Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07830 and RR42_RS07835 are separated by 74 nucleotides RR42_RS07835 and RR42_RS07840 are separated by 105 nucleotides
RR42_RS07830: RR42_RS07830 - N-acyl-L-amino acid amidohydrolase, at 1,677,087 to 1,678,493
_RS07830
RR42_RS07835: RR42_RS07835 - membrane protein, at 1,678,568 to 1,679,872
_RS07835
RR42_RS07840: RR42_RS07840 - acetylornithine deacetylase, at 1,679,978 to 1,681,183
_RS07840
Position (kb)
1678
1679
1680 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1677.863 kb on + strand, within RR42_RS07830 at 1677.863 kb on + strand, within RR42_RS07830 at 1677.863 kb on + strand, within RR42_RS07830 at 1677.863 kb on + strand, within RR42_RS07830 at 1677.864 kb on - strand, within RR42_RS07830 at 1677.864 kb on - strand, within RR42_RS07830 at 1677.864 kb on - strand, within RR42_RS07830 at 1677.864 kb on - strand, within RR42_RS07830 at 1677.864 kb on - strand, within RR42_RS07830 at 1677.864 kb on - strand, within RR42_RS07830 at 1677.864 kb on - strand, within RR42_RS07830 at 1678.017 kb on + strand, within RR42_RS07830 at 1678.018 kb on - strand, within RR42_RS07830 at 1678.018 kb on - strand, within RR42_RS07830 at 1678.018 kb on - strand, within RR42_RS07830 at 1678.039 kb on + strand, within RR42_RS07830 at 1678.039 kb on + strand, within RR42_RS07830 at 1678.243 kb on + strand, within RR42_RS07830 at 1678.638 kb on + strand at 1678.638 kb on + strand at 1678.638 kb on + strand at 1678.639 kb on - strand at 1678.639 kb on - strand at 1678.639 kb on - strand at 1678.639 kb on - strand at 1678.639 kb on - strand at 1678.639 kb on - strand at 1678.639 kb on - strand at 1678.806 kb on + strand, within RR42_RS07835 at 1678.807 kb on - strand, within RR42_RS07835 at 1678.947 kb on + strand, within RR42_RS07835 at 1678.947 kb on + strand, within RR42_RS07835 at 1679.058 kb on + strand, within RR42_RS07835 at 1679.059 kb on - strand, within RR42_RS07835 at 1679.070 kb on + strand, within RR42_RS07835 at 1679.070 kb on + strand, within RR42_RS07835 at 1679.070 kb on + strand, within RR42_RS07835 at 1679.070 kb on + strand, within RR42_RS07835 at 1679.070 kb on + strand, within RR42_RS07835 at 1679.070 kb on + strand, within RR42_RS07835 at 1679.071 kb on - strand, within RR42_RS07835 at 1679.071 kb on - strand, within RR42_RS07835 at 1679.071 kb on - strand, within RR42_RS07835 at 1679.071 kb on - strand, within RR42_RS07835 at 1679.071 kb on - strand, within RR42_RS07835 at 1679.071 kb on - strand, within RR42_RS07835 at 1679.082 kb on + strand, within RR42_RS07835 at 1679.082 kb on + strand, within RR42_RS07835 at 1679.224 kb on - strand, within RR42_RS07835 at 1679.224 kb on - strand, within RR42_RS07835 at 1679.224 kb on - strand, within RR42_RS07835 at 1679.224 kb on - strand, within RR42_RS07835 at 1679.226 kb on + strand, within RR42_RS07835 at 1679.227 kb on - strand, within RR42_RS07835 at 1679.227 kb on - strand, within