Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00535 and RR42_RS00540 are separated by 26 nucleotides RR42_RS00540 and RR42_RS00545 are separated by 83 nucleotides
RR42_RS00535: RR42_RS00535 - IMP dehydrogenase, at 139,371 to 142,913
_RS00535
RR42_RS00540: RR42_RS00540 - hypothetical protein, at 142,940 to 145,594
_RS00540
RR42_RS00545: RR42_RS00545 - transglutaminase, at 145,678 to 146,553
_RS00545
Position (kb)
142
143
144
145
146 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 142.026 kb on - strand, within RR42_RS00535 at 142.219 kb on - strand, within RR42_RS00535 at 142.486 kb on - strand, within RR42_RS00535 at 142.624 kb on - strand at 142.734 kb on + strand at 142.735 kb on - strand at 142.735 kb on - strand at 142.760 kb on + strand at 142.760 kb on + strand at 142.761 kb on - strand at 142.764 kb on + strand at 142.764 kb on + strand at 142.764 kb on + strand at 142.764 kb on + strand at 142.764 kb on + strand at 142.764 kb on + strand at 142.764 kb on + strand at 142.765 kb on - strand at 142.765 kb on - strand at 142.788 kb on + strand at 143.091 kb on + strand at 143.092 kb on - strand at 143.171 kb on + strand at 143.171 kb on + strand at 143.172 kb on - strand at 143.172 kb on - strand at 143.172 kb on - strand at 143.324 kb on + strand, within RR42_RS00540 at 143.324 kb on + strand, within RR42_RS00540 at 143.324 kb on + strand, within RR42_RS00540 at 143.325 kb on - strand, within RR42_RS00540 at 143.325 kb on - strand, within RR42_RS00540 at 143.412 kb on - strand, within RR42_RS00540 at 143.412 kb on - strand, within RR42_RS00540 at 143.464 kb on + strand, within RR42_RS00540 at 143.464 kb on + strand, within RR42_RS00540 at 143.465 kb on - strand, within RR42_RS00540 at 143.465 kb on - strand, within RR42_RS00540 at 143.465 kb on - strand, within RR42_RS00540 at 143.525 kb on + strand, within RR42_RS00540 at 143.729 kb on + strand, within RR42_RS00540 at 143.730 kb on - strand, within RR42_RS00540 at 143.732 kb on - strand, within RR42_RS00540 at 143.741 kb on + strand, within RR42_RS00540 at 143.742 kb on - strand, within RR42_RS00540 at 143.825 kb on + strand, within RR42_RS00540 at 143.825 kb on + strand, within RR42_RS00540 at 143.826 kb on - strand, within RR42_RS00540 at 143.826 kb on - strand, within RR42_RS00540 at 143.889 kb on - strand, within RR42_RS00540 at 143.889 kb on - strand, within RR42_RS00540 at 144.158 kb on + strand, within RR42_RS00540 at 144.323 kb on - strand, within RR42_RS00540 at 144.353 kb on + strand, within RR42_RS00540 at 144.353 kb on + strand, within RR42_RS00540 at 144.353 kb on + strand, within RR42_RS00540 at 144.354 kb on - strand, within RR42_RS00540 at 144.633 kb on + strand, within RR42_RS00540 at 144.633 kb on + strand, within RR42_RS00540 at 144.634 kb on - strand, within RR42_RS00540 at 144.634 kb on - strand, within RR42_RS00540 at 144.852 kb on + strand, within RR42_RS00540 at 144.853 kb on - strand, within RR42_RS00540 at 144.989 kb on + strand, within RR42_RS00540 at 144.990 kb on - strand, within RR42_RS00540 at 144.990 kb on - strand, within RR42_RS00540 at 144.990 kb on - strand, within RR42_RS00540 at 144.990 kb on - strand, within RR42_RS00540 at 144.990 kb on - strand, within