Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01885 and RR42_RS01890 are separated by 34 nucleotides RR42_RS01890 and RR42_RS01895 are separated by 135 nucleotides RR42_RS01895 and RR42_RS01900 are separated by 175 nucleotides RR42_RS01900 and RR42_RS01905 are separated by 78 nucleotides
RR42_RS01885: RR42_RS01885 - cytochrome C oxidase assembly protein, at 425,766 to 426,380
_RS01885
RR42_RS01890: RR42_RS01890 - membrane protein, at 426,415 to 426,627
_RS01890
RR42_RS01895: RR42_RS01895 - MFS transporter, at 426,763 to 427,623
_RS01895
RR42_RS01900: RR42_RS01900 - membrane protein, at 427,799 to 428,008
_RS01900
RR42_RS01905: RR42_RS01905 - cytochrome C oxidase subunit II, at 428,087 to 428,848
_RS01905
Position (kb) 426
427
428 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 425.886 kb on + strand, within RR42_RS01885 at 425.941 kb on - strand, within RR42_RS01885 at 426.139 kb on - strand, within RR42_RS01885 at 426.282 kb on - strand, within RR42_RS01885 at 426.282 kb on - strand, within RR42_RS01885 at 426.291 kb on + strand, within RR42_RS01885 at 426.292 kb on - strand, within RR42_RS01885 at 426.294 kb on - strand, within RR42_RS01885 at 426.662 kb on + strand at 426.663 kb on - strand at 426.666 kb on + strand at 426.666 kb on + strand at 426.791 kb on - strand at 426.791 kb on - strand at 426.793 kb on + strand at 426.794 kb on - strand at 426.831 kb on + strand at 426.898 kb on + strand, within RR42_RS01895 at 426.898 kb on + strand, within RR42_RS01895 at 426.898 kb on + strand, within RR42_RS01895 at 426.986 kb on - strand, within RR42_RS01895 at 427.081 kb on + strand, within RR42_RS01895 at 427.082 kb on - strand, within RR42_RS01895 at 427.154 kb on + strand, within RR42_RS01895 at 427.299 kb on + strand, within RR42_RS01895 at 427.366 kb on + strand, within RR42_RS01895 at 427.366 kb on + strand, within RR42_RS01895 at 427.420 kb on + strand, within RR42_RS01895 at 427.421 kb on - strand, within RR42_RS01895 at 427.423 kb on - strand, within RR42_RS01895 at 427.564 kb on + strand at 427.601 kb on - strand at 427.621 kb on + strand at 427.622 kb on - strand at 427.622 kb on - strand at 427.723 kb on + strand at 427.724 kb on - strand at 427.724 kb on - strand at 427.724 kb on - strand at 427.724 kb on - strand at 427.724 kb on - strand at 427.830 kb on - strand, within RR42_RS01900 at 427.830 kb on - strand, within RR42_RS01900 at 427.830 kb on - strand, within RR42_RS01900 at 427.966 kb on + strand, within RR42_RS01900 at 427.966 kb on + strand, within RR42_RS01900 at 428.036 kb on - strand at 428.041 kb on + strand at 428.041 kb on + strand at 428.042 kb on - strand at 428.042 kb on - strand at 428.338 kb on + strand, within RR42_RS01905 at 428.339 kb on - strand, within RR42_RS01905 at 428.498 kb on - strand, within RR42_RS01905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 425,886 + RR42_RS01885 0.20 -1.0 425,941 - RR42_RS01885 0.28 -1.3 426,139 - RR42_RS01885 0.61 -1.7 426,282 - RR42_RS01885 0.84 -2.2 426,282 - RR42_RS01885 0.84 -1.5 426,291 + RR42_RS01885 0.85 -0.4 426,292 - RR42_RS01885 0.86 -1.6 426,294 - RR42_RS01885 0.86 -2.3 426,662 + +2.0 426,663 - -0.2 426,666 + -0.1 426,666 + -1.1 426,791 - -0.2 426,791 - +0.7 426,793 + -1.1 426,794 - -0.9 426,831 + -2.6 426,898 + RR42_RS01895 0.16 -0.6 426,898 + RR42_RS01895 0.16 -1.0 426,898 + RR42_RS01895 0.16 -0.2 426,986 - RR42_RS01895 0.26 -1.3 427,081 + RR42_RS01895 0.37 -0.7 427,082 - RR42_RS01895 0.37 -0.4 427,154 + RR42_RS01895 0.45 -1.1 427,299 + RR42_RS01895 0.62 -2.8 427,366 + RR42_RS01895 0.70 -1.3 427,366 + RR42_RS01895 0.70 -3.9 427,420 + RR42_RS01895 0.76 -1.1 427,421 - RR42_RS01895 0.76 +1.3 427,423 - RR42_RS01895 0.77 -0.6 427,564 + -1.0 427,601 - +0.0 427,621 + -0.4 427,622 - +1.3 427,622 - -0.2 427,723 + +0.6 427,724 - -0.0 427,724 - -0.0 427,724 - +1.0 427,724 - +0.3 427,724 - +0.4 427,830 - RR42_RS01900 0.15 -0.1 427,830 - RR42_RS01900 0.15 -0.3 427,830 - RR42_RS01900 0.15 -0.2 427,966 + RR42_RS01900 0.80 +0.5 427,966 + RR42_RS01900 0.80 -0.2 428,036 - +0.2 428,041 + -0.6 428,041 + -0.4 428,042 - -0.7 428,042 - -0.1 428,338 + RR42_RS01905 0.33 +0.1 428,339 - RR42_RS01905 0.33 +0.1 428,498 - RR42_RS01905 0.54 -1.7
Or see this region's nucleotide sequence