RR42_RS07835 at 1679.512 kb on - strand, within RR42_RS07835 at 1679.601 kb on + strand, within RR42_RS07835 at 1679.737 kb on - strand, within RR42_RS07835 at 1680.309 kb on + strand, within RR42_RS07840 at 1680.309 kb on + strand, within RR42_RS07840 at 1680.310 kb on - strand, within RR42_RS07840 at 1680.469 kb on - strand, within RR42_RS07840 at 1680.705 kb on + strand, within RR42_RS07840 at 1680.706 kb on - strand, within RR42_RS07840 at 1680.706 kb on - strand, within RR42_RS07840 at 1680.751 kb on - strand, within RR42_RS07840 at 1680.751 kb on - strand, within RR42_RS07840 at 1680.751 kb on - strand, within RR42_RS07840
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 1,677,863 + RR42_RS07830 0.55 +0.5 1,677,863 + RR42_RS07830 0.55 -0.3 1,677,863 + RR42_RS07830 0.55 +0.2 1,677,863 + RR42_RS07830 0.55 -1.0 1,677,864 - RR42_RS07830 0.55 -0.2 1,677,864 - RR42_RS07830 0.55 +0.0 1,677,864 - RR42_RS07830 0.55 -0.9 1,677,864 - RR42_RS07830 0.55 -0.3 1,677,864 - RR42_RS07830 0.55 +1.2 1,677,864 - RR42_RS07830 0.55 +1.2 1,677,864 - RR42_RS07830 0.55 +0.4 1,678,017 + RR42_RS07830 0.66 -1.1 1,678,018 - RR42_RS07830 0.66 -0.1 1,678,018 - RR42_RS07830 0.66 -0.9 1,678,018 - RR42_RS07830 0.66 -0.1 1,678,039 + RR42_RS07830 0.68 +0.2 1,678,039 + RR42_RS07830 0.68 +0.6 1,678,243 + RR42_RS07830 0.82 +0.2 1,678,638 + -0.2 1,678,638 + -1.0 1,678,638 + -0.0 1,678,639 - -0.1 1,678,639 - +0.5 1,678,639 - -1.2 1,678,639 - -0.0 1,678,639 - +0.6 1,678,639 - -0.2 1,678,639 - +1.4 1,678,806 + RR42_RS07835 0.18 +0.3 1,678,807 - RR42_RS07835 0.18 +0.6 1,678,947 + RR42_RS07835 0.29 +0.6 1,678,947 + RR42_RS07835 0.29 -0.1 1,679,058 + RR42_RS07835 0.38 +0.4 1,679,059 - RR42_RS07835 0.38 +0.1 1,679,070 + RR42_RS07835 0.38 -0.3 1,679,070 + RR42_RS07835 0.38 +0.4 1,679,070 + RR42_RS07835 0.38 +0.2 1,679,070 + RR42_RS07835 0.38 +0.8 1,679,070 + RR42_RS07835 0.38 +0.1 1,679,070 + RR42_RS07835 0.38 -0.6 1,679,071 - RR42_RS07835 0.39 +0.0 1,679,071 - RR42_RS07835 0.39 -0.0 1,679,071 - RR42_RS07835 0.39 +1.1 1,679,071 - RR42_RS07835 0.39 +0.7 1,679,071 - RR42_RS07835 0.39 +0.2 1,679,071 - RR42_RS07835 0.39 +0.2 1,679,082 + RR42_RS07835 0.39 +0.0 1,679,082 + RR42_RS07835 0.39 -0.9 1,679,224 - RR42_RS07835 0.50 -0.1 1,679,224 - RR42_RS07835 0.50 +0.5 1,679,224 - RR42_RS07835 0.50 -0.5 1,679,224 - RR42_RS07835 0.50 -1.0 1,679,226 + RR42_RS07835 0.50 -0.3 1,679,227 - RR42_RS07835 0.50 +0.2 1,679,227 - RR42_RS07835 0.50 -2.8 1,679,512 - RR42_RS07835 0.72 -0.1 1,679,601 + RR42_RS07835 0.79 -0.1 1,679,737 - RR42_RS07835 0.90 -0.5 1,680,309 + RR42_RS07840 0.27 +0.2 1,680,309 + RR42_RS07840 0.27 -0.6 1,680,310 - RR42_RS07840 0.28 +0.0 1,680,469 - RR42_RS07840 0.41 +0.7 1,680,705 + RR42_RS07840 0.60 +0.3 1,680,706 - RR42_RS07840 0.60 +1.3 1,680,706 - RR42_RS07840 0.60 -0.3 1,680,751 - RR42_RS07840 0.64 +0.1 1,680,751 - RR42_RS07840 0.64 +0.7 1,680,751 - RR42_RS07840 0.64 -0.4
Or see this region's nucleotide sequence