Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05300 and RR42_RS05305 are separated by 282 nucleotides RR42_RS05305 and RR42_RS05310 are separated by 95 nucleotides RR42_RS05310 and RR42_RS05315 are separated by 218 nucleotides
RR42_RS05300: RR42_RS05300 - TonB-dependent receptor, at 1,170,036 to 1,172,360
_RS05300
RR42_RS05305: RR42_RS05305 - NADP oxidoreductase, at 1,172,643 to 1,173,416
_RS05305
RR42_RS05310: RR42_RS05310 - hypothetical protein, at 1,173,512 to 1,173,940
_RS05310
RR42_RS05315: RR42_RS05315 - fatty acid desaturase, at 1,174,159 to 1,175,259
_RS05315
Position (kb)
1172
1173
1174 Strain fitness (log2 ratio)
-2
-1
0
1 at 1171.767 kb on + strand, within RR42_RS05300 at 1171.768 kb on - strand at 1171.846 kb on + strand, within RR42_RS05300 at 1171.846 kb on + strand, within RR42_RS05300 at 1171.846 kb on + strand, within RR42_RS05300 at 1171.847 kb on - strand, within RR42_RS05300 at 1171.958 kb on - strand, within RR42_RS05300 at 1172.011 kb on + strand, within RR42_RS05300 at 1172.011 kb on + strand, within RR42_RS05300 at 1172.043 kb on + strand, within RR42_RS05300 at 1172.044 kb on - strand, within RR42_RS05300 at 1172.458 kb on + strand at 1172.458 kb on + strand at 1172.458 kb on + strand at 1172.462 kb on + strand at 1172.462 kb on + strand at 1172.462 kb on + strand at 1172.462 kb on + strand at 1172.463 kb on - strand at 1172.463 kb on - strand at 1172.463 kb on - strand at 1172.463 kb on - strand at 1172.463 kb on - strand at 1172.467 kb on + strand at 1172.468 kb on - strand at 1172.967 kb on + strand, within RR42_RS05305 at 1172.967 kb on + strand, within RR42_RS05305 at 1172.968 kb on - strand, within RR42_RS05305 at 1172.968 kb on - strand, within RR42_RS05305 at 1172.997 kb on + strand, within RR42_RS05305 at 1173.115 kb on - strand, within RR42_RS05305 at 1173.588 kb on + strand, within RR42_RS05310 at 1173.589 kb on - strand, within RR42_RS05310 at 1173.732 kb on + strand, within RR42_RS05310 at 1173.822 kb on - strand, within RR42_RS05310 at 1174.029 kb on + strand at 1174.030 kb on - strand at 1174.086 kb on + strand at 1174.094 kb on + strand at 1174.095 kb on - strand at 1174.095 kb on - strand at 1174.232 kb on + strand at 1174.233 kb on - strand at 1174.233 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 1,171,767 + RR42_RS05300 0.74 -0.6 1,171,768 - -0.6 1,171,846 + RR42_RS05300 0.78 +1.0 1,171,846 + RR42_RS05300 0.78 +0.2 1,171,846 + RR42_RS05300 0.78 -1.0 1,171,847 - RR42_RS05300 0.78 +1.0 1,171,958 - RR42_RS05300 0.83 -0.0 1,172,011 + RR42_RS05300 0.85 +1.0 1,172,011 + RR42_RS05300 0.85 -1.7 1,172,043 + RR42_RS05300 0.86 +0.4 1,172,044 - RR42_RS05300 0.86 -0.1 1,172,458 + -2.6 1,172,458 + -0.5 1,172,458 + +0.6 1,172,462 + -0.9 1,172,462 + -2.5 1,172,462 + -0.2 1,172,462 + -0.2 1,172,463 - -0.2 1,172,463 - +0.3 1,172,463 - -0.4 1,172,463 - -0.6 1,172,463 - -0.7 1,172,467 + -0.5 1,172,468 - -0.8 1,172,967 + RR42_RS05305 0.42 -0.6 1,172,967 + RR42_RS05305 0.42 -1.3 1,172,968 - RR42_RS05305 0.42 +0.2 1,172,968 - RR42_RS05305 0.42 -1.2 1,172,997 + RR42_RS05305 0.46 -0.1 1,173,115 - RR42_RS05305 0.61 -1.2 1,173,588 + RR42_RS05310 0.18 -0.5 1,173,589 - RR42_RS05310 0.18 +0.7 1,173,732 + RR42_RS05310 0.51 -1.0 1,173,822 - RR42_RS05310 0.72 -0.0 1,174,029 + -0.3 1,174,030 - -0.9 1,174,086 + +1.0 1,174,094 + +0.1 1,174,095 - -0.3 1,174,095 - +0.7 1,174,232 + -1.8 1,174,233 - +0.3 1,174,233 - +0.3
Or see this region's nucleotide sequence