Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00985 and RR42_RS00990 are separated by 10 nucleotides RR42_RS00990 and RR42_RS00995 are separated by 158 nucleotides RR42_RS00995 and RR42_RS01000 are separated by 17 nucleotides
RR42_RS00985: RR42_RS00985 - acetyltransferase, at 231,882 to 232,496
_RS00985
RR42_RS00990: RR42_RS00990 - GntR family transcriptional regulator, at 232,507 to 233,244
_RS00990
RR42_RS00995: RR42_RS00995 - sodium:cation symporter, at 233,403 to 234,932
_RS00995
RR42_RS01000: RR42_RS01000 - hypothetical protein, at 234,950 to 237,358
_RS01000
Position (kb)
233
234
235 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 232.416 kb on + strand, within RR42_RS00985 at 232.442 kb on + strand at 232.442 kb on + strand at 232.443 kb on - strand at 232.443 kb on - strand at 232.443 kb on - strand at 232.508 kb on + strand at 232.508 kb on + strand at 232.537 kb on - strand at 232.553 kb on + strand at 232.554 kb on - strand at 232.751 kb on + strand, within RR42_RS00990 at 232.751 kb on + strand, within RR42_RS00990 at 232.751 kb on + strand, within RR42_RS00990 at 232.751 kb on + strand, within RR42_RS00990 at 232.890 kb on + strand, within RR42_RS00990 at 232.977 kb on - strand, within RR42_RS00990 at 232.992 kb on + strand, within RR42_RS00990 at 232.993 kb on - strand, within RR42_RS00990 at 232.993 kb on - strand, within RR42_RS00990 at 232.993 kb on - strand, within RR42_RS00990 at 233.247 kb on - strand at 233.452 kb on + strand at 233.457 kb on + strand at 233.457 kb on + strand at 233.458 kb on - strand at 233.458 kb on - strand at 233.458 kb on - strand at 233.458 kb on - strand at 233.608 kb on + strand, within RR42_RS00995 at 233.850 kb on - strand, within RR42_RS00995 at 233.933 kb on + strand, within RR42_RS00995 at 233.934 kb on - strand, within RR42_RS00995 at 234.030 kb on + strand, within RR42_RS00995 at 234.030 kb on + strand, within RR42_RS00995 at 234.031 kb on - strand, within RR42_RS00995 at 234.080 kb on + strand, within RR42_RS00995 at 234.080 kb on + strand, within RR42_RS00995 at 234.081 kb on - strand, within RR42_RS00995 at 234.130 kb on - strand, within RR42_RS00995 at 234.130 kb on - strand, within RR42_RS00995 at 234.159 kb on + strand, within RR42_RS00995 at 234.159 kb on + strand, within RR42_RS00995 at 234.160 kb on - strand, within RR42_RS00995 at 234.201 kb on + strand, within RR42_RS00995 at 234.201 kb on + strand, within RR42_RS00995 at 234.202 kb on - strand, within RR42_RS00995 at 234.202 kb on - strand, within RR42_RS00995 at 234.540 kb on + strand, within RR42_RS00995 at 234.738 kb on + strand, within RR42_RS00995 at 234.739 kb on - strand, within RR42_RS00995 at 234.739 kb on - strand, within RR42_RS00995 at 234.777 kb on + strand, within RR42_RS00995 at 234.779 kb on + strand, within RR42_RS00995 at 234.779 kb on + strand, within RR42_RS00995 at 234.876 kb on + strand at 234.877 kb on - strand at 234.877 kb on - strand at 234.877 kb on - strand at 234.877 kb on - strand at 234.877 kb on - strand at 234.882 kb on + strand at 234.882 kb on + strand at 234.882 kb on + strand at 234.883 kb on - strand at 234.883 kb on - strand at 234.883 kb on - strand at 235.031 kb on + strand at 235.031 kb on + strand at 235.031 kb on + strand at 235.032 kb on - strand at 235.124 kb on + strand at 235.124 kb on + strand at 235.124 kb on + strand at 235.124 kb on + strand at 235.185 kb on - strand at 235.232 kb on + strand, within RR42_RS01000 at 235.232 kb on + strand, within RR42_RS01000 at 235.233 kb on - strand, within