Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01185 and RR42_RS01190 are separated by 246 nucleotides RR42_RS01190 and RR42_RS01195 are separated by 75 nucleotides RR42_RS01195 and RR42_RS01200 are separated by 64 nucleotides
RR42_RS01185: RR42_RS01185 - muropeptide transporter, at 271,272 to 272,528
_RS01185
RR42_RS01190: RR42_RS01190 - peptidase M48, at 272,775 to 273,722
_RS01190
RR42_RS01195: RR42_RS01195 - excinuclease ATPase, at 273,798 to 274,271
_RS01195
RR42_RS01200: RR42_RS01200 - hypothetical protein, at 274,336 to 275,145
_RS01200
Position (kb) 272
273
274 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 271.826 kb on - strand, within RR42_RS01185 at 271.996 kb on + strand, within RR42_RS01185 at 271.997 kb on - strand, within RR42_RS01185 at 271.997 kb on - strand, within RR42_RS01185 at 272.134 kb on + strand, within RR42_RS01185 at 272.135 kb on - strand, within RR42_RS01185 at 272.166 kb on + strand, within RR42_RS01185 at 272.166 kb on + strand, within RR42_RS01185 at 272.166 kb on + strand, within RR42_RS01185 at 272.167 kb on - strand, within RR42_RS01185 at 272.309 kb on + strand, within RR42_RS01185 at 272.310 kb on - strand, within RR42_RS01185 at 272.312 kb on - strand, within RR42_RS01185 at 272.312 kb on - strand, within RR42_RS01185 at 272.348 kb on + strand, within RR42_RS01185 at 272.348 kb on + strand, within RR42_RS01185 at 272.348 kb on + strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.351 kb on - strand, within RR42_RS01185 at 272.351 kb on - strand, within RR42_RS01185 at 272.351 kb on - strand, within RR42_RS01185 at 272.513 kb on - strand at 272.604 kb on - strand at 272.604 kb on - strand at 272.607 kb on - strand at 272.607 kb on - strand at 272.607 kb on - strand at 272.615 kb on - strand at 272.615 kb on - strand at 272.687 kb on - strand at 272.958 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.169 kb on - strand, within RR42_RS01190 at 273.344 kb on + strand, within RR42_RS01190 at 273.345 kb on - strand, within RR42_RS01190 at 273.345 kb on - strand, within RR42_RS01190 at 273.345 kb on - strand at 273.345 kb on - strand, within RR42_RS01190 at 273.447 kb on + strand, within RR42_RS01190 at 273.653 kb on + strand at 273.713 kb on - strand at 273.720 kb on + strand at 273.759 kb on - strand at 273.759 kb on - strand at 273.763 kb on + strand at 273.763 kb on + strand at 273.764 kb on - strand at 273.764 kb on - strand at 273.767 kb on + strand at 273.767 kb on + strand at 274.200 kb on + strand, within RR42_RS01195 at 274.200 kb on + strand, within RR42_RS01195 at 274.201 kb on - strand, within RR42_RS01195 at 274.224 kb on + strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 271,826 - RR42_RS01185 0.44 -1.0 271,996 + RR42_RS01185 0.58 -1.1 271,997 - RR42_RS01185 0.58 -0.4 271,997 - RR42_RS01185 0.58 -1.8 272,134 + RR42_RS01185 0.69 -2.4 272,135 - RR42_RS01185 0.69 +0.1 272,166 + RR42_RS01185 0.71 -2.0 272,166 + RR42_RS01185 0.71 -1.5 272,166 + RR42_RS01185 0.71 -2.2 272,167 - RR42_RS01185 0.71 +2.1 272,309 + RR42_RS01185 0.82 -0.4 272,310 - RR42_RS01185 0.83 -1.3 272,312 - RR42_RS01185 0.83 -0.5 272,312 - RR42_RS01185 0.83 -0.2 272,348 + RR42_RS01185 0.86 -0.7 272,348 + RR42_RS01185 0.86 +0.3 272,348 + RR42_RS01185 0.86 -0.2 272,349 - RR42_RS01185 0.86 -1.3 272,349 - RR42_RS01185 0.86 +0.5 272,349 - RR42_RS01185 0.86 -0.9 272,349 - RR42_RS01185 0.86 -0.0 272,349 - RR42_RS01185 0.86 -3.2 272,350 + RR42_RS01185 0.86 -1.6 272,350 + RR42_RS01185 0.86 -1.0 272,350 + RR42_RS01185 0.86 -1.2 272,350 + RR42_RS01185 0.86 -3.0 272,351 - RR42_RS01185 0.86 -1.5 272,351 - RR42_RS01185 0.86 -1.0 272,351 - RR42_RS01185 0.86 -0.3 272,513 - -0.1 272,604 - -0.1 272,604 - -0.2 272,607 - +1.5 272,607 - -0.0 272,607 - +0.5 272,615 - +0.9 272,615 - +0.2 272,687 - +0.2 272,958 + RR42_RS01190 0.19 +0.6 273,168 + RR42_RS01190 0.41 +0.5 273,168 + RR42_RS01190 0.41 -0.0 273,168 + RR42_RS01190 0.41 +0.4 273,168 + RR42_RS01190 0.41 +1.2 273,169 - RR42_RS01190 0.42 +2.1 273,344 + RR42_RS01190 0.60 +0.7 273,345 - RR42_RS01190 0.60 -0.3 273,345 - RR42_RS01190 0.60 -0.2 273,345 - +1.4 273,345 - RR42_RS01190 0.60 -0.2 273,447 + RR42_RS01190 0.71 +1.0 273,653 + +0.1 273,713 - -0.6 273,720 + +0.4 273,759 - +0.2 273,759 - -0.6 273,763 + -0.1 273,763 + +0.3 273,764 - -0.6 273,764 - +0.4 273,767 + -0.6 273,767 + +0.9 274,200 + RR42_RS01195 0.85 +0.6 274,200 + RR42_RS01195 0.85 +0.6 274,201 - RR42_RS01195 0.85 -0.3 274,224 + -0.2 274,305 - +0.4 274,305 - +0.3 274,305 - +1.0 274,305 - -0.2 274,305 - +0.5
Or see this region's nucleotide sequence