Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09570 and RR42_RS09575 are separated by 198 nucleotides RR42_RS09575 and RR42_RS09580 overlap by 4 nucleotides RR42_RS09580 and RR42_RS09585 are separated by 268 nucleotides
RR42_RS09570: RR42_RS09570 - hypothetical protein, at 2,026,989 to 2,028,572
_RS09570
RR42_RS09575: RR42_RS09575 - hypothetical protein, at 2,028,771 to 2,029,058
_RS09575
RR42_RS09580: RR42_RS09580 - hypothetical protein, at 2,029,055 to 2,029,336
_RS09580
RR42_RS09585: RR42_RS09585 - Crp/Fnr family transcriptional regulator, at 2,029,605 to 2,030,363
_RS09585
Position (kb)
2029
2030 Strain fitness (log2 ratio)
-1
0
1
2 at 2028.338 kb on - strand, within RR42_RS09570 at 2028.451 kb on + strand at 2028.452 kb on - strand at 2028.452 kb on - strand at 2028.452 kb on - strand at 2028.627 kb on + strand at 2028.628 kb on - strand at 2028.628 kb on - strand at 2028.686 kb on - strand at 2028.686 kb on - strand at 2028.688 kb on - strand at 2028.688 kb on - strand at 2028.688 kb on - strand at 2028.688 kb on - strand at 2028.814 kb on + strand, within RR42_RS09575 at 2028.814 kb on + strand, within RR42_RS09575 at 2028.814 kb on + strand, within RR42_RS09575 at 2028.814 kb on + strand, within RR42_RS09575 at 2028.815 kb on - strand, within RR42_RS09575 at 2029.056 kb on + strand at 2029.057 kb on - strand at 2029.237 kb on - strand, within RR42_RS09580 at 2029.460 kb on - strand at 2029.727 kb on - strand, within RR42_RS09585 at 2029.805 kb on + strand, within RR42_RS09585 at 2030.128 kb on + strand, within RR42_RS09585 at 2030.129 kb on - strand, within RR42_RS09585 at 2030.129 kb on - strand, within RR42_RS09585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 2,028,338 - RR42_RS09570 0.85 +0.4 2,028,451 + +0.1 2,028,452 - -0.5 2,028,452 - +2.0 2,028,452 - +0.2 2,028,627 + +0.4 2,028,628 - +0.3 2,028,628 - -0.4 2,028,686 - +0.3 2,028,686 - -0.2 2,028,688 - -1.0 2,028,688 - +0.5 2,028,688 - +0.0 2,028,688 - -0.1 2,028,814 + RR42_RS09575 0.15 -0.0 2,028,814 + RR42_RS09575 0.15 -0.0 2,028,814 + RR42_RS09575 0.15 -0.4 2,028,814 + RR42_RS09575 0.15 -0.2 2,028,815 - RR42_RS09575 0.15 +0.8 2,029,056 + -1.0 2,029,057 - -1.1 2,029,237 - RR42_RS09580 0.65 -0.7 2,029,460 - +0.6 2,029,727 - RR42_RS09585 0.16 +0.3 2,029,805 + RR42_RS09585 0.26 -0.1 2,030,128 + RR42_RS09585 0.69 -0.2 2,030,129 - RR42_RS09585 0.69 +0.8 2,030,129 - RR42_RS09585 0.69 +1.0
Or see this region's nucleotide sequence