Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11560 and RR42_RS11565 are separated by 675 nucleotides RR42_RS11565 and RR42_RS11570 are separated by 601 nucleotides
RR42_RS11560: RR42_RS11560 - protein involved in RimO-mediated beta-methylthiolation of ribosomal protein S12 YcaO, at 2,472,357 to 2,474,561
_RS11560
RR42_RS11565: RR42_RS11565 - diguanylate cyclase, at 2,475,237 to 2,477,711
_RS11565
RR42_RS11570: RR42_RS11570 - magnesium transporter MgtC, at 2,478,313 to 2,478,633
_RS11570
Position (kb)
2475
2476
2477
2478 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2474.382 kb on + strand at 2474.389 kb on + strand at 2474.389 kb on + strand at 2474.390 kb on - strand at 2474.471 kb on + strand at 2474.534 kb on + strand at 2474.534 kb on + strand at 2474.535 kb on - strand at 2474.671 kb on - strand at 2474.751 kb on - strand at 2474.996 kb on - strand at 2475.131 kb on - strand at 2475.347 kb on + strand at 2475.482 kb on - strand at 2475.482 kb on - strand at 2475.496 kb on + strand, within RR42_RS11565 at 2475.496 kb on + strand, within RR42_RS11565 at 2475.910 kb on + strand, within RR42_RS11565 at 2475.910 kb on + strand, within RR42_RS11565 at 2475.910 kb on + strand, within RR42_RS11565 at 2475.911 kb on - strand, within RR42_RS11565 at 2475.911 kb on - strand, within RR42_RS11565 at 2475.911 kb on - strand, within RR42_RS11565 at 2475.923 kb on - strand, within RR42_RS11565 at 2476.115 kb on + strand, within RR42_RS11565 at 2476.115 kb on + strand, within RR42_RS11565 at 2476.164 kb on + strand, within RR42_RS11565 at 2476.242 kb on + strand, within RR42_RS11565 at 2476.327 kb on - strand, within RR42_RS11565 at 2476.327 kb on - strand, within RR42_RS11565 at 2476.327 kb on - strand, within RR42_RS11565 at 2476.386 kb on + strand, within RR42_RS11565 at 2476.386 kb on + strand, within RR42_RS11565 at 2476.387 kb on - strand, within RR42_RS11565 at 2476.387 kb on - strand, within RR42_RS11565 at 2476.387 kb on - strand, within RR42_RS11565 at 2476.390 kb on + strand, within RR42_RS11565 at 2476.391 kb on - strand, within RR42_RS11565 at 2476.458 kb on + strand, within RR42_RS11565 at 2476.552 kb on - strand, within RR42_RS11565 at 2476.561 kb on + strand, within RR42_RS11565 at 2476.569 kb on + strand, within RR42_RS11565 at 2476.570 kb on - strand, within RR42_RS11565 at 2476.570 kb on - strand, within RR42_RS11565 at 2476.599 kb on - strand, within RR42_RS11565 at 2476.766 kb on + strand, within RR42_RS11565 at 2476.817 kb on - strand, within RR42_RS11565 at 2476.817 kb on - strand, within RR42_RS11565 at 2476.942 kb on + strand, within RR42_RS11565 at 2476.943 kb on - strand, within RR42_RS11565 at 2477.030 kb on + strand, within RR42_RS11565 at 2477.045 kb on + strand, within RR42_RS11565 at 2477.045 kb on + strand, within RR42_RS11565 at 2477.046 kb on - strand, within RR42_RS11565 at 2477.048 kb on - strand, within RR42_RS11565 at 2477.062 kb on + strand, within RR42_RS11565 at 2477.063 kb on - strand, within RR42_RS11565 at 2477.259 kb on - strand, within RR42_RS11565 at 2477.372 kb on + strand, within RR42_RS11565 at 2477.450 kb on + strand, within RR42_RS11565 at 2477.450 kb on + strand, within RR42_RS11565 at 2477.451 kb on - strand, within RR42_RS11565 at 2477.451 kb on - strand, within RR42_RS11565 at 2477.532 kb on + strand at 2477.533 kb on - strand at 2477.695 kb on + strand at 2477.695 kb on + strand at 2477.695 kb on + strand at 2477.695 kb on + strand at 2477.696 kb on - strand at 2477.696 kb on - strand at 2477.707 kb on + strand at 2477.752 kb on - strand at 2478.000 kb on - strand at 2478.000 kb on - strand at 2478.012 kb on + strand at 2478.013 kb on - strand at 2478.250 kb on + strand at 2478.625 kb on + strand at 2478.694 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,474,382 + -0.1 2,474,389 + -0.3 2,474,389 + +0.3 2,474,390 - +3.1 2,474,471 + +0.2 2,474,534 + +0.4 2,474,534 + -0.2 2,474,535 - -1.2 2,474,671 - -0.1 2,474,751 - -0.0 2,474,996 - -0.6 2,475,131 - +0.6 2,475,347 + +0.5 2,475,482 - -0.1 2,475,482 - +0.1 2,475,496 + RR42_RS11565 0.10 -0.5 2,475,496 + RR42_RS11565 0.10 -1.0 2,475,910 + RR42_RS11565 0.27 +0.8 2,475,910 + RR42_RS11565 0.27 +0.6 2,475,910 + RR42_RS11565 0.27 +0.0 2,475,911 - RR42_RS11565 0.27 -0.7 2,475,911 - RR42_RS11565 0.27 +0.2 2,475,911 - RR42_RS11565 0.27 +0.4 2,475,923 - RR42_RS11565 0.28 -0.2 2,476,115 + RR42_RS11565 0.35 -1.8 2,476,115 + RR42_RS11565 0.35 +1.4 2,476,164 + RR42_RS11565 0.37 +1.2 2,476,242 + RR42_RS11565 0.41 -0.1 2,476,327 - RR42_RS11565 0.44 -0.7 2,476,327 - RR42_RS11565 0.44 +1.2 2,476,327 - RR42_RS11565 0.44 -0.1 2,476,386 + RR42_RS11565 0.46 -1.2 2,476,386 + RR42_RS11565 0.46 +0.1 2,476,387 - RR42_RS11565 0.46 +0.4 2,476,387 - RR42_RS11565 0.46 -1.4 2,476,387 - RR42_RS11565 0.46 -1.0 2,476,390 + RR42_RS11565 0.47 +0.2 2,476,391 - RR42_RS11565 0.47 +0.4 2,476,458 + RR42_RS11565 0.49 +0.1 2,476,552 - RR42_RS11565 0.53 +0.0 2,476,561 + RR42_RS11565 0.53 -0.4 2,476,569 + RR42_RS11565 0.54 +1.4 2,476,570 - RR42_RS11565 0.54 +0.5 2,476,570 - RR42_RS11565 0.54 -1.0 2,476,599 - RR42_RS11565 0.55 -0.2 2,476,766 + RR42_RS11565 0.62 -0.7 2,476,817 - RR42_RS11565 0.64 +0.3 2,476,817 - RR42_RS11565 0.64 -0.1 2,476,942 + RR42_RS11565 0.69 -0.1 2,476,943 - RR42_RS11565 0.69 +0.1 2,477,030 + RR42_RS11565 0.72 +0.8 2,477,045 + RR42_RS11565 0.73 +0.5 2,477,045 + RR42_RS11565 0.73 -0.9 2,477,046 - RR42_RS11565 0.73 -1.1 2,477,048 - RR42_RS11565 0.73 +0.3 2,477,062 + RR42_RS11565 0.74 -0.1 2,477,063 - RR42_RS11565 0.74 -0.6 2,477,259 - RR42_RS11565 0.82 +0.1 2,477,372 + RR42_RS11565 0.86 +0.2 2,477,450 + RR42_RS11565 0.89 +1.0 2,477,450 + RR42_RS11565 0.89 +0.2 2,477,451 - RR42_RS11565 0.89 +0.4 2,477,451 - RR42_RS11565 0.89 +0.3 2,477,532 + +0.3 2,477,533 - +0.8 2,477,695 + -0.1 2,477,695 + -0.1 2,477,695 + +1.9 2,477,695 + -0.2 2,477,696 - +0.6 2,477,696 - +0.4 2,477,707 + -0.3 2,477,752 - +0.9 2,478,000 - +0.4 2,478,000 - -1.2 2,478,012 + -0.7 2,478,013 - -2.5 2,478,250 + +0.7 2,478,625 + -0.3 2,478,694 + +0.1
Or see this region's nucleotide sequence