Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14360 and RR42_RS14365 are separated by 127 nucleotides RR42_RS14365 and RR42_RS14370 overlap by 17 nucleotides RR42_RS14370 and RR42_RS14375 are separated by 187 nucleotides
RR42_RS14360: RR42_RS14360 - tryptophan synthase subunit alpha, at 3,086,924 to 3,088,117
_RS14360
RR42_RS14365: RR42_RS14365 - N-(5'-phosphoribosyl)anthranilate isomerase, at 3,088,245 to 3,088,946
_RS14365
RR42_RS14370: RR42_RS14370 - tRNA pseudouridine synthase A, at 3,088,930 to 3,089,754
_RS14370
RR42_RS14375: RR42_RS14375 - pilus assembly protein FimV, at 3,089,942 to 3,092,824
_RS14375
Position (kb)
3088
3089 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3087.711 kb on + strand, within RR42_RS14360 at 3088.017 kb on + strand at 3088.077 kb on + strand at 3088.335 kb on + strand, within RR42_RS14365 at 3088.335 kb on + strand, within RR42_RS14365 at 3088.710 kb on - strand at 3088.819 kb on + strand, within RR42_RS14365 at 3089.232 kb on - strand at 3089.797 kb on - strand at 3089.929 kb on + strand at 3089.929 kb on + strand at 3089.929 kb on + strand at 3089.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,087,711 + RR42_RS14360 0.66 -0.6 3,088,017 + -2.6 3,088,077 + +0.8 3,088,335 + RR42_RS14365 0.13 -0.4 3,088,335 + RR42_RS14365 0.13 -1.7 3,088,710 - -1.0 3,088,819 + RR42_RS14365 0.82 -2.3 3,089,232 - +2.0 3,089,797 - -0.0 3,089,929 + -0.6 3,089,929 + -0.7 3,089,929 + -0.4 3,089,930 - +0.7
Or see this region's nucleotide sequence