Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09010 and RR42_RS09015 are separated by 226 nucleotides RR42_RS09015 and RR42_RS09020 are separated by 73 nucleotides RR42_RS09020 and RR42_RS09025 are separated by 246 nucleotides
RR42_RS09010: RR42_RS09010 - FAD-dependent cmnm(5)s(2)U34 oxidoreductase, at 1,891,929 to 1,893,953
_RS09010
RR42_RS09015: RR42_RS09015 - trigger factor, at 1,894,180 to 1,895,541
_RS09015
RR42_RS09020: RR42_RS09020 - Clp protease ClpP, at 1,895,615 to 1,896,265
_RS09020
RR42_RS09025: RR42_RS09025 - Clp protease ATP-binding protein, at 1,896,512 to 1,897,786
_RS09025
Position (kb)
1894
1895
1896 Strain fitness (log2 ratio)
-2
-1
0
1 at 1893.297 kb on + strand, within RR42_RS09010 at 1893.329 kb on + strand, within RR42_RS09010 at 1893.329 kb on + strand, within RR42_RS09010 at 1893.330 kb on - strand, within RR42_RS09010 at 1893.330 kb on - strand, within RR42_RS09010 at 1893.477 kb on + strand, within RR42_RS09010 at 1893.477 kb on + strand, within RR42_RS09010 at 1893.616 kb on - strand, within RR42_RS09010 at 1893.765 kb on + strand at 1893.765 kb on + strand at 1893.766 kb on - strand at 1893.766 kb on - strand at 1893.766 kb on - strand at 1894.633 kb on + strand, within RR42_RS09015 at 1894.633 kb on + strand, within RR42_RS09015 at 1895.356 kb on + strand, within RR42_RS09015 at 1895.389 kb on + strand, within RR42_RS09015 at 1895.425 kb on + strand at 1895.867 kb on + strand, within RR42_RS09020 at 1895.912 kb on + strand, within RR42_RS09020 at 1896.354 kb on + strand at 1896.356 kb on + strand at 1896.456 kb on + strand at 1896.495 kb on + strand at 1896.495 kb on + strand at 1896.495 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 1,893,297 + RR42_RS09010 0.68 +0.2 1,893,329 + RR42_RS09010 0.69 -1.4 1,893,329 + RR42_RS09010 0.69 +0.5 1,893,330 - RR42_RS09010 0.69 +1.0 1,893,330 - RR42_RS09010 0.69 -0.3 1,893,477 + RR42_RS09010 0.76 +0.8 1,893,477 + RR42_RS09010 0.76 -0.4 1,893,616 - RR42_RS09010 0.83 +0.1 1,893,765 + +0.1 1,893,765 + -0.2 1,893,766 - -0.4 1,893,766 - +0.5 1,893,766 - -1.8 1,894,633 + RR42_RS09015 0.33 -2.2 1,894,633 + RR42_RS09015 0.33 -1.3 1,895,356 + RR42_RS09015 0.86 +0.2 1,895,389 + RR42_RS09015 0.89 -0.1 1,895,425 + +0.8 1,895,867 + RR42_RS09020 0.39 -0.3 1,895,912 + RR42_RS09020 0.46 +0.4 1,896,354 + +0.4 1,896,356 + -0.4 1,896,456 + +0.1 1,896,495 + +0.4 1,896,495 + -0.4 1,896,495 + +0.2
Or see this region's nucleotide sequence