Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10890 and RR42_RS10895 are separated by 82 nucleotides RR42_RS10895 and RR42_RS10900 are separated by 118 nucleotides RR42_RS10900 and RR42_RS10905 are separated by 123 nucleotides RR42_RS10905 and RR42_RS10910 are separated by 8 nucleotides
RR42_RS10890: RR42_RS10890 - phosphinothricin acetyltransferase, at 2,330,435 to 2,330,956
_RS10890
RR42_RS10895: RR42_RS10895 - pilus assembly protein, at 2,331,039 to 2,331,422
_RS10895
RR42_RS10900: RR42_RS10900 - DNA polymerase III subunit delta', at 2,331,541 to 2,332,548
_RS10900
RR42_RS10905: RR42_RS10905 - thymidylate kinase, at 2,332,672 to 2,333,286
_RS10905
RR42_RS10910: RR42_RS10910 - aminodeoxychorismate lyase, at 2,333,295 to 2,334,290
_RS10910
Position (kb)
2331
2332
2333 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2330.596 kb on + strand, within RR42_RS10890 at 2330.596 kb on + strand, within RR42_RS10890 at 2330.596 kb on + strand, within RR42_RS10890 at 2330.596 kb on + strand, within RR42_RS10890 at 2330.596 kb on + strand, within RR42_RS10890 at 2330.597 kb on - strand, within RR42_RS10890 at 2330.597 kb on - strand, within RR42_RS10890 at 2330.643 kb on + strand, within RR42_RS10890 at 2330.643 kb on + strand, within RR42_RS10890 at 2330.643 kb on + strand, within RR42_RS10890 at 2330.643 kb on + strand, within RR42_RS10890 at 2330.643 kb on + strand, within RR42_RS10890 at 2330.644 kb on - strand, within RR42_RS10890 at 2330.682 kb on + strand, within RR42_RS10890 at 2330.682 kb on + strand, within RR42_RS10890 at 2330.889 kb on + strand, within RR42_RS10890 at 2330.889 kb on + strand, within RR42_RS10890 at 2330.890 kb on - strand, within RR42_RS10890 at 2330.890 kb on - strand, within RR42_RS10890 at 2330.890 kb on - strand, within RR42_RS10890 at 2330.890 kb on - strand, within RR42_RS10890 at 2330.938 kb on + strand at 2331.014 kb on - strand at 2331.017 kb on + strand at 2331.017 kb on + strand at 2331.018 kb on - strand at 2331.018 kb on - strand at 2331.208 kb on + strand, within RR42_RS10895 at 2331.208 kb on + strand, within RR42_RS10895 at 2331.209 kb on - strand, within RR42_RS10895 at 2331.316 kb on + strand, within RR42_RS10895 at 2331.316 kb on + strand, within RR42_RS10895 at 2331.316 kb on + strand, within RR42_RS10895 at 2331.316 kb on + strand, within RR42_RS10895 at 2331.317 kb on - strand, within RR42_RS10895 at 2331.317 kb on - strand, within RR42_RS10895 at 2331.317 kb on - strand, within RR42_RS10895 at 2331.317 kb on - strand, within RR42_RS10895 at 2331.325 kb on + strand, within RR42_RS10895 at 2331.326 kb on - strand, within RR42_RS10895 at 2331.326 kb on - strand, within RR42_RS10895 at 2331.470 kb on + strand at 2331.470 kb on + strand at 2331.470 kb on + strand at 2331.470 kb on + strand at 2331.471 kb on - strand at 2331.471 kb on - strand at 2331.471 kb on - strand at 2331.492 kb on - strand at 2331.492 kb on - strand at 2331.492 kb on - strand at 2331.542 kb on + strand at 2331.542 kb on + strand at 2331.543 kb on - strand at 2332.542 kb on - strand at 2332.564 kb on - strand at 2332.564 