Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02910 and RR42_RS02915 are separated by 220 nucleotides RR42_RS02915 and RR42_RS02920 are separated by 51 nucleotides RR42_RS02920 and RR42_RS02925 are separated by 141 nucleotides
RR42_RS02910: RR42_RS02910 - 2-aminoadipate aminotransferase, at 648,964 to 650,214
_RS02910
RR42_RS02915: RR42_RS02915 - hypothetical protein, at 650,435 to 651,466
_RS02915
RR42_RS02920: RR42_RS02920 - hypothetical protein, at 651,518 to 651,892
_RS02920
RR42_RS02925: RR42_RS02925 - membrane protein, at 652,034 to 652,945
_RS02925
Position (kb)
650
651
652 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 649.690 kb on + strand, within RR42_RS02910 at 649.888 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.050 kb on + strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.051 kb on - strand, within RR42_RS02910 at 650.248 kb on - strand at 650.364 kb on + strand at 650.364 kb on + strand at 650.365 kb on - strand at 650.365 kb on - strand at 650.368 kb on + strand at 650.368 kb on + strand at 650.369 kb on - strand at 650.369 kb on - strand at 650.369 kb on - strand at 650.403 kb on + strand at 650.426 kb on + strand at 650.682 kb on - strand, within RR42_RS02915 at 650.682 kb on - strand, within RR42_RS02915 at 650.682 kb on - strand, within RR42_RS02915 at 650.750 kb on - strand, within RR42_RS02915 at 650.788 kb on + strand, within RR42_RS02915 at 650.788 kb on + strand, within RR42_RS02915 at 650.788 kb on + strand, within RR42_RS02915 at 650.966 kb on + strand, within RR42_RS02915 at 650.966 kb on + strand, within RR42_RS02915 at 650.967 kb on - strand, within RR42_RS02915 at 650.976 kb on + strand, within RR42_RS02915 at 651.056 kb on + strand, within RR42_RS02915 at 651.223 kb on + strand, within RR42_RS02915 at 651.266 kb on + strand, within RR42_RS02915 at 651.377 kb on + strand at 651.378 kb on - strand at 651.465 kb on - strand at 651.649 kb on - strand, within RR42_RS02920 at 651.649 kb on - strand, within RR42_RS02920 at 651.652 kb on - strand, within RR42_RS02920 at 651.663 kb on + strand, within RR42_RS02920 at 651.663 kb on + strand, within RR42_RS02920 at 651.684 kb on - strand, within RR42_RS02920 at 651.705 kb on + strand, within RR42_RS02920 at 651.705 kb on + strand, within RR42_RS02920 at 651.706 kb on - strand, within RR42_RS02920 at 651.706 kb on - strand, within RR42_RS02920 at 651.706 kb on - strand, within RR42_RS02920 at 651.723 kb on + strand, within RR42_RS02920 at 651.723 kb on + strand, within RR42_RS02920 at 651.741 kb on + strand, within RR42_RS02920 at 651.925 kb on - strand at 651.925 kb on - strand at 651.947 kb on + strand at 651.947 kb on + strand at 651.947 kb on + strand at 651.948 kb on - strand at 651.961 kb on + strand at 651.961 kb on + strand at 651.961 kb on + strand at 651.962 kb on - strand at 651.962 kb on - strand at 651.962 kb on - strand at 651.980 kb on - strand at 652.249 kb on + strand, within RR42_RS02925 at 652.249 kb on + strand, within RR42_RS02925 at 652.250 kb on - strand, within RR42_RS02925
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 649,690 + RR42_RS02910 0.58 -2.1 649,888 + RR42_RS02910 0.74 -1.3 650,050 + RR42_RS02910 0.87 +0.1 650,050 + RR42_RS02910 0.87 -0.6 650,050 + RR42_RS02910 0.87 +0.2 650,050 + RR42_RS02910 0.87 +0.4 650,050 + RR42_RS02910 0.87 -0.3 650,050 + RR42_RS02910 0.87 -0.1 650,050 + RR42_RS02910 0.87 -0.1 650,051 - RR42_RS02910 0.87 +0.5 650,051 - RR42_RS02910 0.87 -0.9 650,051 - RR42_RS02910 0.87 -0.5 650,051 - RR42_RS02910 0.87 -0.1 650,051 - RR42_RS02910 0.87 -0.4 650,051 - RR42_RS02910 0.87 -0.1 650,248 - +0.0 650,364 + -0.4 650,364 + -1.8 650,365 - +0.7 650,365 - +0.5 650,368 + +0.1 650,368 + -0.0 650,369 - -0.2 650,369 - -2.4 650,369 - +1.0 650,403 + -1.6 650,426 + +0.0 650,682 - RR42_RS02915 0.24 -0.5 650,682 - RR42_RS02915 0.24 +0.1 650,682 - RR42_RS02915 0.24 +0.6 650,750 - RR42_RS02915 0.31 -0.2 650,788 + RR42_RS02915 0.34 -0.7 650,788 + RR42_RS02915 0.34 -0.8 650,788 + RR42_RS02915 0.34 +0.8 650,966 + RR42_RS02915 0.51 -0.2 650,966 + RR42_RS02915 0.51 -1.4 650,967 - RR42_RS02915 0.52 -0.6 650,976 + RR42_RS02915 0.52 +0.2 651,056 + RR42_RS02915 0.60 -0.1 651,223 + RR42_RS02915 0.76 +0.2 651,266 + RR42_RS02915 0.81 -1.6 651,377 + +0.1 651,378 - +0.4 651,465 - -1.4 651,649 - RR42_RS02920 0.35 +0.6 651,649 - RR42_RS02920 0.35 +1.7 651,652 - RR42_RS02920 0.36 +0.4 651,663 + RR42_RS02920 0.39 +0.3 651,663 + RR42_RS02920 0.39 -0.6 651,684 - RR42_RS02920 0.44 +2.2 651,705 + RR42_RS02920 0.50 +0.1 651,705 + RR42_RS02920 0.50 +0.2 651,706 - RR42_RS02920 0.50 -1.1 651,706 - RR42_RS02920 0.50 -0.1 651,706 - RR42_RS02920 0.50 +0.7 651,723 + RR42_RS02920 0.55 -1.4 651,723 + RR42_RS02920 0.55 -1.7 651,741 + RR42_RS02920 0.59 -2.1 651,925 - +1.9 651,925 - -0.7 651,947 + +0.9 651,947 + -0.4 651,947 + -0.8 651,948 - +0.4 651,961 + -0.2 651,961 + -1.6 651,961 + -0.4 651,962 - +0.2 651,962 - -1.1 651,962 - -0.1 651,980 - +1.0 652,249 + RR42_RS02925 0.24 +1.0 652,249 + RR42_RS02925 0.24 -0.8 652,250 - RR42_RS02925 0.24 +0.3
Or see this region's nucleotide sequence