Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00540 and RR42_RS00545 are separated by 83 nucleotides RR42_RS00545 and RR42_RS00550 are separated by 78 nucleotides
RR42_RS00540: RR42_RS00540 - hypothetical protein, at 142,940 to 145,594
_RS00540
RR42_RS00545: RR42_RS00545 - transglutaminase, at 145,678 to 146,553
_RS00545
RR42_RS00550: RR42_RS00550 - hypothetical protein, at 146,632 to 147,564
_RS00550
Position (kb) 145
146
147 Strain fitness (log2 ratio) -2
-1
0
1 at 144.852 kb on + strand, within RR42_RS00540 at 144.853 kb on - strand, within RR42_RS00540 at 144.989 kb on + strand, within RR42_RS00540 at 144.990 kb on - strand, within RR42_RS00540 at 144.990 kb on - strand, within RR42_RS00540 at 144.990 kb on - strand, within RR42_RS00540 at 144.990 kb on - strand, within RR42_RS00540 at 144.990 kb on - strand, within RR42_RS00540 at 145.469 kb on + strand at 145.469 kb on + strand at 145.469 kb on + strand at 145.488 kb on + strand at 145.705 kb on + strand at 145.705 kb on + strand at 145.705 kb on + strand at 145.706 kb on - strand at 145.706 kb on - strand at 145.706 kb on - strand at 145.711 kb on + strand at 145.712 kb on - strand at 145.862 kb on - strand, within RR42_RS00545 at 145.862 kb on - strand, within RR42_RS00545 at 145.888 kb on + strand, within RR42_RS00545 at 145.888 kb on + strand, within RR42_RS00545 at 145.889 kb on - strand, within RR42_RS00545 at 146.162 kb on - strand, within RR42_RS00545 at 146.288 kb on - strand, within RR42_RS00545 at 146.465 kb on - strand, within RR42_RS00545 at 146.466 kb on + strand at 146.467 kb on - strand at 146.551 kb on + strand at 146.552 kb on - strand at 146.598 kb on + strand at 146.599 kb on - strand at 146.636 kb on + strand at 146.636 kb on + strand at 146.636 kb on + strand at 146.636 kb on + strand at 146.637 kb on - strand at 146.637 kb on - strand at 146.637 kb on - strand at 146.719 kb on + strand at 146.719 kb on + strand at 146.719 kb on + strand at 146.720 kb on - strand at 146.720 kb on - strand at 147.062 kb on + strand, within RR42_RS00550 at 147.063 kb on - strand, within RR42_RS00550 at 147.063 kb on - strand, within RR42_RS00550 at 147.107 kb on - strand, within RR42_RS00550 at 147.107 kb on - strand, within RR42_RS00550 at 147.107 kb on - strand, within RR42_RS00550 at 147.214 kb on + strand, within RR42_RS00550 at 147.214 kb on + strand, within RR42_RS00550 at 147.214 kb on + strand, within RR42_RS00550 at 147.215 kb on - strand, within RR42_RS00550 at 147.215 kb on - strand, within RR42_RS00550 at 147.215 kb on - strand, within RR42_RS00550 at 147.259 kb on + strand, within RR42_RS00550 at 147.259 kb on + strand, within RR42_RS00550 at 147.260 kb on - strand, within RR42_RS00550 at 147.260 kb on - strand, within RR42_RS00550 at 147.260 kb on - strand, within RR42_RS00550 at 147.260 kb on - strand, within RR42_RS00550 at 147.272 kb on - strand, within RR42_RS00550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 144,852 + RR42_RS00540 0.72 -1.9 144,853 - RR42_RS00540 0.72 -0.2 144,989 + RR42_RS00540 0.77 -0.2 144,990 - RR42_RS00540 0.77 -0.5 144,990 - RR42_RS00540 0.77 -0.6 144,990 - RR42_RS00540 0.77 -0.5 144,990 - RR42_RS00540 0.77 -1.3 144,990 - RR42_RS00540 0.77 -0.9 145,469 + -0.1 145,469 + +0.4 145,469 + -0.4 145,488 + -0.5 145,705 + +0.5 145,705 + +0.4 145,705 + -0.8 145,706 - +0.5 145,706 - -1.2 145,706 - -1.9 145,711 + -1.1 145,712 - +0.2 145,862 - RR42_RS00545 0.21 -0.1 145,862 - RR42_RS00545 0.21 -0.5 145,888 + RR42_RS00545 0.24 -1.0 145,888 + RR42_RS00545 0.24 -1.3 145,889 - RR42_RS00545 0.24 -0.8 146,162 - RR42_RS00545 0.55 -0.3 146,288 - RR42_RS00545 0.70 -0.5 146,465 - RR42_RS00545 0.90 -0.7 146,466 + -0.7 146,467 - -0.0 146,551 + -0.8 146,552 - -0.8 146,598 + -0.3 146,599 - -0.7 146,636 + +0.8 146,636 + +1.2 146,636 + -0.4 146,636 + -0.5 146,637 - -0.4 146,637 - -0.1 146,637 - -1.1 146,719 + +0.0 146,719 + -1.3 146,719 + +0.2 146,720 - -1.5 146,720 - -0.4 147,062 + RR42_RS00550 0.46 -1.1 147,063 - RR42_RS00550 0.46 -0.5 147,063 - RR42_RS00550 0.46 -2.2 147,107 - RR42_RS00550 0.51 -0.6 147,107 - RR42_RS00550 0.51 +0.2 147,107 - RR42_RS00550 0.51 +0.1 147,214 + RR42_RS00550 0.62 -1.4 147,214 + RR42_RS00550 0.62 -0.0 147,214 + RR42_RS00550 0.62 +1.5 147,215 - RR42_RS00550 0.62 -1.5 147,215 - RR42_RS00550 0.62 -0.0 147,215 - RR42_RS00550 0.62 +0.7 147,259 + RR42_RS00550 0.67 -0.2 147,259 + RR42_RS00550 0.67 +0.0 147,260 - RR42_RS00550 0.67 -2.0 147,260 - RR42_RS00550 0.67 +0.7 147,260 - RR42_RS00550 0.67 -2.1 147,260 - RR42_RS00550 0.67 -0.1 147,272 - RR42_RS00550 0.69 -0.9
Or see this region's nucleotide sequence