kb on - strand at 2332.582 kb on - strand at 2332.586 kb on - strand at 2332.586 kb on - strand at 2332.586 kb on - strand at 2332.586 kb on - strand at 2332.635 kb on - strand at 2333.297 kb on - strand at 2333.297 kb on - strand at 2333.387 kb on - strand at 2333.484 kb on - strand, within RR42_RS10910 at 2333.484 kb on - strand, within RR42_RS10910 at 2333.484 kb on - strand, within RR42_RS10910 at 2333.484 kb on - strand, within RR42_RS10910 at 2333.484 kb on - strand, within RR42_RS10910 at 2333.488 kb on + strand, within RR42_RS10910 at 2333.488 kb on + strand, within RR42_RS10910 at 2333.488 kb on + strand, within RR42_RS10910 at 2333.489 kb on - strand, within RR42_RS10910 at 2333.489 kb on - strand, within RR42_RS10910 at 2333.489 kb on - strand, within RR42_RS10910 at 2333.489 kb on - strand, within RR42_RS10910
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,330,596 + RR42_RS10890 0.31 -1.0 2,330,596 + RR42_RS10890 0.31 -0.2 2,330,596 + RR42_RS10890 0.31 -0.8 2,330,596 + RR42_RS10890 0.31 +0.0 2,330,596 + RR42_RS10890 0.31 -0.2 2,330,597 - RR42_RS10890 0.31 +0.2 2,330,597 - RR42_RS10890 0.31 -0.4 2,330,643 + RR42_RS10890 0.40 -0.8 2,330,643 + RR42_RS10890 0.40 -0.3 2,330,643 + RR42_RS10890 0.40 -0.7 2,330,643 + RR42_RS10890 0.40 +0.9 2,330,643 + RR42_RS10890 0.40 -0.6 2,330,644 - RR42_RS10890 0.40 -1.4 2,330,682 + RR42_RS10890 0.47 -0.4 2,330,682 + RR42_RS10890 0.47 -0.1 2,330,889 + RR42_RS10890 0.87 +0.2 2,330,889 + RR42_RS10890 0.87 -0.6 2,330,890 - RR42_RS10890 0.87 +0.1 2,330,890 - RR42_RS10890 0.87 -0.3 2,330,890 - RR42_RS10890 0.87 -0.3 2,330,890 - RR42_RS10890 0.87 -0.4 2,330,938 + +0.4 2,331,014 - -0.5 2,331,017 + -0.5 2,331,017 + -0.2 2,331,018 - -0.4 2,331,018 - -0.0 2,331,208 + RR42_RS10895 0.44 +0.8 2,331,208 + RR42_RS10895 0.44 -1.3 2,331,209 - RR42_RS10895 0.44 +0.7 2,331,316 + RR42_RS10895 0.72 +0.4 2,331,316 + RR42_RS10895 0.72 +0.7 2,331,316 + RR42_RS10895 0.72 +0.5 2,331,316 + RR42_RS10895 0.72 -0.8 2,331,317 - RR42_RS10895 0.72 -0.6 2,331,317 - RR42_RS10895 0.72 +0.3 2,331,317 - RR42_RS10895 0.72 -0.7 2,331,317 - RR42_RS10895 0.72 -1.7 2,331,325 + RR42_RS10895 0.74 +0.7 2,331,326 - RR42_RS10895 0.75 -1.7 2,331,326 - RR42_RS10895 0.75 +0.5 2,331,470 + -1.5 2,331,470 + +0.2 2,331,470 + +0.2 2,331,470 + -0.5 2,331,471 - +0.3 2,331,471 - -0.2 2,331,471 - -0.7 2,331,492 - -0.2 2,331,492 - -0.5 2,331,492 - -0.1 2,331,542 + +0.4 2,331,542 + -0.3 2,331,543 - -1.1 2,332,542 - -1.5 2,332,564 - -2.9 2,332,564 - +0.2 2,332,582 - -0.5 2,332,586 - +0.3 2,332,586 - -0.5 2,332,586 - +0.7 2,332,586 - -1.0 2,332,635 - -0.8 2,333,297 - +0.3 2,333,297 - -3.6 2,333,387 - -0.2 2,333,484 - RR42_RS10910 0.19 +0.7 2,333,484 - RR42_RS10910 0.19 -0.1 2,333,484 - RR42_RS10910 0.19 -0.3 2,333,484 - RR42_RS10910 0.19 -0.3 2,333,484 - RR42_RS10910 0.19 -0.5 2,333,488 + RR42_RS10910 0.19 +0.0 2,333,488 + RR42_RS10910 0.19 -1.1 2,333,488 + RR42_RS10910 0.19 +0.4 2,333,489 - RR42_RS10910 0.19 -0.6 2,333,489 - RR42_RS10910 0.19 +0.0 2,333,489 - RR42_RS10910 0.19 -0.8 2,333,489 - RR42_RS10910 0.19 -0.6
Or see this region's nucleotide sequence