Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01570 and RR42_RS01575 are separated by 355 nucleotides RR42_RS01575 and RR42_RS01580 are separated by 46 nucleotides
RR42_RS01570: RR42_RS01570 - GntR family transcriptional regulator, at 353,603 to 355,120
_RS01570
RR42_RS01575: RR42_RS01575 - omega amino acid--pyruvate aminotransferase, at 355,476 to 356,807
_RS01575
RR42_RS01580: RR42_RS01580 - methylmalonate-semialdehyde dehydrogenase, at 356,854 to 358,371
_RS01580
Position (kb) 355
356
357 Strain fitness (log2 ratio) -2
-1
0
1
2 at 354.631 kb on + strand, within RR42_RS01570 at 354.942 kb on + strand, within RR42_RS01570 at 355.132 kb on - strand at 355.132 kb on - strand at 355.309 kb on + strand at 355.310 kb on - strand at 355.333 kb on - strand at 355.359 kb on + strand at 355.359 kb on + strand at 355.360 kb on - strand at 355.361 kb on + strand at 355.361 kb on + strand at 355.362 kb on - strand at 355.369 kb on - strand at 355.387 kb on - strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.475 kb on + strand at 355.476 kb on - strand at 355.476 kb on - strand at 355.476 kb on - strand at 355.476 kb on - strand at 355.476 kb on - strand at 355.546 kb on - strand at 355.587 kb on + strand at 355.587 kb on + strand at 355.587 kb on + strand at 355.588 kb on - strand at 355.588 kb on - strand at 355.590 kb on + strand at 355.590 kb on + strand at 355.591 kb on - strand at 355.857 kb on + strand, within RR42_RS01575 at 355.857 kb on + strand, within RR42_RS01575 at 355.857 kb on + strand, within RR42_RS01575 at 355.858 kb on - strand, within RR42_RS01575 at 356.024 kb on + strand, within RR42_RS01575 at 356.024 kb on + strand, within RR42_RS01575 at 356.025 kb on - strand, within RR42_RS01575 at 356.040 kb on + strand, within RR42_RS01575 at 356.185 kb on - strand, within RR42_RS01575 at 356.437 kb on - strand, within RR42_RS01575 at 356.437 kb on - strand, within RR42_RS01575 at 356.437 kb on - strand, within RR42_RS01575 at 356.442 kb on + strand, within RR42_RS01575 at 356.443 kb on - strand, within RR42_RS01575 at 356.443 kb on - strand, within RR42_RS01575 at 356.443 kb on - strand, within RR42_RS01575 at 356.488 kb on - strand, within RR42_RS01575 at 356.489 kb on + strand at 356.489 kb on + strand, within RR42_RS01575 at 356.490 kb on - strand, within RR42_RS01575 at 356.490 kb on - strand, within RR42_RS01575 at 356.490 kb on - strand, within RR42_RS01575 at 356.493 kb on + strand, within RR42_RS01575 at 356.493 kb on + strand, within RR42_RS01575 at 356.493 kb on + strand, within RR42_RS01575 at 356.494 kb on - strand, within RR42_RS01575 at 356.494 kb on - strand, within RR42_RS01575 at 356.494 kb on - strand, within RR42_RS01575 at 356.494 kb on - strand, within RR42_RS01575 at 356.658 kb on + strand, within RR42_RS01575 at 356.897 kb on - strand at 357.381 kb on + strand, within RR42_RS01580 at 357.381 kb on + strand, within RR42_RS01580 at 357.381 kb on + strand, within RR42_RS01580 at 357.382 kb on - strand, within RR42_RS01580 at 357.571 kb on + strand, within RR42_RS01580 at 357.572 kb on - strand, within RR42_RS01580 at 357.578 kb on - strand, within RR42_RS01580 at 357.697 kb on + strand, within RR42_RS01580 at 357.698 kb on - strand, within RR42_RS01580
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 354,631 + RR42_RS01570 0.68 +0.5 354,942 + RR42_RS01570 0.88 -0.4 355,132 - -0.2 355,132 - +0.6 355,309 + -0.5 355,310 - +0.1 355,333 - -0.9 355,359 + +0.8 355,359 + +0.1 355,360 - -0.2 355,361 + -2.1 355,361 + +0.2 355,362 - -1.0 355,369 - +0.2 355,387 - +1.6 355,475 + +0.2 355,475 + +0.8 355,475 + +0.5 355,475 + +0.5 355,475 + +0.1 355,475 + +0.6 355,475 + +0.7 355,475 + +0.1 355,475 + +1.1 355,476 - +0.5 355,476 - -0.3 355,476 - -0.7 355,476 - +0.1 355,476 - +0.3 355,546 - -0.4 355,587 + +0.3 355,587 + -0.3 355,587 + +0.6 355,588 - -0.9 355,588 - -0.3 355,590 + -0.4 355,590 + +0.0 355,591 - +0.8 355,857 + RR42_RS01575 0.29 -1.5 355,857 + RR42_RS01575 0.29 +0.1 355,857 + RR42_RS01575 0.29 +0.3 355,858 - RR42_RS01575 0.29 -0.1 356,024 + RR42_RS01575 0.41 -0.4 356,024 + RR42_RS01575 0.41 +0.6 356,025 - RR42_RS01575 0.41 +2.3 356,040 + RR42_RS01575 0.42 -0.4 356,185 - RR42_RS01575 0.53 -0.3 356,437 - RR42_RS01575 0.72 +0.2 356,437 - RR42_RS01575 0.72 +0.1 356,437 - RR42_RS01575 0.72 +0.5 356,442 + RR42_RS01575 0.73 -0.5 356,443 - RR42_RS01575 0.73 -0.3 356,443 - RR42_RS01575 0.73 -0.7 356,443 - RR42_RS01575 0.73 -1.8 356,488 - RR42_RS01575 0.76 +0.4 356,489 + -0.7 356,489 + RR42_RS01575 0.76 -1.1 356,490 - RR42_RS01575 0.76 +0.1 356,490 - RR42_RS01575 0.76 -0.8 356,490 - RR42_RS01575 0.76 -0.0 356,493 + RR42_RS01575 0.76 +0.4 356,493 + RR42_RS01575 0.76 +0.7 356,493 + RR42_RS01575 0.76 +0.7 356,494 - RR42_RS01575 0.76 -2.2 356,494 - RR42_RS01575 0.76 -0.1 356,494 - RR42_RS01575 0.76 -0.3 356,494 - RR42_RS01575 0.76 +0.4 356,658 + RR42_RS01575 0.89 -0.1 356,897 - -0.6 357,381 + RR42_RS01580 0.35 +0.1 357,381 + RR42_RS01580 0.35 -0.4 357,381 + RR42_RS01580 0.35 -1.3 357,382 - RR42_RS01580 0.35 -0.3 357,571 + RR42_RS01580 0.47 +0.3 357,572 - RR42_RS01580 0.47 +0.6 357,578 - RR42_RS01580 0.48 +1.3 357,697 + RR42_RS01580 0.56 -0.7 357,698 - RR42_RS01580 0.56 +0.0
Or see this region's nucleotide sequence