Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00600 and RR42_RS00605 are separated by 106 nucleotides RR42_RS00605 and RR42_RS00610 are separated by 169 nucleotides RR42_RS00610 and RR42_RS00615 are separated by 35 nucleotides
RR42_RS00600: RR42_RS00600 - DeoR family transcriptional regulator, at 157,874 to 158,845
_RS00600
RR42_RS00605: RR42_RS00605 - glutamyl-tRNA amidotransferase, at 158,952 to 160,412
_RS00605
RR42_RS00610: RR42_RS00610 - hypothetical protein, at 160,582 to 161,250
_RS00610
RR42_RS00615: RR42_RS00615 - recombinase XerC, at 161,286 to 162,410
_RS00615
Position (kb)
158
159
160
161 Strain fitness (log2 ratio)
-1
0
1 at 158.678 kb on + strand, within RR42_RS00600 at 158.855 kb on + strand at 158.861 kb on + strand at 158.861 kb on + strand at 158.861 kb on + strand at 158.865 kb on + strand at 158.865 kb on + strand at 158.865 kb on + strand at 158.905 kb on + strand at 158.905 kb on + strand at 158.905 kb on + strand at 158.916 kb on + strand at 158.916 kb on + strand at 158.916 kb on + strand at 158.926 kb on + strand at 158.926 kb on + strand at 158.926 kb on + strand at 160.410 kb on + strand at 160.420 kb on - strand at 160.457 kb on + strand at 160.458 kb on - strand at 160.550 kb on - strand at 160.606 kb on + strand at 160.606 kb on + strand at 160.607 kb on - strand at 160.861 kb on + strand at 160.862 kb on - strand, within RR42_RS00610 at 160.939 kb on + strand, within RR42_RS00610 at 160.939 kb on + strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 161.011 kb on + strand, within RR42_RS00610 at 161.195 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 158,678 + RR42_RS00600 0.83 -0.5 158,855 + +0.1 158,861 + +0.7 158,861 + -0.8 158,861 + -1.4 158,865 + -1.0 158,865 + -0.5 158,865 + +0.6 158,905 + -0.6 158,905 + +0.3 158,905 + +0.0 158,916 + +0.5 158,916 + -0.4 158,916 + -0.1 158,926 + -0.0 158,926 + +0.1 158,926 + +0.7 160,410 + -0.5 160,420 - -0.5 160,457 + -0.2 160,458 - -0.6 160,550 - +0.3 160,606 + +0.8 160,606 + -1.0 160,607 - -0.9 160,861 + +1.4 160,862 - RR42_RS00610 0.42 +0.1 160,939 + RR42_RS00610 0.53 -1.6 160,939 + RR42_RS00610 0.53 -1.5 160,940 - RR42_RS00610 0.54 -0.6 160,940 - RR42_RS00610 0.54 +0.0 160,940 - RR42_RS00610 0.54 +0.9 160,940 - RR42_RS00610 0.54 -0.4 161,011 + RR42_RS00610 0.64 +0.1 161,195 - -0.3
Or see this region's nucleotide sequence