Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12385 and RR42_RS12390 are separated by 164 nucleotides RR42_RS12390 and RR42_RS12395 are separated by 231 nucleotides
RR42_RS12385: RR42_RS12385 - formamidase, at 2,656,515 to 2,657,747
_RS12385
RR42_RS12390: RR42_RS12390 - transporter, at 2,657,912 to 2,658,424
_RS12390
RR42_RS12395: RR42_RS12395 - hypothetical protein, at 2,658,656 to 2,659,798
_RS12395
Position (kb)
2657
2658
2659 Strain fitness (log2 ratio)
-2
-1
0
1 at 2656.967 kb on + strand, within RR42_RS12385 at 2656.968 kb on - strand, within RR42_RS12385 at 2656.968 kb on - strand, within RR42_RS12385 at 2657.029 kb on + strand, within RR42_RS12385 at 2657.029 kb on + strand, within RR42_RS12385 at 2657.029 kb on + strand, within RR42_RS12385 at 2657.029 kb on + strand, within RR42_RS12385 at 2657.030 kb on - strand, within RR42_RS12385 at 2657.030 kb on - strand, within RR42_RS12385 at 2657.042 kb on - strand, within RR42_RS12385 at 2657.042 kb on - strand, within RR42_RS12385 at 2657.042 kb on - strand, within RR42_RS12385 at 2657.109 kb on - strand, within RR42_RS12385 at 2657.109 kb on - strand, within RR42_RS12385 at 2657.252 kb on + strand, within RR42_RS12385 at 2657.253 kb on - strand, within RR42_RS12385 at 2657.253 kb on - strand, within RR42_RS12385 at 2657.344 kb on + strand, within RR42_RS12385 at 2657.345 kb on - strand, within RR42_RS12385 at 2657.466 kb on + strand, within RR42_RS12385 at 2657.614 kb on + strand, within RR42_RS12385 at 2657.615 kb on - strand, within RR42_RS12385 at 2657.615 kb on - strand, within RR42_RS12385 at 2657.913 kb on + strand at 2657.914 kb on - strand at 2657.914 kb on - strand at 2658.033 kb on + strand, within RR42_RS12390 at 2658.083 kb on - strand, within RR42_RS12390 at 2658.214 kb on - strand, within RR42_RS12390 at 2658.398 kb on + strand at 2658.402 kb on + strand at 2658.402 kb on + strand at 2658.419 kb on + strand at 2658.429 kb on + strand at 2658.429 kb on + strand at 2658.430 kb on - strand at 2658.511 kb on + strand at 2658.511 kb on + strand at 2658.511 kb on + strand at 2658.512 kb on - strand at 2658.512 kb on - strand at 2658.512 kb on - strand at 2658.513 kb on + strand at 2658.513 kb on + strand at 2658.514 kb on - strand at 2658.514 kb on - strand at 2658.774 kb on - strand, within RR42_RS12395 at 2658.774 kb on - strand, within RR42_RS12395 at 2659.161 kb on + strand, within RR42_RS12395 at 2659.161 kb on + strand, within RR42_RS12395 at 2659.162 kb on - strand, within RR42_RS12395 at 2659.162 kb on - strand, within RR42_RS12395 at 2659.177 kb on - strand, within RR42_RS12395 at 2659.264 kb on + strand, within RR42_RS12395 at 2659.264 kb on + strand, within RR42_RS12395 at 2659.264 kb on + strand, within RR42_RS12395 at 2659.351 kb on + strand, within RR42_RS12395
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,656,967 + RR42_RS12385 0.37 -2.6 2,656,968 - RR42_RS12385 0.37 +0.7 2,656,968 - RR42_RS12385 0.37 +0.2 2,657,029 + RR42_RS12385 0.42 -2.3 2,657,029 + RR42_RS12385 0.42 +0.3 2,657,029 + RR42_RS12385 0.42 +0.6 2,657,029 + RR42_RS12385 0.42 -1.2 2,657,030 - RR42_RS12385 0.42 -0.1 2,657,030 - RR42_RS12385 0.42 +1.2 2,657,042 - RR42_RS12385 0.43 -1.0 2,657,042 - RR42_RS12385 0.43 +0.4 2,657,042 - RR42_RS12385 0.43 -0.5 2,657,109 - RR42_RS12385 0.48 -0.8 2,657,109 - RR42_RS12385 0.48 +0.6 2,657,252 + RR42_RS12385 0.60 +0.3 2,657,253 - RR42_RS12385 0.60 -2.0 2,657,253 - RR42_RS12385 0.60 +0.4 2,657,344 + RR42_RS12385 0.67 -0.7 2,657,345 - RR42_RS12385 0.67 -0.7 2,657,466 + RR42_RS12385 0.77 +0.1 2,657,614 + RR42_RS12385 0.89 -0.8 2,657,615 - RR42_RS12385 0.89 +1.7 2,657,615 - RR42_RS12385 0.89 +0.0 2,657,913 + +0.6 2,657,914 - -0.3 2,657,914 - +0.5 2,658,033 + RR42_RS12390 0.24 -2.4 2,658,083 - RR42_RS12390 0.33 -0.4 2,658,214 - RR42_RS12390 0.59 -0.7 2,658,398 + -0.2 2,658,402 + -1.1 2,658,402 + +0.0 2,658,419 + +0.1 2,658,429 + -1.0 2,658,429 + +0.4 2,658,430 - +0.8 2,658,511 + +0.2 2,658,511 + +0.4 2,658,511 + -0.6 2,658,512 - -0.3 2,658,512 - -0.5 2,658,512 - +1.3 2,658,513 + -1.4 2,658,513 + -0.1 2,658,514 - -0.5 2,658,514 - -0.1 2,658,774 - RR42_RS12395 0.10 +0.8 2,658,774 - RR42_RS12395 0.10 -1.0 2,659,161 + RR42_RS12395 0.44 +0.1 2,659,161 + RR42_RS12395 0.44 +0.5 2,659,162 - RR42_RS12395 0.44 +0.5 2,659,162 - RR42_RS12395 0.44 +0.7 2,659,177 - RR42_RS12395 0.46 +0.5 2,659,264 + RR42_RS12395 0.53 -1.7 2,659,264 + RR42_RS12395 0.53 +0.4 2,659,264 + RR42_RS12395 0.53 -0.4 2,659,351 + RR42_RS12395 0.61 -0.0
Or see this region's nucleotide sequence