Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08015 and RR42_RS08020 overlap by 2 nucleotides RR42_RS08020 and RR42_RS08025 are separated by 32 nucleotides RR42_RS08025 and RR42_RS08030 are separated by 35 nucleotides RR42_RS08030 and RR42_RS08035 are separated by 2 nucleotides
RR42_RS08015: RR42_RS08015 - hypothetical protein, at 1,719,637 to 1,720,380
_RS08015
RR42_RS08020: RR42_RS08020 - hypothetical protein, at 1,720,379 to 1,720,918
_RS08020
RR42_RS08025: RR42_RS08025 - phage Gp37Gp68, at 1,720,951 to 1,721,838
_RS08025
RR42_RS08030: RR42_RS08030 - hypothetical protein, at 1,721,874 to 1,722,170
_RS08030
RR42_RS08035: RR42_RS08035 - DNA methyltransferase, at 1,722,173 to 1,723,957
_RS08035
Position (kb)
1720
1721
1722 Strain fitness (log2 ratio)
-1
0
1 at 1720.004 kb on + strand, within RR42_RS08015 at 1720.154 kb on + strand, within RR42_RS08015 at 1720.154 kb on + strand, within RR42_RS08015 at 1720.154 kb on + strand, within RR42_RS08015 at 1720.154 kb on + strand, within RR42_RS08015 at 1720.278 kb on + strand, within RR42_RS08015 at 1720.279 kb on - strand, within RR42_RS08015 at 1720.279 kb on - strand, within RR42_RS08015 at 1720.342 kb on + strand at 1720.342 kb on + strand at 1720.342 kb on + strand at 1720.383 kb on - strand at 1720.621 kb on + strand, within RR42_RS08020 at 1720.625 kb on + strand, within RR42_RS08020 at 1720.770 kb on + strand, within RR42_RS08020 at 1720.801 kb on + strand, within RR42_RS08020 at 1720.801 kb on + strand, within RR42_RS08020 at 1720.809 kb on + strand, within RR42_RS08020 at 1720.809 kb on + strand, within RR42_RS08020 at 1720.809 kb on + strand, within RR42_RS08020 at 1721.399 kb on + strand, within RR42_RS08025 at 1721.616 kb on + strand, within RR42_RS08025 at 1721.971 kb on + strand, within RR42_RS08030 at 1722.105 kb on + strand, within RR42_RS08030 at 1722.256 kb on + strand at 1722.256 kb on + strand at 1722.384 kb on + strand, within RR42_RS08035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 1,720,004 + RR42_RS08015 0.49 -1.3 1,720,154 + RR42_RS08015 0.69 +0.2 1,720,154 + RR42_RS08015 0.69 -0.1 1,720,154 + RR42_RS08015 0.69 +0.4 1,720,154 + RR42_RS08015 0.69 -0.3 1,720,278 + RR42_RS08015 0.86 -0.4 1,720,279 - RR42_RS08015 0.86 +0.4 1,720,279 - RR42_RS08015 0.86 -0.8 1,720,342 + -0.6 1,720,342 + +0.1 1,720,342 + +0.3 1,720,383 - -1.7 1,720,621 + RR42_RS08020 0.45 +0.1 1,720,625 + RR42_RS08020 0.46 +0.7 1,720,770 + RR42_RS08020 0.72 -0.3 1,720,801 + RR42_RS08020 0.78 -0.1 1,720,801 + RR42_RS08020 0.78 -0.3 1,720,809 + RR42_RS08020 0.80 +0.2 1,720,809 + RR42_RS08020 0.80 +0.2 1,720,809 + RR42_RS08020 0.80 +0.3 1,721,399 + RR42_RS08025 0.50 -1.6 1,721,616 + RR42_RS08025 0.75 -0.6 1,721,971 + RR42_RS08030 0.33 +0.7 1,722,105 + RR42_RS08030 0.78 +0.4 1,722,256 + -0.8 1,722,256 + -0.1 1,722,384 + RR42_RS08035 0.12 -1.3
Or see this region's nucleotide sequence