Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03045 and RR42_RS03050 are separated by 106 nucleotides RR42_RS03050 and RR42_RS03055 are separated by 300 nucleotides RR42_RS03055 and RR42_RS03060 are separated by 204 nucleotides
RR42_RS03045: RR42_RS03045 - dioxygenase, at 674,200 to 675,021
_RS03045
RR42_RS03050: RR42_RS03050 - energy transducer TonB, at 675,128 to 675,868
_RS03050
RR42_RS03055: RR42_RS03055 - tRNA-Met, at 676,169 to 676,245
_RS03055
RR42_RS03060: RR42_RS03060 - MFS transporter, at 676,450 to 678,150
_RS03060
Position (kb)
675
676 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 674.216 kb on - strand at 674.407 kb on + strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.473 kb on + strand, within RR42_RS03045 at 674.474 kb on - strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.533 kb on - strand, within RR42_RS03045 at 674.552 kb on - strand, within RR42_RS03045 at 674.552 kb on - strand, within RR42_RS03045 at 674.819 kb on - strand, within RR42_RS03045 at 674.969 kb on + strand at 675.762 kb on + strand, within RR42_RS03050 at 675.762 kb on + strand, within RR42_RS03050 at 675.766 kb on - strand, within RR42_RS03050 at 675.766 kb on - strand, within RR42_RS03050 at 675.766 kb on - strand, within RR42_RS03050 at 675.846 kb on + strand at 675.847 kb on - strand at 675.847 kb on - strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.901 kb on - strand at 675.901 kb on - strand at 675.901 kb on - strand at 675.901 kb on - strand at 676.004 kb on + strand at 676.004 kb on + strand at 676.004 kb on + strand at 676.004 kb on + strand at 676.005 kb on - strand at 676.167 kb on + strand at 676.168 kb on - strand at 676.463 kb on + strand at 676.463 kb on + strand at 676.463 kb on + strand at 676.464 kb on - strand at 676.464 kb on - strand at 676.550 kb on + strand at 676.551 kb on - strand at 676.553 kb on + strand at 676.553 kb on + strand at 676.631 kb on + strand, within RR42_RS03060 at 676.632 kb on - strand, within RR42_RS03060 at 676.632 kb on - strand, within RR42_RS03060 at 676.653 kb on - strand, within RR42_RS03060 at 676.653 kb on - strand, within RR42_RS03060 at 676.703 kb on + strand, within RR42_RS03060 at 676.704 kb on - strand, within RR42_RS03060 at 676.718 kb on + strand, within RR42_RS03060 at 676.719 kb on - strand, within RR42_RS03060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 674,216 - -1.5 674,407 + RR42_RS03045 0.25 +0.9 674,408 - RR42_RS03045 0.25 +1.7 674,408 - RR42_RS03045 0.25 +1.3 674,408 - RR42_RS03045 0.25 -0.7 674,473 + RR42_RS03045 0.33 -0.4 674,474 - RR42_RS03045 0.33 +0.2 674,530 + RR42_RS03045 0.40 -0.0 674,530 + RR42_RS03045 0.40 +0.2 674,530 + RR42_RS03045 0.40 -0.9 674,530 + RR42_RS03045 0.40 -1.0 674,530 + RR42_RS03045 0.40 -0.1 674,531 - RR42_RS03045 0.40 -0.8 674,531 - RR42_RS03045 0.40 +0.1 674,531 - RR42_RS03045 0.40 -0.5 674,531 - RR42_RS03045 0.40 +0.4 674,531 - RR42_RS03045 0.40 -0.4 674,531 - RR42_RS03045 0.40 -0.8 674,531 - RR42_RS03045 0.40 +0.2 674,531 - RR42_RS03045 0.40 -0.2 674,532 + RR42_RS03045 0.40 -0.3 674,532 + RR42_RS03045 0.40 +0.0 674,532 + RR42_RS03045 0.40 +0.3 674,532 + RR42_RS03045 0.40 -0.6 674,532 + RR42_RS03045 0.40 +0.5 674,533 - RR42_RS03045 0.41 -0.3 674,552 - RR42_RS03045 0.43 -0.9 674,552 - RR42_RS03045 0.43 -0.2 674,819 - RR42_RS03045 0.75 -1.3 674,969 + +1.0 675,762 + RR42_RS03050 0.86 -0.1 675,762 + RR42_RS03050 0.86 -1.2 675,766 - RR42_RS03050 0.86 -2.4 675,766 - RR42_RS03050 0.86 +1.2 675,766 - RR42_RS03050 0.86 -0.9 675,846 + -0.2 675,847 - -1.8 675,847 - -0.4 675,900 + -0.3 675,900 + -1.8 675,900 + +0.1 675,900 + +1.8 675,900 + -1.0 675,901 - -0.6 675,901 - +1.9 675,901 - +0.9 675,901 - +0.7 676,004 + -0.3 676,004 + +0.0 676,004 + -0.4 676,004 + +0.8 676,005 - -0.7 676,167 + -0.8 676,168 - -2.6 676,463 + +0.6 676,463 + -0.4 676,463 + -0.6 676,464 - +0.1 676,464 - +0.1 676,550 + -0.7 676,551 - +0.3 676,553 + +0.9 676,553 + -0.4 676,631 + RR42_RS03060 0.11 +0.4 676,632 - RR42_RS03060 0.11 +0.7 676,632 - RR42_RS03060 0.11 +1.1 676,653 - RR42_RS03060 0.12 +1.4 676,653 - RR42_RS03060 0.12 -0.4 676,703 + RR42_RS03060 0.15 -0.1 676,704 - RR42_RS03060 0.15 -0.1 676,718 + RR42_RS03060 0.16 -0.0 676,719 - RR42_RS03060 0.16 +0.6
Or see this region's nucleotide sequence