Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04270 and RR42_RS04275 are separated by 116 nucleotides RR42_RS04275 and RR42_RS04280 overlap by 8 nucleotides
RR42_RS04270: RR42_RS04270 - MFS transporter, at 949,957 to 951,261
_RS04270
RR42_RS04275: RR42_RS04275 - hypothetical protein, at 951,378 to 951,620
_RS04275
RR42_RS04280: RR42_RS04280 - aldehyde dehydrogenase, at 951,613 to 953,049
_RS04280
Position (kb) 951
952 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 950.379 kb on - strand, within RR42_RS04270 at 950.465 kb on + strand, within RR42_RS04270 at 950.466 kb on - strand, within RR42_RS04270 at 950.489 kb on + strand, within RR42_RS04270 at 950.575 kb on - strand, within RR42_RS04270 at 950.841 kb on - strand, within RR42_RS04270 at 950.910 kb on - strand, within RR42_RS04270 at 950.910 kb on - strand, within RR42_RS04270 at 950.910 kb on - strand, within RR42_RS04270 at 950.910 kb on - strand, within RR42_RS04270 at 950.910 kb on - strand, within RR42_RS04270 at 951.042 kb on - strand, within RR42_RS04270 at 951.074 kb on + strand, within RR42_RS04270 at 951.158 kb on + strand at 951.159 kb on - strand at 951.203 kb on + strand at 951.203 kb on + strand at 951.204 kb on - strand at 951.244 kb on + strand at 951.244 kb on + strand at 951.245 kb on - strand at 951.288 kb on + strand at 951.288 kb on + strand at 951.289 kb on - strand at 951.289 kb on - strand at 951.455 kb on - strand, within RR42_RS04275 at 951.611 kb on + strand at 951.611 kb on + strand at 951.612 kb on - strand at 951.614 kb on + strand at 951.615 kb on - strand at 951.615 kb on - strand at 951.687 kb on - strand at 951.715 kb on + strand at 951.721 kb on + strand at 951.740 kb on + strand at 951.740 kb on + strand at 951.740 kb on + strand at 951.969 kb on - strand, within RR42_RS04280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 950,379 - RR42_RS04270 0.32 +0.4 950,465 + RR42_RS04270 0.39 -0.5 950,466 - RR42_RS04270 0.39 -3.2 950,489 + RR42_RS04270 0.41 +0.0 950,575 - RR42_RS04270 0.47 +0.8 950,841 - RR42_RS04270 0.68 -1.1 950,910 - RR42_RS04270 0.73 -1.3 950,910 - RR42_RS04270 0.73 -1.5 950,910 - RR42_RS04270 0.73 +0.3 950,910 - RR42_RS04270 0.73 +0.0 950,910 - RR42_RS04270 0.73 -0.8 951,042 - RR42_RS04270 0.83 -0.6 951,074 + RR42_RS04270 0.86 -0.5 951,158 + -0.3 951,159 - -0.5 951,203 + +0.1 951,203 + +0.5 951,204 - -0.2 951,244 + -0.0 951,244 + -0.4 951,245 - +0.0 951,288 + -0.7 951,288 + -0.0 951,289 - -1.5 951,289 - +0.6 951,455 - RR42_RS04275 0.32 +0.0 951,611 + +0.5 951,611 + -0.2 951,612 - -0.5 951,614 + +0.1 951,615 - +0.4 951,615 - -1.3 951,687 - +0.2 951,715 + -1.3 951,721 + -1.5 951,740 + +0.8 951,740 + -1.2 951,740 + -0.1 951,969 - RR42_RS04280 0.25 +0.3
Or see this region's nucleotide sequence