Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01180 and RR42_RS01185 are separated by 157 nucleotides RR42_RS01185 and RR42_RS01190 are separated by 246 nucleotides
RR42_RS01180: RR42_RS01180 - acyl-CoA thioesterase, at 270,284 to 271,114
_RS01180
RR42_RS01185: RR42_RS01185 - muropeptide transporter, at 271,272 to 272,528
_RS01185
RR42_RS01190: RR42_RS01190 - peptidase M48, at 272,775 to 273,722
_RS01190
Position (kb)
271
272
273 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 270.387 kb on - strand, within RR42_RS01180 at 270.737 kb on + strand, within RR42_RS01180 at 270.737 kb on + strand, within RR42_RS01180 at 270.737 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.945 kb on - strand, within RR42_RS01180 at 271.103 kb on + strand at 271.103 kb on + strand at 271.103 kb on + strand at 271.103 kb on + strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.112 kb on + strand at 271.112 kb on + strand at 271.112 kb on + strand at 271.113 kb on - strand at 271.113 kb on - strand at 271.113 kb on - strand at 271.171 kb on - strand at 271.171 kb on - strand at 271.233 kb on - strand at 271.273 kb on + strand at 271.300 kb on + strand at 271.330 kb on - strand at 271.339 kb on - strand at 271.372 kb on + strand at 271.373 kb on - strand at 271.426 kb on + strand, within RR42_RS01185 at 271.427 kb on - strand, within RR42_RS01185 at 271.427 kb on - strand, within RR42_RS01185 at 271.610 kb on - strand, within RR42_RS01185 at 271.660 kb on + strand, within RR42_RS01185 at 271.660 kb on + strand, within RR42_RS01185 at 271.826 kb on - strand, within RR42_RS01185 at 271.996 kb on + strand, within RR42_RS01185 at 271.997 kb on - strand, within RR42_RS01185 at 271.997 kb on - strand, within RR42_RS01185 at 272.134 kb on + strand, within RR42_RS01185 at 272.135 kb on - strand, within RR42_RS01185 at 272.166 kb on + strand, within RR42_RS01185 at 272.166 kb on + strand, within RR42_RS01185 at 272.166 kb on + strand, within RR42_RS01185 at 272.167 kb on - strand, within RR42_RS01185 at 272.309 kb on + strand, within RR42_RS01185 at 272.310 kb on - strand, within RR42_RS01185 at 272.312 kb on - strand, within RR42_RS01185 at 272.312 kb on - strand, within RR42_RS01185 at 272.348 kb on + strand, within RR42_RS01185 at 272.348 kb on + strand, within RR42_RS01185 at 272.348 kb on + strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.349 kb on - strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.350 kb on + strand, within RR42_RS01185 at 272.351 kb on - strand, within RR42_RS01185 at 272.351 kb on - strand, within RR42_RS01185 at 272.351 kb on - strand, within RR42_RS01185 at 272.513 kb on - strand at 272.604 kb on - strand at 272.604 kb on - strand at 272.607 kb on - strand at 272.607 kb on - strand at 272.607 kb on - strand at 272.615 kb on - strand at 272.615 kb on - strand at 272.687 kb on - strand at 272.958 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.169 kb on - strand, within RR42_RS01190 at 273.344 kb on + strand, within RR42_RS01190 at 273.345 kb on - strand, within RR42_RS01190 at 273.345 kb on - strand, within RR42_RS01190 at 273.345 kb on - strand at 273.345 kb on - strand, within RR42_RS01190 at 273.447 kb on + strand, within RR42_RS01190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 270,387 - RR42_RS01180 0.12 +0.3 270,737 + RR42_RS01180 0.55 -0.4 270,737 + RR42_RS01180 0.55 +1.2 270,737 + RR42_RS01180 0.55 +0.8 270,944 + RR42_RS01180 0.79 +1.7 270,944 + RR42_RS01180 0.79 +0.5 270,944 + RR42_RS01180 0.79 +1.6 270,944 + RR42_RS01180 0.79 +0.4 270,945 - RR42_RS01180 0.80 +1.7 271,103 + +0.5 271,103 + +0.5 271,103 + -0.0 271,103 + +0.2 271,104 - -0.1 271,104 - +0.1 271,104 - +0.6 271,104 - +0.0 271,104 - -0.8 271,112 + -0.8 271,112 + -0.4 271,112 + -0.1 271,113 - -0.0 271,113 - -0.3 271,113 - +0.0 271,171 - -0.0 271,171 - +0.3 271,233 - +0.5 271,273 + -0.1 271,300 + -1.1 271,330 - -0.6 271,339 - -0.4 271,372 + -1.8 271,373 - -2.8 271,426 + RR42_RS01185 0.12 -2.1 271,427 - RR42_RS01185 0.12 -0.4 271,427 - RR42_RS01185 0.12 -0.2 271,610 - RR42_RS01185 0.27 -1.6 271,660 + RR42_RS01185 0.31 -1.1 271,660 + RR42_RS01185 0.31 +0.7 271,826 - RR42_RS01185 0.44 -1.0 271,996 + RR42_RS01185 0.58 -1.1 271,997 - RR42_RS01185 0.58 -0.4 271,997 - RR42_RS01185 0.58 -1.8 272,134 + RR42_RS01185 0.69 -2.4 272,135 - RR42_RS01185 0.69 +0.1 272,166 + RR42_RS01185 0.71 -2.0 272,166 + RR42_RS01185 0.71 -1.5 272,166 + RR42_RS01185 0.71 -2.2 272,167 - RR42_RS01185 0.71 +2.1 272,309 + RR42_RS01185 0.82 -0.4 272,310 - RR42_RS01185 0.83 -1.3 272,312 - RR42_RS01185 0.83 -0.5 272,312 - RR42_RS01185 0.83 -0.2 272,348 + RR42_RS01185 0.86 -0.7 272,348 + RR42_RS01185 0.86 +0.3 272,348 + RR42_RS01185 0.86 -0.2 272,349 - RR42_RS01185 0.86 -1.3 272,349 - RR42_RS01185 0.86 +0.5 272,349 - RR42_RS01185 0.86 -0.9 272,349 - RR42_RS01185 0.86 -0.0 272,349 - RR42_RS01185 0.86 -3.2 272,350 + RR42_RS01185 0.86 -1.6 272,350 + RR42_RS01185 0.86 -1.0 272,350 + RR42_RS01185 0.86 -1.2 272,350 + RR42_RS01185 0.86 -3.0 272,351 - RR42_RS01185 0.86 -1.5 272,351 - RR42_RS01185 0.86 -1.0 272,351 - RR42_RS01185 0.86 -0.3 272,513 - -0.1 272,604 - -0.1 272,604 - -0.2 272,607 - +1.5 272,607 - -0.0 272,607 - +0.5 272,615 - +0.9 272,615 - +0.2 272,687 - +0.2 272,958 + RR42_RS01190 0.19 +0.6 273,168 + RR42_RS01190 0.41 +0.5 273,168 + RR42_RS01190 0.41 -0.0 273,168 + RR42_RS01190 0.41 +0.4 273,168 + RR42_RS01190 0.41 +1.2 273,169 - RR42_RS01190 0.42 +2.1 273,344 + RR42_RS01190 0.60 +0.7 273,345 - RR42_RS01190 0.60 -0.3 273,345 - RR42_RS01190 0.60 -0.2 273,345 - +1.4 273,345 - RR42_RS01190 0.60 -0.2 273,447 + RR42_RS01190 0.71 +1.0
Or see this region's nucleotide sequence