Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11620 and RR42_RS11625 are separated by 152 nucleotides RR42_RS11625 and RR42_RS11630 are separated by 133 nucleotides
RR42_RS11620: RR42_RS11620 - polysaccharide deacetylase, at 2,488,356 to 2,489,111
_RS11620
RR42_RS11625: RR42_RS11625 - hypothetical protein, at 2,489,264 to 2,490,613
_RS11625
RR42_RS11630: RR42_RS11630 - magnesium ABC transporter ATPase, at 2,490,747 to 2,493,329
_RS11630
Position (kb)
2489
2490
2491 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2488.292 kb on + strand at 2488.292 kb on + strand at 2488.292 kb on + strand at 2488.293 kb on - strand at 2488.510 kb on + strand, within RR42_RS11620 at 2488.729 kb on + strand, within RR42_RS11620 at 2488.760 kb on + strand, within RR42_RS11620 at 2488.761 kb on - strand, within RR42_RS11620 at 2488.761 kb on - strand, within RR42_RS11620 at 2489.011 kb on + strand, within RR42_RS11620 at 2489.011 kb on + strand, within RR42_RS11620 at 2489.012 kb on - strand, within RR42_RS11620 at 2489.092 kb on - strand at 2489.117 kb on - strand at 2489.245 kb on - strand at 2489.250 kb on + strand at 2489.265 kb on + strand at 2489.266 kb on - strand at 2489.358 kb on - strand at 2489.669 kb on + strand, within RR42_RS11625 at 2489.669 kb on + strand, within RR42_RS11625 at 2489.670 kb on - strand, within RR42_RS11625 at 2489.796 kb on + strand, within RR42_RS11625 at 2489.796 kb on + strand, within RR42_RS11625 at 2489.797 kb on - strand, within RR42_RS11625 at 2489.797 kb on - strand, within RR42_RS11625 at 2489.837 kb on + strand, within RR42_RS11625 at 2489.837 kb on + strand, within RR42_RS11625 at 2489.838 kb on - strand, within RR42_RS11625 at 2489.838 kb on - strand, within RR42_RS11625 at 2489.856 kb on - strand, within RR42_RS11625 at 2490.031 kb on - strand, within RR42_RS11625 at 2490.031 kb on - strand, within RR42_RS11625 at 2490.074 kb on + strand, within RR42_RS11625 at 2490.074 kb on + strand, within RR42_RS11625 at 2490.075 kb on - strand, within RR42_RS11625 at 2490.076 kb on - strand, within RR42_RS11625 at 2490.270 kb on + strand, within RR42_RS11625 at 2490.271 kb on - strand, within RR42_RS11625 at 2490.271 kb on - strand, within RR42_RS11625 at 2490.370 kb on - strand, within RR42_RS11625 at 2490.540 kb on + strand at 2490.540 kb on + strand at 2490.541 kb on - strand at 2490.561 kb on - strand at 2490.658 kb on + strand at 2490.659 kb on - strand at 2490.680 kb on + strand at 2490.681 kb on - strand at 2490.681 kb on - strand at 2490.718 kb on + strand at 2490.740 kb on + strand at 2490.790 kb on + strand at 2490.791 kb on - strand at 2490.791 kb on - strand at 2490.887 kb on - strand at 2491.102 kb on + strand, within RR42_RS11630 at 2491.139 kb on + strand, within RR42_RS11630 at 2491.139 kb on + strand, within RR42_RS11630 at 2491.139 kb on + strand, within RR42_RS11630 at 2491.140 kb on - strand, within RR42_RS11630 at 2491.140 kb on - strand, within RR42_RS11630 at 2491.141 kb on + strand, within RR42_RS11630 at 2491.142 kb on - strand, within RR42_RS11630 at 2491.305 kb on + strand, within RR42_RS11630 at 2491.316 kb on + strand, within RR42_RS11630 at 2491.316 kb on + strand, within RR42_RS11630 at 2491.316 kb on + strand, within RR42_RS11630 at 2491.317 kb on - strand, within RR42_RS11630 at 2491.351 kb on + strand, within RR42_RS11630 at 2491.352 kb on - strand, within RR42_RS11630 at 2491.352 kb on - strand, within RR42_RS11630 at 2491.377 kb on - strand, within RR42_RS11630
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,488,292 + -0.0 2,488,292 + +0.1 2,488,292 + +0.4 2,488,293 - -0.1 2,488,510 + RR42_RS11620 0.20 +0.0 2,488,729 + RR42_RS11620 0.49 -0.4 2,488,760 + RR42_RS11620 0.53 +0.4 2,488,761 - RR42_RS11620 0.54 +0.6 2,488,761 - RR42_RS11620 0.54 +0.3 2,489,011 + RR42_RS11620 0.87 -1.3 2,489,011 + RR42_RS11620 0.87 -0.1 2,489,012 - RR42_RS11620 0.87 +0.2 2,489,092 - -0.8 2,489,117 - +0.6 2,489,245 - -0.3 2,489,250 + +0.6 2,489,265 + -1.3 2,489,266 - -0.0 2,489,358 - -0.1 2,489,669 + RR42_RS11625 0.30 -1.5 2,489,669 + RR42_RS11625 0.30 +0.5 2,489,670 - RR42_RS11625 0.30 +1.9 2,489,796 + RR42_RS11625 0.39 +0.1 2,489,796 + RR42_RS11625 0.39 -0.3 2,489,797 - RR42_RS11625 0.39 -0.1 2,489,797 - RR42_RS11625 0.39 +0.5 2,489,837 + RR42_RS11625 0.42 -0.1 2,489,837 + RR42_RS11625 0.42 +1.5 2,489,838 - RR42_RS11625 0.43 +0.7 2,489,838 - RR42_RS11625 0.43 -1.8 2,489,856 - RR42_RS11625 0.44 -0.1 2,490,031 - RR42_RS11625 0.57 -0.1 2,490,031 - RR42_RS11625 0.57 -0.1 2,490,074 + RR42_RS11625 0.60 +0.9 2,490,074 + RR42_RS11625 0.60 +0.5 2,490,075 - RR42_RS11625 0.60 -0.8 2,490,076 - RR42_RS11625 0.60 +0.7 2,490,270 + RR42_RS11625 0.75 +0.5 2,490,271 - RR42_RS11625 0.75 +0.4 2,490,271 - RR42_RS11625 0.75 -0.3 2,490,370 - RR42_RS11625 0.82 +0.1 2,490,540 + +0.2 2,490,540 + +0.1 2,490,541 - -0.0 2,490,561 - +0.9 2,490,658 + +0.1 2,490,659 - +0.5 2,490,680 + +0.0 2,490,681 - -0.4 2,490,681 - +0.7 2,490,718 + -0.8 2,490,740 + -0.5 2,490,790 + -0.5 2,490,791 - +1.7 2,490,791 - +0.0 2,490,887 - -1.3 2,491,102 + RR42_RS11630 0.14 +0.2 2,491,139 + RR42_RS11630 0.15 +0.7 2,491,139 + RR42_RS11630 0.15 -0.5 2,491,139 + RR42_RS11630 0.15 -0.1 2,491,140 - RR42_RS11630 0.15 +0.3 2,491,140 - RR42_RS11630 0.15 +1.4 2,491,141 + RR42_RS11630 0.15 -0.4 2,491,142 - RR42_RS11630 0.15 +0.3 2,491,305 + RR42_RS11630 0.22 -2.8 2,491,316 + RR42_RS11630 0.22 -0.1 2,491,316 + RR42_RS11630 0.22 -0.7 2,491,316 + RR42_RS11630 0.22 -0.1 2,491,317 - RR42_RS11630 0.22 +0.7 2,491,351 + RR42_RS11630 0.23 +0.1 2,491,352 - RR42_RS11630 0.23 +0.3 2,491,352 - RR42_RS11630 0.23 -0.5 2,491,377 - RR42_RS11630 0.24 +0.1
Or see this region's nucleotide sequence