Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04250 and RR42_RS04255 are separated by 66 nucleotides RR42_RS04255 and RR42_RS04260 are separated by 87 nucleotides RR42_RS04260 and RR42_RS04265 are separated by 85 nucleotides
RR42_RS04250: RR42_RS04250 - chemotaxis protein CheY, at 940,069 to 940,437
_RS04250
RR42_RS04255: RR42_RS04255 - chemotaxis protein CheW, at 940,504 to 941,091
_RS04255
RR42_RS04260: RR42_RS04260 - chemotaxis protein, at 941,179 to 943,347
_RS04260
RR42_RS04265: RR42_RS04265 - chemotaxis protein CheY, at 943,433 to 949,480
_RS04265
Position (kb)
941
942
943
944 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 940.256 kb on - strand, within RR42_RS04250 at 940.376 kb on - strand, within RR42_RS04250 at 940.376 kb on - strand, within RR42_RS04250 at 940.376 kb on - strand, within RR42_RS04250 at 940.376 kb on - strand, within RR42_RS04250 at 940.555 kb on + strand at 940.555 kb on + strand at 940.723 kb on + strand, within RR42_RS04255 at 940.723 kb on + strand, within RR42_RS04255 at 941.178 kb on + strand at 941.179 kb on - strand at 941.179 kb on - strand at 941.387 kb on - strand at 941.387 kb on - strand at 941.926 kb on - strand, within RR42_RS04260 at 941.926 kb on - strand, within RR42_RS04260 at 941.926 kb on - strand, within RR42_RS04260 at 941.926 kb on - strand, within RR42_RS04260 at 941.926 kb on - strand, within RR42_RS04260 at 942.265 kb on + strand, within RR42_RS04260 at 942.266 kb on - strand at 942.266 kb on - strand, within RR42_RS04260 at 942.478 kb on + strand, within RR42_RS04260 at 942.478 kb on + strand, within RR42_RS04260 at 942.479 kb on - strand, within RR42_RS04260 at 942.479 kb on - strand, within RR42_RS04260 at 942.479 kb on - strand, within RR42_RS04260 at 942.479 kb on - strand, within RR42_RS04260 at 943.200 kb on + strand at 943.218 kb on + strand at 943.219 kb on - strand at 943.317 kb on + strand at 943.756 kb on - strand at 943.782 kb on - strand at 943.889 kb on + strand at 943.890 kb on - strand at 944.153 kb on + strand, within RR42_RS04265 at 944.153 kb on + strand, within RR42_RS04265 at 944.153 kb on + strand, within RR42_RS04265 at 944.153 kb on + strand, within RR42_RS04265 at 944.185 kb on - strand, within RR42_RS04265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 940,256 - RR42_RS04250 0.51 -0.2 940,376 - RR42_RS04250 0.83 +0.6 940,376 - RR42_RS04250 0.83 +1.0 940,376 - RR42_RS04250 0.83 -0.8 940,376 - RR42_RS04250 0.83 +1.4 940,555 + +0.7 940,555 + -0.3 940,723 + RR42_RS04255 0.37 -1.2 940,723 + RR42_RS04255 0.37 -2.9 941,178 + +0.7 941,179 - +0.2 941,179 - -0.3 941,387 - -0.4 941,387 - +0.0 941,926 - RR42_RS04260 0.34 -1.1 941,926 - RR42_RS04260 0.34 +0.4 941,926 - RR42_RS04260 0.34 -0.2 941,926 - RR42_RS04260 0.34 -0.3 941,926 - RR42_RS04260 0.34 -0.1 942,265 + RR42_RS04260 0.50 +1.2 942,266 - +0.0 942,266 - RR42_RS04260 0.50 +0.3 942,478 + RR42_RS04260 0.60 -0.4 942,478 + RR42_RS04260 0.60 +1.0 942,479 - RR42_RS04260 0.60 +1.0 942,479 - RR42_RS04260 0.60 +0.4 942,479 - RR42_RS04260 0.60 +0.0 942,479 - RR42_RS04260 0.60 +0.7 943,200 + -1.4 943,218 + -0.1 943,219 - -0.3 943,317 + +0.8 943,756 - -0.8 943,782 - -0.1 943,889 + +0.5 943,890 - -0.9 944,153 + RR42_RS04265 0.12 +0.1 944,153 + RR42_RS04265 0.12 +1.6 944,153 + RR42_RS04265 0.12 +0.4 944,153 + RR42_RS04265 0.12 +1.0 944,185 - RR42_RS04265 0.12 -0.7
Or see this region's nucleotide sequence