RR42_RS00540 at 145.469 kb on + strand at 145.469 kb on + strand at 145.469 kb on + strand at 145.488 kb on + strand at 145.705 kb on + strand at 145.705 kb on + strand at 145.705 kb on + strand at 145.706 kb on - strand at 145.706 kb on - strand at 145.706 kb on - strand at 145.711 kb on + strand at 145.712 kb on - strand at 145.862 kb on - strand, within RR42_RS00545 at 145.862 kb on - strand, within RR42_RS00545 at 145.888 kb on + strand, within RR42_RS00545 at 145.888 kb on + strand, within RR42_RS00545 at 145.889 kb on - strand, within RR42_RS00545 at 146.162 kb on - strand, within RR42_RS00545 at 146.288 kb on - strand, within RR42_RS00545 at 146.465 kb on - strand, within RR42_RS00545 at 146.466 kb on + strand at 146.467 kb on - strand at 146.551 kb on + strand at 146.552 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 142,026 - RR42_RS00535 0.75 +0.1 142,219 - RR42_RS00535 0.80 -1.3 142,486 - RR42_RS00535 0.88 -0.3 142,624 - -1.6 142,734 + -0.6 142,735 - +0.4 142,735 - +0.1 142,760 + +2.4 142,760 + -0.6 142,761 - -1.2 142,764 + +0.5 142,764 + -0.1 142,764 + -0.3 142,764 + -1.9 142,764 + -0.1 142,764 + -0.2 142,764 + +0.4 142,765 - +0.7 142,765 - +1.2 142,788 + -2.2 143,091 + -1.6 143,092 - -0.1 143,171 + +0.1 143,171 + -0.7 143,172 - -1.0 143,172 - -0.1 143,172 - +0.4 143,324 + RR42_RS00540 0.14 -0.2 143,324 + RR42_RS00540 0.14 -1.1 143,324 + RR42_RS00540 0.14 +0.7 143,325 - RR42_RS00540 0.15 -0.1 143,325 - RR42_RS00540 0.15 -0.2 143,412 - RR42_RS00540 0.18 -0.2 143,412 - RR42_RS00540 0.18 -2.2 143,464 + RR42_RS00540 0.20 -0.4 143,464 + RR42_RS00540 0.20 -0.1 143,465 - RR42_RS00540 0.20 -0.2 143,465 - RR42_RS00540 0.20 +0.1 143,465 - RR42_RS00540 0.20 -0.7 143,525 + RR42_RS00540 0.22 -1.9 143,729 + RR42_RS00540 0.30 +4.2 143,730 - RR42_RS00540 0.30 +0.0 143,732 - RR42_RS00540 0.30 -1.2 143,741 + RR42_RS00540 0.30 +0.2 143,742 - RR42_RS00540 0.30 -0.9 143,825 + RR42_RS00540 0.33 +1.4 143,825 + RR42_RS00540 0.33 +1.0 143,826 - RR42_RS00540 0.33 -0.5 143,826 - RR42_RS00540 0.33 +0.8 143,889 - RR42_RS00540 0.36 -0.3 143,889 - RR42_RS00540 0.36 -0.7 144,158 + RR42_RS00540 0.46 -0.6 144,323 - RR42_RS00540 0.52 +0.4 144,353 + RR42_RS00540 0.53 +0.4 144,353 + RR42_RS00540 0.53 +0.0 144,353 + RR42_RS00540 0.53 -0.0 144,354 - RR42_RS00540 0.53 +0.8 144,633 + RR42_RS00540 0.64 +0.3 144,633 + RR42_RS00540 0.64 -0.4 144,634 - RR42_RS00540 0.64 +1.2 144,634 - RR42_RS00540 0.64 -0.6 144,852 + RR42_RS00540 0.72 -1.9 144,853 - RR42_RS00540 0.72 -0.2 144,989 + RR42_RS00540 0.77 -0.2 144,990 - RR42_RS00540 0.77 -0.5 144,990 - RR42_RS00540 0.77 -0.6 144,990 - RR42_RS00540 0.77 -0.5 144,990 - RR42_RS00540 0.77 -1.3 144,990 - RR42_RS00540 0.77 -0.9 145,469 + -0.1 145,469 + +0.4 145,469 + -0.4 145,488 + -0.5 145,705 + +0.5 145,705 + +0.4 145,705 + -0.8 145,706 - +0.5 145,706 - -1.2 145,706 - -1.9 145,711 + -1.1 145,712 - +0.2 145,862 - RR42_RS00545 0.21 -0.1 145,862 - RR42_RS00545 0.21 -0.5 145,888 + RR42_RS00545 0.24 -1.0 145,888 + RR42_RS00545 0.24 -1.3 145,889 - RR42_RS00545 0.24 -0.8 146,162 - RR42_RS00545 0.55 -0.3 146,288 - RR42_RS00545 0.70 -0.5 146,465 - RR42_RS00545 0.90 -0.7 146,466 + -0.7 146,467 - -0.0 146,551 + -0.8 146,552 - -0.8
Or see this region's nucleotide sequence