RR42_RS01000 at 235.233 kb on - strand, within RR42_RS01000 at 235.233 kb on - strand, within RR42_RS01000 at 235.233 kb on - strand, within RR42_RS01000 at 235.454 kb on - strand, within RR42_RS01000 at 235.481 kb on + strand, within RR42_RS01000 at 235.481 kb on + strand, within RR42_RS01000 at 235.481 kb on + strand, within RR42_RS01000 at 235.481 kb on + strand, within RR42_RS01000 at 235.482 kb on - strand, within RR42_RS01000 at 235.482 kb on - strand, within RR42_RS01000 at 235.495 kb on - strand, within RR42_RS01000 at 235.645 kb on + strand, within RR42_RS01000 at 235.645 kb on + strand, within RR42_RS01000 at 235.645 kb on + strand, within RR42_RS01000 at 235.646 kb on - strand, within RR42_RS01000 at 235.672 kb on - strand, within RR42_RS01000 at 235.880 kb on + strand, within RR42_RS01000 at 235.880 kb on + strand, within RR42_RS01000 at 235.880 kb on + strand, within RR42_RS01000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 232,416 + RR42_RS00985 0.87 +0.5 232,442 + -0.2 232,442 + +1.2 232,443 - +0.7 232,443 - +0.0 232,443 - -0.8 232,508 + -0.0 232,508 + -0.7 232,537 - -0.2 232,553 + +1.0 232,554 - -0.6 232,751 + RR42_RS00990 0.33 +0.2 232,751 + RR42_RS00990 0.33 -0.1 232,751 + RR42_RS00990 0.33 -0.0 232,751 + RR42_RS00990 0.33 +0.5 232,890 + RR42_RS00990 0.52 +1.4 232,977 - RR42_RS00990 0.64 +0.7 232,992 + RR42_RS00990 0.66 -0.0 232,993 - RR42_RS00990 0.66 +1.0 232,993 - RR42_RS00990 0.66 -0.2 232,993 - RR42_RS00990 0.66 +0.5 233,247 - -0.9 233,452 + +0.4 233,457 + +0.8 233,457 + +0.7 233,458 - -0.2 233,458 - -0.9 233,458 - -0.3 233,458 - -0.1 233,608 + RR42_RS00995 0.13 -1.9 233,850 - RR42_RS00995 0.29 -1.0 233,933 + RR42_RS00995 0.35 -0.5 233,934 - RR42_RS00995 0.35 +0.0 234,030 + RR42_RS00995 0.41 +0.3 234,030 + RR42_RS00995 0.41 +0.5 234,031 - RR42_RS00995 0.41 +0.9 234,080 + RR42_RS00995 0.44 -0.2 234,080 + RR42_RS00995 0.44 -0.3 234,081 - RR42_RS00995 0.44 -0.2 234,130 - RR42_RS00995 0.48 -1.2 234,130 - RR42_RS00995 0.48 -0.9 234,159 + RR42_RS00995 0.49 -0.7 234,159 + RR42_RS00995 0.49 -0.0 234,160 - RR42_RS00995 0.49 -0.9 234,201 + RR42_RS00995 0.52 -0.2 234,201 + RR42_RS00995 0.52 +1.0 234,202 - RR42_RS00995 0.52 +0.6 234,202 - RR42_RS00995 0.52 -0.2 234,540 + RR42_RS00995 0.74 -1.0 234,738 + RR42_RS00995 0.87 +0.2 234,739 - RR42_RS00995 0.87 +0.1 234,739 - RR42_RS00995 0.87 -0.2 234,777 + RR42_RS00995 0.90 -0.0 234,779 + RR42_RS00995 0.90 -1.0 234,779 + RR42_RS00995 0.90 +1.0 234,876 + -0.9 234,877 - +0.7 234,877 - +0.9 234,877 - +0.4 234,877 - +0.7 234,877 - +0.2 234,882 + -0.3 234,882 + -0.3 234,882 + -0.3 234,883 - +0.4 234,883 - +0.6 234,883 - +0.6 235,031 + +0.5 235,031 + +0.2 235,031 + +1.3 235,032 - +0.6 235,124 + -0.5 235,124 + -0.6 235,124 + -0.6 235,124 + -0.5 235,185 - +2.1 235,232 + RR42_RS01000 0.12 -0.8 235,232 + RR42_RS01000 0.12 -1.2 235,233 - RR42_RS01000 0.12 -0.1 235,233 - RR42_RS01000 0.12 -0.6 235,233 - RR42_RS01000 0.12 +0.4 235,233 - RR42_RS01000 0.12 +0.2 235,454 - RR42_RS01000 0.21 +0.4 235,481 + RR42_RS01000 0.22 -0.3 235,481 + RR42_RS01000 0.22 +0.5 235,481 + RR42_RS01000 0.22 +0.4 235,481 + RR42_RS01000 0.22 +0.1 235,482 - RR42_RS01000 0.22 +2.4 235,482 - RR42_RS01000 0.22 +1.7 235,495 - RR42_RS01000 0.23 -0.8 235,645 + RR42_RS01000 0.29 -0.7 235,645 + RR42_RS01000 0.29 -0.7 235,645 + RR42_RS01000 0.29 -0.2 235,646 - RR42_RS01000 0.29 +1.7 235,672 - RR42_RS01000 0.30 +0.8 235,880 + RR42_RS01000 0.39 -0.2 235,880 + RR42_RS01000 0.39 +0.2 235,880 + RR42_RS01000 0.39 -0.3
Or see this region's nucleotide sequence