, within RR42_RS14525 at 3126.730 kb on - strand, within RR42_RS14525 at 3126.776 kb on - strand, within RR42_RS14525 at 3126.825 kb on - strand, within RR42_RS14525 at 3126.825 kb on - strand, within RR42_RS14525 at 3126.836 kb on - strand, within RR42_RS14525 at 3126.950 kb on + strand, within RR42_RS14525 at 3126.988 kb on + strand, within RR42_RS14525 at 3126.989 kb on - strand, within RR42_RS14525 at 3127.032 kb on - strand, within RR42_RS14525 at 3127.048 kb on + strand, within RR42_RS14525 at 3127.049 kb on - strand, within RR42_RS14525 at 3127.091 kb on - strand, within RR42_RS14525 at 3127.174 kb on + strand, within RR42_RS14525 at 3127.458 kb on - strand at 3127.507 kb on - strand at 3127.644 kb on + strand, within RR42_RS14530 at 3127.644 kb on + strand, within RR42_RS14530 at 3127.645 kb on - strand, within RR42_RS14530 at 3127.765 kb on + strand, within RR42_RS14530 at 3127.766 kb on - strand, within RR42_RS14530 at 3127.858 kb on - strand, within RR42_RS14530 at 3127.858 kb on - strand, within RR42_RS14530 at 3127.977 kb on + strand at 3127.977 kb on + strand at 3128.019 kb on - strand at 3128.055 kb on + strand at 3128.059 kb on - strand at 3128.061 kb on + strand at 3128.062 kb on - strand at 3128.062 kb on - strand at 3128.062 kb on - strand at 3128.192 kb on + strand at 3128.193 kb on - strand at 3128.346 kb on - strand at 3128.424 kb on + strand at 3128.424 kb on + strand at 3128.523 kb on - strand, within RR42_RS14535 at 3128.687 kb on + strand, within RR42_RS14535 at 3128.697 kb on + strand, within RR42_RS14535 at 3128.753 kb on + strand, within RR42_RS14535 at 3128.757 kb on + strand, within RR42_RS14535 at 3128.757 kb on + strand, within RR42_RS14535 at 3128.758 kb on - strand, within RR42_RS14535 at 3128.758 kb on - strand, within RR42_RS14535 at 3128.812 kb on - strand, within RR42_RS14535 at 3128.823 kb on + strand, within RR42_RS14535 at 3128.824 kb on - strand, within RR42_RS14535 at 3128.826 kb on - strand, within RR42_RS14535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,126,674 + RR42_RS14525 0.32 +0.1 3,126,730 - RR42_RS14525 0.37 +0.2 3,126,776 - RR42_RS14525 0.41 -1.2 3,126,825 - RR42_RS14525 0.46 +0.1 3,126,825 - RR42_RS14525 0.46 +1.5 3,126,836 - RR42_RS14525 0.47 -0.7 3,126,950 + RR42_RS14525 0.57 +0.2 3,126,988 + RR42_RS14525 0.60 -0.2 3,126,989 - RR42_RS14525 0.60 +0.6 3,127,032 - RR42_RS14525 0.64 -0.4 3,127,048 + RR42_RS14525 0.65 -0.0 3,127,049 - RR42_RS14525 0.65 +0.6 3,127,091 - RR42_RS14525 0.69 +0.4 3,127,174 + RR42_RS14525 0.76 +0.7 3,127,458 - -0.5 3,127,507 - -0.5 3,127,644 + RR42_RS14530 0.23 -1.1 3,127,644 + RR42_RS14530 0.23 -1.5 3,127,645 - RR42_RS14530 0.23 -0.3 3,127,765 + RR42_RS14530 0.54 -1.1 3,127,766 - RR42_RS14530 0.54 -0.6 3,127,858 - RR42_RS14530 0.77 +0.1 3,127,858 - RR42_RS14530 0.77 -1.1 3,127,977 + +0.2 3,127,977 + +0.7 3,128,019 - -0.2 3,128,055 + -0.3 3,128,059 - -0.4 3,128,061 + -0.9 3,128,062 - -0.6 3,128,062 - +0.4 3,128,062 - +1.4 3,128,192 + +0.2 3,128,193 - -1.2 3,128,346 - -0.0 3,128,424 + +0.4 3,128,424 + +0.4 3,128,523 - RR42_RS14535 0.10 -0.6 3,128,687 + RR42_RS14535 0.25 +0.0 3,128,697 + RR42_RS14535 0.25 -0.1 3,128,753 + RR42_RS14535 0.30 +0.3 3,128,757 + RR42_RS14535 0.31 -0.9 3,128,757 + RR42_RS14535 0.31 +0.6 3,128,758 - RR42_RS14535 0.31 +0.1 3,128,758 - RR42_RS14535 0.31 -0.5 3,128,812 - RR42_RS14535 0.35 -0.3 3,128,823 + RR42_RS14535 0.36 +0.4 3,128,824 - RR42_RS14535 0.36 -0.4 3,128,826 - RR42_RS14535 0.37 -1.3
Or see this region's nucleotide sequence